This study aims to investigate the antibacterial activities of essential oils isolated from various parts (buds, leaves and stems) of Eugenia caryophylata.The essentials oils were distillated by steam distillation, and the isolated was analyzed by GC and GC-MS. Five components of each sample of oils (buds, leaves and stems) were identified. Main component in the bud are eugenol (75.30%), eugenyl acetate (20.93%) and β-caryophyllene (3.00%) and eugenol (82.97%), β-caryophyllene (12.84%) in leaf oil, while in stem oil eugenol (97.75%). The oils were tested for in vitro antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), the Gram-negative bacteria (Escherichia coli and Salmonella typhimurium). The essential oils of bud, leaf and stem oil show that antibacterial activity against Gram-positive and Gram-negative bacteria.

Essential oil from Eugenia caryophylata was normally used to heal many different deseaces. Various chemical compositions of essential oil distilled and steamed of Moluccas Eugenia caryophylata has been investigated by many different researchers. Even though an intensive research has been carried out of the local chemotypes, a very detail study has not been fully investigated to find out the complete chemical compounds from the plant essential oil and its content associated with their biological activities. In present paper, we assess the free radical scavenging of E. caryophylata collected from Moluccas islands, Indonesia. Essential oil was extracted from leaves, buds, and stems of plant by steam distillation and analyzed using GC-FID and GC-MS. The result showed that free radical activity of essential oil, main constituent and its derivatives were analized using in vitro method. Essential oil activity from stem obtained as (0.82±0.15 μg/mL) was higher than that from bud and leaf possessing both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2’-azino-bis-3-ethylbenzthizoline-6-sulphonic acid (ABTS) radical scavenging assays by sinergism of eugenol, eugenyl acetate, β-caryophylene and humulene. The activity of isoeugenol (2) (3.59±0.54 μM) and (5.0±0.53 µM) scavenging DPPH and ABTS, respectively, as derivatives eugenol was higher than (3), (4) and (5). Although (6) was active originally, it was inactive after conversion of the ester. While the change of the double bond of location to conjungation structure caused more activity scavenging radicals than the starting molecule.

The essentials oils in Eugenia caryophylata, Thunb from Amboina Island on the bud, leaf and stem by steam distillation were isolated. Result of isolation was analyzed by GC and GC-MS. Five component were identified in the bud, leaf and stem oil. Main chemical component in the bud oil are eugenol (81.13-84.44 %), eugenyl acetate (11.60-15.02 %) and βcaryophyllene (3.45-4.60 %) respectively and eugenol (81.06-86.04 %), β-caryophyllene (11.95-16.16%), eugenyl acetate (2.02-3.05%) in leaf oil, while in stem oil eugenol (97.20-98.83%).

The Synthesis of 3.4-methylenedioxyphenyl-2-propanone from safrole has been achieved through conversion of allyl group to secondary alcohol, followed by oxidation with pyridinium chlorochromate(PCC).  The secondary alcohol has been achieved by two methods. The first method was formic acid adition reaction, followed by hydrolysis in aqueous ethanolic solution of potassium hydroxide.  The second method was the oxymercuration-demercuration reaction of safrole. The addition reaction of safrole with formic acid yield safrylformate (34,70%). The hydrolysis of safrylformate with 3M KOH produced safrylalchohol (73,29%). The oxymercuration-demercuration reaction of safrole with Hg(OAc)2-NaBH4 gave (74,37%) of safrylalcohol.  The oxidation of safryalcohol with PCC gave 3.4-methylenedioxyphenyl-2-propanone as a main target in 71,83%. The structure elucidations of these products were analyzed by  FTIR , 1H-NMR,  13C-NMR and MS.   Keyword: 3.4-methylenedioxyphenyl-2-propanone;  safrole

A healthy simple natural drink is introduced based on a deep physical research investigation into insulin leaf (Tithonia diversifolia (Hemsley) A. Gray (Asteraceae)). Such drink was simply fabricated using two steps process of heating and cooling, and then exposed according to 2 main unique physical properties of optical and electrical behaviors. It was well-known that Tithonia diversifolia (Hemsley) A. Gray (Asteraceae)) had been very attractive in the east Africa continent and in the most of areas in Asia continent, as well as in Indonesia country of over 14,480 islands as Daun Kembang bulan because of its various biological and chemical properties to sustain human being life on earth. In present paper, our findings are focused on the unique physical characters sustained with a simple theoretical model to explain the interplay quantity of mechanical vibration of 2 main compounds of Tagitinine A (C19H28O7) and Tagitinine C or artemisinic acid (C15H22O2) identified using HPLC in such natural healthy drink produced by our Herbal Blessing standard products in nanotechnology research center and innovative creation (PPNRI) at Pattimura university. Such interesting finding suggests that the interactive vibration only involved about ~23.763 % mass of the total conserved mass