Fructose‐1,6‐bisphosphate aldolase A levels decrease in hair keratinocytes during androgenetic alopecia

Tochio, Takumi; Tanaka, Hiroshi; Nakata, Satoshi; Hosoya, Hiroshi

doi:10.1111/j.1365-4632.2011.05242.x

Cited by 2 publications

(6 citation statements)

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“…Previous studies have shown that the activity of fructose-1,6-bisphosphate aldolase A promoted the metastasis and migration in the lung squamous cell carcinoma [ 24 – 27 ]. We thus performed transwell assays to test the potential role of FBP1 expression on cancer cell invasiveness.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study revealed that FBP1 mRNA was significantly decreased in lung cancer tissues compared with normal tissues [ 21 ]. In addition, the patients with higher level of FBP1 RNA expression have significantly longer disease free survival and overall survival as compared to the lower expression groups [ 25 – 27 ]. In the current study, we determined the underlying mechanism for FBP1 expression suppression and its biological functions in lung cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, we determined the underlying mechanism for FBP1 expression suppression and its biological functions in lung cancer cells. Due to high rate CpG islands located in FBP1 gene promoter region and its possible regulation mechanisms as previously revealed [ 25 – 27 ], we investigated the methylation status of FBP1 promoter region in paired human lung cancer and adjacent normal tissues. Our data showed significantly high rate of methylation in FBP1 promoter in lung cancer tissues verses paired normal tissues, and the detected higher methylation level also corresponds to lower FBP1 expression.…”

Section: Discussionmentioning

confidence: 99%

“…As mentioned above, the detailed mechanisms of antitumor function of FBP1 are still remaining to be unclear; it has elucidated that Snail-G9a-Dnmt1 complex is critical for FBP1 silence, which promoted the interaction of β -catenin and TCF and played an important role in EMT transformation in basal-like breast cancer [ 25 – 27 ]. Recently, Li et al found that FBP1 downregulation could enhance the activity of Wnt/ β -Catenin pathway and increase the level of its downstream targets, including c-Myc and MMP7 in human breast cancer cells [ 25 – 27 ], suggesting that FBP1 might take part in regulating cancer cell migration via Wnt/ β -catenin signaling pathway. On the other hand, FBP1 could decrease glycolytic flux in renal tubular epithelial cells and subsequently inhibits the Warburg effect in lung cancer cells as predicted.…”

Section: Discussionmentioning

confidence: 99%

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Significance of Methylation of FBP1 Gene in Non-Small Cell Lung Cancer

Dong

Sheng

Danying

et al. 2018

BioMed Research International

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Because NSCLC has poor overall prognosis and is frequently diagnosed at later stage, we aimed to seek novel diagnosis biomarkers or therapy target of the disease in this study. Fructose-1,6-bisphosphatase 1 (FBP1) is a rate-limiting enzyme in gluconeogenesis, which was usually lost in NSCLC due to abnormal methylation in promoter DNA sequence. The clinical data indicated that the methylation rate in FBP1 gene promoter was negatively related to the overall survival of the NSCLC patients. DNA methylation transferase inhibitor 5-aza treatment could significantly increase both expression levels of mRNA and protein in A549 cell line. On the other hand, silence of FBP1 in H460 cell line by using specific siRNA against FBP1 dramatically improved the cell proliferation and cell migration according to the date of FACS and transwell assays. All these findings implied the important roles of FBP1 expression in lung cancer development and progression and the potential use of the methylation status detected in FBP1 promoter region as a novel predictor for prognosis and therapeutic target for NSCLC patients.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Significance of Methylation of FBP1 Gene in Non-Small Cell Lung Cancer

Dong

Sheng

Danying

et al. 2018

BioMed Research International

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“…These techniques include cell proliferation assays involving androgen-induced overgrowth and androgen receptor antagonism. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] Twenty-four studies investigated gene expression profiling [18][19][20][21][22][23][24][25][26][27][28] and markers for AGA. [29][30][31][32][33][34][35][36][37][38][39][40][41] Fourteen studies focused on the action of 5-alpha reductase (5AR), which converts the androgen (testosterone) into dihydrotestosterone (DHT) and the inhibition of this enzyme [42][43][44][45][46][47][48][49][50][51]…”

Section: Data Extraction and Synthesis Of Included Studiesmentioning

confidence: 99%

An appraisal of laboratory models of androgenetic alopecia: A systematic review

Ntshingila

Khumalo

Engel

et al. 2021

Skin Health and Disease

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Background: Androgenetic alopecia (AGA) is the most common form of non-scarring alopecia in humans. Several studies have used different laboratory models to study the pathogenesis and interventions for AGA. These study models have proved beneficial and have led to the approval of two drugs. However, the need to build on existing knowledge remains by examining the relevance of study models to the disease. Objective: We sought to appraise laboratory or pre-clinical models of AGA. Method: We searched through databases (PubMed, ScienceDirect, Web of Science, World CAT, Scopus and Google Scholar) for articles on AGArelated studies from 1942 to March 2019 with a focus on study models. Results: The search rendered 101 studies after screening and deduplication. Several studies (70) used in vitro models, mostly consisting of twodimensional monolayer cells for experiments involving the characterization of androgen and 5-alpha reductase (5AR) and inhibition thereof, the effects of dihydrotestosterone (DHT) and biomarker(s) of AGA. Twenty-seven studies used in vivo models of mice and monkeys to investigate DHT synthesis, the expression and inhibition of 5AR and hair growth. Only four studies used AGA-related or healthy excisional/punch biopsy explants as ex vivo models to study the action of 5AR inhibitors and AGA-associated genes. No study used three-dimensional [3-D] organoids or organotypic human skin culture models. Conclusion:We recommend clinically relevant laboratory models like human or patient-derived 3-D organoids or organotypic skin in AGA-related studies. These models are closer to human scalp tissue and minimize the use of laboratory animals and could ultimately facilitate novel therapeutics. | BACKGROUNDAndrogenetic alopecia (AGA) is the most common form of non-scarring hair loss in humans. [1][2][3] The prevalence of AGA varies between races and ethnicities. 1 This disparity is attributed to the different methods of measuring prevalence, making it difficult to compare studies. 1,2 Nonetheless, about 50% of men of European descent are affected by the age of 50 years; this proportion increases to 90% with age. 1,4,5 Furthermore, AGA is estimated to affect about 19% of women of European ancestry, while the prevalence and severity of AGA are considered low in Asian and African men. 1,4,6 In the early 1940s, Hamilton proved that genetic predisposition and male hormone stimulation are prerequisites in AGA development. 7 After this discovery, several AGA models were created to delineate the pathophysiology and evaluate the effectiveness of novel therapeutics using both laboratory (in vitro, in vivo and ex vivo) and non-laboratory models. These models,This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fructose‐1,6‐bisphosphate aldolase A levels decrease in hair keratinocytes during androgenetic alopecia

Cited by 2 publications

References 10 publications

Significance of Methylation of FBP1 Gene in Non-Small Cell Lung Cancer

Significance of Methylation of FBP1 Gene in Non-Small Cell Lung Cancer

An appraisal of laboratory models of androgenetic alopecia: A systematic review

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