FT-IR analysis of BSA fouled on ultrafiltration and microfiltration membranes

Maruyama, Takaharu

doi:10.1016/s0376-7388(01)00502-6

Cited by 235 publications

(79 citation statements)

References 19 publications

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“…4). Generally, in water, bands between 1,654 and 1,658 cm −1 are assigned to α-helices, as observed for the reference standard, BSA, which has a predominantly α-helix structure (19).…”

Section: Spectroscopic Analysesmentioning

confidence: 71%

Physicochemical Characterization of Complex Drug Substances: Evaluation of Structural Similarities and Differences of Protamine Sulfate from Various Sources

Awotwe-Otoo

Agarabi

Keire³

et al. 2012

AAPS J

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Abstract. The purpose of this study was to characterize and evaluate differences of protamine sulfate, a highly basic peptide drug, obtained from five different sources, using orthogonal thermal and spectroscopic analytical methods. Thermogravimetric analysis and modulated differential scanning calorimetry showed that all five protamine sulfate samples had different moisture contents and glass transition and melting temperatures when temperature was modulated from 25 to 270°C. Protamine sulfate from source III had the highest residual moisture content (4.7±0.2%) at 105°C, resulting in the lowest glass transition (109.7°C) and melting (184.2°C) temperatures compared with the other four sources. By Fourier-transform infrared (FTIR) spectroscopy, the five sources of protamine sulfate had indistinguishable spectra, and the spectra were consistent with a predominantly random coil conformation in solution and a minor population in a β-sheet conformation (~12%). Circular dichroism spectropolarimetry confirmed the FTIR results with prominent minima at 206 nm observed for all five sources. Finally, proton ( 1 H) nuclear magnetic resonance spectroscopy showed that all five protamine sulfate sources had identical spectra with backbone amide chemical shifts between 8.20 and 8.80 ppm, consistent with proteins with predominantly random coil conformation. In conclusion, thermal analyses showed differences in the thermal behavior of the five sources of protamine sulfate, while spectroscopic analyses showed the samples had a predominantly random coil conformation with a small amount of β-sheet present.

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“…4). Generally, in water, bands between 1,654 and 1,658 cm −1 are assigned to α-helices, as observed for the reference standard, BSA, which has a predominantly α-helix structure (19).…”

Section: Spectroscopic Analysesmentioning

confidence: 71%

Physicochemical Characterization of Complex Drug Substances: Evaluation of Structural Similarities and Differences of Protamine Sulfate from Various Sources

Awotwe-Otoo

Agarabi

Keire³

et al. 2012

AAPS J

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“…FT-IR analysis is used to characterize the major functional groups of organic matters and to predict the major components (Maruyama et al, 2001). EPS extracted from biomass were dried at 70°C for 48 h, and analyzed by a FT-IR spectrometer (Nicolet 5700, Thermo Electron Corporation, USA) in order to obtain the organic substance information in EPS.…”

Section: Fourier Transform Infrared (Ft-ir) Spectroscopymentioning

confidence: 99%

Spatial distributions of biofilm properties and flow pattern in NiiMi process

Zhang¹,

Xing²,

Wu³

et al. 2011

Bioresource Technology

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“…adsorption of low molecular weight substances at the pore walls, cake built-up at the surface of the membrane and mechanical pore plugging. Some molecules, such as proteins, polysaccharides, humic acids and FFA, are reported to cause drastic flux reductions during filtration processes although their size is much smaller than the pore diameter [16][17][18][19][20][21]. This internal fouling is attributed to pore narrowing as a result of the adsorption of these compounds.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive fouling of inorganic membranes during microfiltration of vegetable oils

Hafidi

Pioch

Ajana

2003

Euro J Lipid Sci & Tech

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a The objective of the present paper is to contribute to a better understanding of the fouling phenomenon occurring during microfiltration of crude vegetable oil. To assess the role some oil components play in the fouling, the latter was monitored by the evolution of fluxes during the microfiltration of different model oils. Resistances to mass transfer were discussed to estimate the contribution of different phenomena and to compare hydrophilic and hydrophobic membrane materials. A drastic flux decline was obtained when filtering crude sunflower oil through different types of alumina membranes. Purified sunflower oil, with or without added free fatty acids, did not show any important fouling during crossflow microfiltration, suggesting that neither triglycerides nor free fatty acids are involved in the phenomenon to a large extent. By way of contrast, addition of phospholipids to the purified oil caused a drastic flux drop similar to that observed for the crude oil. The presence of small amounts of phospholipids increased the resistance to about 60 to 130% compared to that of the purified oil. The reason is most likely a fast adsorption occurring in the first instants during membrane wetting. Overall the fouling phenomenon is probably due to the fact that the phospholipids form supramolecular structures at the surface of the membrane.

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FT-IR analysis of BSA fouled on ultrafiltration and microfiltration membranes

Cited by 235 publications

References 19 publications

Physicochemical Characterization of Complex Drug Substances: Evaluation of Structural Similarities and Differences of Protamine Sulfate from Various Sources

Physicochemical Characterization of Complex Drug Substances: Evaluation of Structural Similarities and Differences of Protamine Sulfate from Various Sources

Spatial distributions of biofilm properties and flow pattern in NiiMi process

Adsorptive fouling of inorganic membranes during microfiltration of vegetable oils

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