Free Radicals, Antioxidants and Disease

Sharma, Neeti

doi:10.4172/0974-8369.1000214

Cited by 59 publications

(34 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…induced oxidative stress leads to lipid peroxidation and causes cell damage because of its half-life is longer than that of other reactive oxygen species (ROS) and it can easily transformed into a hydroxyl radical, which is one of the most destructive free radical [7,29].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Shinde

Koratkar²,

Sharma³

et al. 2016

Int J Pharm Pharm Sci

Self Cite

View full text Add to dashboard Cite

Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folinciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H induced oxidative stress in HepG2 cell line.2 O2 in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2 Results:The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H +ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels. 2O 2 Conclusion:Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported. treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.

show abstract

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Shinde

Koratkar²,

Sharma³

et al. 2016

Int J Pharm Pharm Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…the only way to produce free radicals; external factors such as smoking, environmental pollutants, radiation, drugs, pesticides, industrial solvents and ozone also contribute to the formation of free radicals (8). Free radicals can be derived from oxygen (Reactive Oxygen Species ROS) or biosynthesized from nitrogen (Reactive Nitrogen Species, RNS) or from sulfur (Reactive Sulfur Species, RSS).…”

Section: Page86mentioning

confidence: 99%

“…RSS are formed from the reaction of ROS and thiols (17,(43)(44)(45)(46). killing micro-organisms and cancer cells by macrophages and cytotoxic lymphocytes and in oxygenases for the generation of prostaglandins and leukotrienes, which have many regulatory functions (8).…”

Section: Page86mentioning

confidence: 99%

The Potential Antioxidant Properties of Date Products: A Concise Update

2018

View full text Add to dashboard Cite

Background: Free radicals are highly unsteady reactive molecules containing one or more free electrons. These molecules can cause organ damage, disease and even death. Phoenix dactylifera L, commonly known as 'date palm' is one of the oldest cultivated plants. Date fruit is considered a source of antioxidants. Objective: This study aims to review the role of antioxidants in scavenging free radicals together with the functional properties of date fruit and their by-products. Results: The review shows that date fruits and their by-products, date syrup and date pits, are good sources of antioxidants. Phenolics, which are considered to be a good source of antioxidants, were found in dates and their by-products along with other antioxidant compounds such as carotenoids and flavonoids. Conclusion: Dates and their by-products have the potential to be used in the production of different types of functional foods due to their antioxidant properties.

show abstract

“…However, tissues, organs, and some of the biological macromolecules will also be damaged by oxidative stress when ROS excesses the receptivity of the human body [3]. Some serious diseases, such as diabetes [4], neurodegenerative disease [5], cancers [6], and other cardiovascular diseases [7] were related to excessive oxidative stress reaction on the human body induced by ROS.…”

Section: Introductionmentioning

confidence: 99%

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Xie

et al. 2019

Foods

View full text Add to dashboard Cite

Mung bean is nutritious and rich in protein (19.5%-33.1%). However, there are few studies on mung bean protein active peptides so the mung bean protein hydrolysates (MBPHs) were investigated for evaluating their ability to clear intracellular reactive oxygen species (ROS) and regulating the ability of antioxidant enzymes on NCTC-1469 cells. Results showed that MBPHs, MBPHs-I (molecular weight < 3 kDa), MBPHs-II (molecular weight between 3 and 10 kDa), and MBPHs-III (molecular weight > 10 kDa) could all improve the survival rate of cells compared with the model group. MBPHs, MBPHs-I, and MBPHs-II could significantly decrease the content of lactate dehydrogenase (LDH) and reduce the generation of malonaldehyde (MDA) at a concentration of 0.4 mg/mL. Regarding the intracellular ROS, the result showed that MBPHs-I significantly reduced the production of ROS (from 58.3% to 26.6%) and had a dose-dependent relationship. In addition, the amino acid analysis showed that MBPHs-I had a balanced amino acid composition. MBPHs-I is rich in lysine but was deficient in cereals. Therefore, the hydrophobic and aromatic amino acids in MBPHs-I were high, which could improve its antioxidant activity. According to the results, MBPHs-I was the best and most potent natural antioxidant and it can contribute to drug development and medical application.

show abstract

Free Radicals, Antioxidants and Disease

Cited by 59 publications

References 77 publications

Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

The Potential Antioxidant Properties of Date Products: A Concise Update

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Contact Info

Product

Resources

About