Fructose compared with glucose is more a potent glycoxidation agent in vitro, but not under carbohydrate-induced stress in vivo: potential role of antioxidant and antiglycation enzymes

Semchyshyn, Halyna M.; Międzobrodzki, Jacek; Bayliak, Maria M.; Lozinska, Liudmyla; Homza, Bohdana V.

doi:10.1016/j.carres.2013.11.015

Cited by 41 publications

(31 citation statements)

References 67 publications

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“…Likewise, in broccoli, an increase in sugar contents has been proposed as a way to mask the bitter taste of sulfur-containing glucosinolates and promote consumption (Schonhof et al, 2004), and it also plays an important role in the postharvest senescence of the vegetable (King and Morris, 1994). Furthermore, both glucose and fructose are glycoxidating agents and are capable of producing reactive compounds (Semchyshyn et al, 2014). The accumulation and concentration of sugars is dependent on several factors such as genotypes, type of tissues, developmental stages, and growing seasons (Hodges et al, 2006;Hounsome et al, 2008;Lee et al, 1970;Nunes, 2008;VandenLangenberg et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variation in Contents of Sugars in Different Parts of Broccoli

Bhandari¹,

Kwak²

2015

HST

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Seasonal variation in the contents of sugars (fructose, glucose, and sucrose) in the floret, leaf, and stem of broccoli were studied in ten commercial broccoli cultivars. Plants were grown in the spring and fall seasons in 2011. In both seasons, glucose was the major constituent, comprising about 50% of the total sugar content in the floret and leaf tissue of most cultivars, whereas the broccoli stem showed an unusual pattern of accumulation. Sucrose exhibited greater cultivar dependency as well as seasonal variation compared to fructose and glucose in floret and leaf tissues. The floret tissue had a higher total content of sugar in the spring compared to the fall due to an increase in glucose and fructose. However, most of the leaf and stem tissues of broccoli had a higher total sugar content in the fall compared to the spring. Furthermore, stem and leaf tissues possessed a relatively higher total sugar content compared to floret tissue in both seasons. 'Grandeur' broccoli contained a significantly greater amount of total sugar in both floret and leaf tissues in both seasons, whereas 'YuDoRi No.1' broccoli exhibited the highest total content of sugar in stem tissue. At overall, the results showed significant influences of genotype, plant part and growing season on sugar content in broccoli.

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Section: Introductionmentioning

confidence: 99%

Seasonal Variation in Contents of Sugars in Different Parts of Broccoli

Bhandari¹,

Kwak²

2015

HST

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“…Alternatively, FRU may also generate ROS via non‐enzymatic reactions (reviewed by Semchyshyn, ). Recently, FRU autoxidation was shown to produce more ROS and carbonyl species in vitro than those produced by GLU autoxidation (Semchyshyn et al, ). Both FRU and GLU form furanose and pyranose structures (Cocinero et al, ), but the proportions of the acyclic forms of FRU are much greater (∼700‐fold) than those of GLU (Hayward and Angyal, ).…”

Section: Discussionmentioning

confidence: 99%

Fructose sensitizes Jurkat cells oxidative stress‐induced apoptosis via caspase‐dependent and caspase‐independent mechanisms

Diaz-Aguirre

Vélez-Pardo

Jiménez-Del-Río

2016

Cell Biology International

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Whether fructose (FRU), as the sole energy source, confers a metabolic advantage on cancer cells against noxious stimuli is unknown. The aim of this study was to evaluate the effects of low (11 mM), moderate (25 mM), and high (55 mM) FRU concentrations alone or in combination with rotenone (ROT) or doxorubicin (DOX) in Jurkat cells, an acute lymphoblastic leukemia cell model. Glucose (GLU) was used as a control. Using different cell analysis techniques, we demonstrated that FRU was predominantly metabolized via oxidative phosphorylation (∼95%) (i.e., lactate production was reduced >120-fold), resulting in endogenous oxidative stress-induced conditions. The cells were characterized by generation of O (43%)/ H O (40%) and activation of NF-κB (∼95-fold increase, fi), c-Jun-N terminal kinase (JNK), p53 (40-fi), and c-Jun (9-fi). In addition, we observed a loss of ΔΨ (10%), activation of caspase-3 (50-fi) and apoptosis-inducing factor (AIF, 2-fi), and condensation and fragmentation of the nuclei [20% by acridine orange/ethidium bromide/Hoechst (AO/EB/H) staining, 15% by flow cytometry] compared to those of GLU 11 at 24 h. Although DOX killed Jurkat cells independent of sugar content in the culture medium, leukemic cells in low, but not high, FRU were extremely sensitive to ROT. Taken together, our findings suggest that Jurkat cells are more susceptible to cell death if forced to shift from GLU metabolism (i.e., aerobic glycolysis) to FRU metabolism (i.e., oxidative phosphorylation) after treatment with mitochondria-targeting molecules. These observations may help elucidate the cell death mechanism of leukemic cells cultured in FRU.

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“…Another possibility is that the damage due to anoxia and hyperglycemia is specifically due to the production of advanced glycation products but this is not likely since fructose (Semchyshyn et al, 2014) is more potent that glucose for forming glycation products.…”

Section: Sites Of Anoxic Alterations In Metabolic Pathwaysmentioning

confidence: 97%