Fractionation and identification of ACE-inhibitory peptides from α-lactalbumin and β-casein produced by thermolysin-catalysed hydrolysis

Otte, Jeanette; Shalaby, Samah M.; Zakora, M.; Nielsen, Mette S.

doi:10.1016/j.idairyj.2007.04.008

Cited by 104 publications

(58 citation statements)

References 39 publications

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“…Among the sixteen hydrolysates evaluated (Table III), seven demonstrated high ACE-IA (> 80%: H1, H2, H3, H5, H6, H7 and H8), and four demonstrated greater activity (> 70%: H4, H13, H14 and H16) than the range of values reported in the literature (Otte et al, 2007). Three hydrolysates (H9, H11 and H15) displayed moderate inhibitory activity (40-60%) and only two samples (H10 and H12) demonstrated a low ability to inhibit ACE (<20%).…”

Section: Ace-inhibitory Activity Of Wpc Hydrolysatesmentioning

confidence: 76%

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Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity

Silvestre

Silva

et al. 2012

Braz. J. Pharm. Sci.

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The aim of this study was to prepare enzymatic hydrolysates from whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE) activity. The effects of the type of enzyme used (pancreatin or papain), the enzyme:substrate ratio (E:S ratio=0.5:100, 1:100, 2:100 and 3:100) and the use of ultrafiltration (UF) were investigated. The fractionation of peptides was performed by size-exclusion-HPLC, and the quantification of the components of the chromatographic fractions was carried out by a rapid Corrected Fraction Area method. The ACE inhibitory activity (ACE-IA) was determined by Reverse Phase-HPLC. All parameters tested affected both the peptide profile and the ACE-IA. The best peptide profile was achieved for the hydrolysates obtained with papain, whereas pancreatin was more advantageous in terms of ACE-IA. The beneficial effect of using a lower E:S ratio on the peptide profile and ACE-IA was observed for both enzymes depending on the conditions used to prepare the hydrolysates. The beneficial effect of not using UF on the peptide profile was observed in some cases for pancreatin and papain. However, the absence of UF yielded greater ACE-IA only when using papain.

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Section: Ace-inhibitory Activity Of Wpc Hydrolysatesmentioning

confidence: 76%

“…Hydrolysates and peptides derived from milk proteins may exhibit inhibitory activity (IA) against ACE. Thus, these proteins could be introduced into the diet as an alternative, non-pharmacological approach to preventing and treating arterial hypertension (Costa, Gontijo, Netto, 2007;Jiang et al, 2007;Miguel et al, 2007;Otte et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity

Silvestre

Silva

et al. 2012

Braz. J. Pharm. Sci.

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“…Dentre os doze hidrolisados avaliados, sete apresentaram AI que poderia ser considerada muito alta (> 80%: H1 a H6; H12), e quatro que poderia ser classificada como alta (> 70%: H8 a H11), quando comparados com as faixas de valores relatadas na literatura (OTTE et al, 2007). Apenas um hidrolisado (H7) revelou atividade inibitória moderada (na faixa de 60%) e nenhuma amostra demonstrou possuir baixa capacidade para inibir a ECA (< 20%).…”

Section: Resultsunclassified

“…Por outro lado, Otte et al (2007) relatam que os peptídeos mais potentes obtidos pela hidrólise enzimática da α-lactoalbumina e da β-caseína, apresentaram massas moleculares ao redor de 1.000 Da e 2.000 Da, respectivamente, demonstrando que mesmo peptídeos contendo acima de doze resíduos de aminoácidos poderiam apresentar atividade inibitória da ECA considerável.…”

Section: Resultsunclassified

“…Uma das mais importantes fontes de peptí-deos bioativos constitui nas proteínas do leite, sendo que em vários estudos já foi relatada a atividade inibitória de hidrolisados e peptídeos obtidos dessas proteínas sobre a ECA, sugerindo-se que estes produtos podem ser introduzidos na dieta, representando assim uma forma alternativa na prevenção e no tratamento não medicamentoso da hipertensão arterial (COSTA et al, 2007;JIANG et al, 2007;MIGUEL et al, 2007;OTTE et al, 2007 A enzima conversora de angiotensina -ECA (E.C 3.4.15.1, 0,25 U.mg -1 ), o hipuril-histidil-leucina (HHL) e o ácido hipúrico (AH) foram adquiridos da Sigma (Saint Louis, MO, EUA). O ácido trifluoracético e o ácido acético, ambos de grau HPLC, foram obtidos da Vetec (Duque de Caxias, RJ, Brasil).…”

Section: Introductionunclassified

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