Frequency of class 1, 2 and 3 integrons in clinical Klebsiella pneumoniae isolates; a systematic review and meta-analysis of cross-sectional studies

The aim: The goal of this study is to identify Uropathogenic E. coli multidrug-resistant bacteria, as well as their genetic profile clade and correlation with dispersion integron. Materials and methods: Five hundred urine samples from UTI patients were collected in Al-Diwaniyah, Iraq. They were then investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Eosin-Methylene blue) to detect Enterobacteriacea, including Escherichia coli, the samples were identified using the Vitec 2 compact system, as well as MIC susceptibility testing to Amikacin, Levofloxacin, Cefepime, Meropenem, Nitrofurantion, and Trimethoprim-sulfamethoxazole for detect multidrug resistance isolates. Multiplex PCR was used to detect three types of integrase gene. Finally, ERIC2-PCR was used to detect the genetic profile of all isolates. Results: From 500 UTI samples, 22 isolates UPEC detected resistance to different class of antibiotic, including: 86.3% to Cefepime, 54.5% - to Trimethoprim-sulfamethoxazole, 31.8% -Levofloxacin, 18.8% - Amikacin, 18.8% - Imipenem, 0% to Nitrofurantion. Twenty of the 22 isolates had various integrase gene classes as: 54.5%, 36.3%, 72.2% for Intl I, Intl II, and Intl III, respectively; while two isolates have no integron. Conclusions: Integron III has a higher incidence and compensates other classes; isolates with triple classes are more virulent and antibiotic resistant. Their genomic profile reveals association with human urine and unique clade of relatives.

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Frequency of class 1, 2 and 3 integrons in clinical Klebsiella pneumoniae isolates; a systematic review and meta-analysis of cross-sectional studies

Cited by 2 publications

References 28 publications

The Global Prevalence of Class 1 Integron and Associated Antibiotic Resistance in Escherichia coli from Patients with Urinary Tract Infections, a Systematic Review and Meta-Analysis

The Global Prevalence of Class 1 Integron and Associated Antibiotic Resistance in Escherichia coli from Patients with Urinary Tract Infections, a Systematic Review and Meta-Analysis

Distribution of Integron Iii and Phylogenic Clade Among MDR Uropathogenic E. Coli From Patient in Al-Diwaniyah City, Iraq

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