Frequency of M-Cadherin-stained Satellite Cells Declines in Human Muscles During Aging

Sajko, Spela; Kubínová, Lucie; Cvetko, Erika; Kreft, Marko; Wernig, A.; Eržen, Ida

doi:10.1177/002215540405200205

Cited by 84 publications

(73 citation statements)

References 44 publications

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“…Our studies demonstrate that M-CAM is expressed on human fetal myogenic cells and its downregulation appears necessary to promote cell fusion. On cytospins of cultured cells, M-CAM was co-expressed with the myoblast-specific markers desmin and M-cadherin, whereas on tissue sections M-CAM colocalized with cells positive for the myogenic markers Pax7 (Seale et al, 2000;Olguin and Olwin, 2004;Zammit et al, 2004) and M-cadherin (Irintchev et al, 1994;Sajko et al, 2004). Collectively, these results suggested that M-CAM may be a novel marker for activated satellite cells, because it appears to be expressed by cells weakly positive for Pax7 (Zammit et al, 2004) and on cultured satellite cells expressing desmin and M-cadherin (Cornelison and Wold, 1997).…”

Section: Discussionmentioning

confidence: 99%

Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion

Cerletti

Molloy

Tomczak

et al. 2006

Journal of Cell Science

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Myoblast fusion is a highly regulated process that is important during muscle development and myofiber repair and is also likely to play a key role in the incorporation of donor cells in myofibers for cell-based therapy. Although several proteins involved in muscle cell fusion in Drosophila are known, less information is available on the regulation of this process in vertebrates, including humans. To identify proteins that are regulated during fusion of human myoblasts, microarray studies were performed on samples obtained from human fetal skeletal muscle of seven individuals. Primary muscle cells were isolated, expanded, induced to fuse in vitro, and gene expression comparisons were performed between myoblasts and early or late myotubes. Among the regulated genes, melanoma cell adhesion molecule (M-CAM) was found to be significantly downregulated during human fetal muscle cell fusion. M-CAM expression was confirmed on activated myoblasts, both in vitro and in vivo, and on myoendothelial cells (M-CAM+ CD31+), which were positive for the myogenic markers desmin and MyoD. Lastly, in vitro functional studies using M-CAM RNA knockdown demonstrated that inhibition of M-CAM expression enhances myoblast fusion. These studies identify M-CAM as a novel marker for myogenic progenitors in human fetal muscle and confirm that downregulation of this protein promotes myoblast fusion.

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Section: Discussionmentioning

confidence: 99%

Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion

Cerletti

Molloy

Tomczak

et al. 2006

Journal of Cell Science

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“…Actually, the number of satellite cells declines with age in humans. 16,17 In addition, the proliferation potential of human muscle satellite cells is limited by cellular senescence induced by progressive telomere shortening. 16,18 When the telomere length becomes less than about 5 kb, the Rb and p53 pathways are activated and culminate in irreversible growth arrest.…”

Section: Discussionmentioning

confidence: 99%

CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential

Kiyono²,

et al. 2011

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In vitro culture systems of human myogenic cells contribute greatly to elucidation of the molecular mechanisms underlying terminal myogenic differentiation and symptoms of neuromuscular diseases. However, human myogenic cells have limited ability to proliferate in culture. We have established an improved immortalization protocol for human myogenic cells derived from healthy and diseased muscles; constitutive expression of mutated cyclin-dependent kinase 4, cyclin D1 and telomerase immortalized human myogenic cells. Normal diploid chromosomes were preserved after immortalization. The immortalized human myogenic cells divided as rapidly as primary human myogenic cells during the early passages, and underwent myogenic, osteogenic and adipogenic differentiation under appropriate culture conditions. The immortalized cells contributed to muscle differentiation upon xenotransplantation to immunodeficient mice under conditions of regeneration following muscle injury. We also succeeded in immortalizing cryopreserved human myogenic cells derived from Leigh disease patients following primary culture. Forced expression of the three genes shortened their cell cycle to o30 h, which is similar to the doubling time of primary cultured human myogenic cells during early passages. The immortalization protocol described here allowed human myogenic cells to recapture high proliferation activity without compromising their differentiation potential and normal diploidy.

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“…The correlation with the cross-sectional fiber area allows processes like muscle growth, pathological events, and ageing to be taken into account (Sajko et al 2004), but it has been suggested that ideally both satellite cells per fiber and satellite cells per number of myonuclei should be used together for a more accurate measurement (Lindstrom and Thornell 2009). …”

Section: Quantification Of Human Satellite Cellsmentioning

confidence: 99%

Are Human and Mouse Satellite Cells Really the Same?

Boldrin¹,

Muntoni²,

Morgan³

2010

J Histochem Cytochem.

117

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S U M M A R Y Satellite cells are quiescent cells located under the basal lamina of skeletal muscle fibers that contribute to muscle growth, maintenance, repair, and regeneration. Mouse satellite cells have been shown to be muscle stem cells that are able to regenerate muscle fibers and self-renew. As human skeletal muscle is also able to regenerate following injury, we assume that the human satellite cell is, like its murine equivalent, a muscle stem cell. In this review, we compare human and mouse satellite cells and highlight their similarities and differences. We discuss gaps in our knowledge of human satellite cells, compared with that of mouse satellite cells, and suggest ways in which we may advance studies on human satellite cells, particularly by finding new markers and attempting to re-create the human satellite cell niche in vitro. (J Histochem Cytochem 58:941-955, 2010)

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Frequency of M-Cadherin-stained Satellite Cells Declines in Human Muscles During Aging

Cited by 84 publications

References 44 publications

Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion

Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion

CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential

Are Human and Mouse Satellite Cells Really the Same?

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