Free Radical Scavenging Activity of Essential Oil of Eugenia caryophylata from Amboina Island and Derivatives of Eugenol

Abstract

Essential oil from Eugenia caryophylata was normally used to heal many different deseaces. Various chemical compositions of essential oil distilled and steamed of Moluccas Eugenia caryophylata has been investigated by many different researchers. Even though an intensive research has been carried out of the local chemotypes, a very detail study has not been fully investigated to find out the complete chemical compounds from the plant essential oil and its content associated with their biological activities. In present paper, we assess the free radical scavenging of E. caryophylata collected from Moluccas islands, Indonesia. Essential oil was extracted from leaves, buds, and stems of plant by steam distillation and analyzed using GC-FID and GC-MS. The result showed that free radical activity of essential oil, main constituent and its derivatives were analized using in vitro method. Essential oil activity from stem obtained as (0.82±0.15 μg/mL) was higher than that from bud and leaf possessing both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2’-azino-bis-3-ethylbenzthizoline-6-sulphonic acid (ABTS) radical scavenging assays by sinergism of eugenol, eugenyl acetate, β-caryophylene and humulene. The activity of isoeugenol (2) (3.59±0.54 μM) and (5.0±0.53 µM) scavenging DPPH and ABTS, respectively, as derivatives eugenol was higher than (3), (4) and (5). Although (6) was active originally, it was inactive after conversion of the ester. While the change of the double bond of location to conjungation structure caused more activity scavenging radicals than the starting molecule.

Evaluation of novel green walnut liqueur as a source of antioxidants: Multi-method approach

“…It was reported that the oxygenated-type of monoterpenes with phenolic structure, such as eugenol, found in GWL in major percentage, have the highest AO activity within the monoterpenes group (Gonzalez-Burgos and Gomez-Serranillos 2012). Scavenging activity of eugenol and its derivatives methyl and isomethyl eugenol towards DPPH and ABTS was higher than ascorbic acid (Sohilait and Kainama 2019). The hypothesis that eugenol reduces two or more DPPH radicals, despite the availability of only one hydrogen from a hydroxyl group was proposed as well as the formation of dimers (dehydrodieugenol) with two phenolic hydroxyl (Cortés-Rojas et al 2014).…”

Biological Activity of Extracts from Aromatic Plants as Control Agents against Spoilage Molds Isolated from Sheep Cheese

“…A similar behavior was observed in the EEs, with the lowest IC 50 values obtained in oregano (0.12 mg/mL), savory (2.32 mg/mL) and Spanish lavender (2.97 mg/mL) and the highest with tarragon (4.86 mg/mL) and hyssop (9.24 mg/mL). The low IC 50 values of oregano, savory and tarragon essential oils are mainly due to the presence of carvacrol and methyl eugenol in their composition ( Table 2 ) since other authors have related these compounds to low IC 50 values [ 81 , 82 ]. The lowest IC 50 value of the ethanolic extracts was obtained with oregano EE and this may be due to the fact that this contains the highest amount of LMWPC ( Table 3 ).…”

Chitin Nanocrystals Provide Antioxidant Activity to Polylactic Acid Films

“…The antioxidant activity of ChNC is rather low compared to conventional antioxidants such as ascorbic acid, essential oils, tocopherols, or phenolics that commonly show a DPPH inhibition up to 75–90% at low concentrations (0.1–1 mg/mL) [ 57 , 58 ]. However, if these antioxidants were to be used as part of packing concepts, they would need to be resistant to the processing conditions used during the production of thermoplastic food packages.…”