FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

Abstract

Introduction: Patients with diabetes mellitus exhibit a delay in the lesion repair process. The active components of Cenostigma macrophyllum may represent a viable alternative to facilitate the recovery of these lesions. The aim of this study was to evaluate the effects of emulsion oil-water Cenostigma macrophyllum in the repair process of lesions in rats with induced diabetes. Methods: 63 male rats (Wistar, 200-250 g body weight, 30-40 days old) were distributed into the following groups: control (C), diabetic (D) and diabetic treated with Cenostigma macrophyllum (P), subdivided based on the experimental times, days 7, 14 and 28, with 21 animals per main group. Diabetes mellitus (DM) was induced by administration of streptozotocin (50 mg/kg via penile vein and 12-h fasting) and confirmed at day 21 (glycemic index > 240 mg/dL). In the animals of group P, 0.5 ml of the oil-water emulsion obtained from the plant seed was used. The samples were removed and hemisectioned, and one portion was used for the quantitative histological analysis of collagen using Masson's trichrome staining, while another portion was analyzed by FT-Raman spectroscopy. Results: A higher percentage area of the volume of collagen fibers was observed for the experimental time Day 14 in group P compared with group D (p < 0.001). Regarding the ratio of areas of the amides I (1700-1600 cm -1 ) and III (1245-1345 cm -1 ), the groups D and P show the opposite behavior. Conclusion: Cenostigma macrophyllum accelerated the repair process in skin of diabetic ratsfor14 days.

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

“…3,10 The effect of the treatment used to accelerate tissue repair can be assessed by quantifying and differentiating the collagenous fibers in the dermis at various stages of the healing process. [5][6][7]40 Thus, the results of this study show the applicability of FT-IR for the analysis and monitoring of the process of tissue repair in the experimental model using rats. ).…”

“…The first six principal components (PC1-PC6) were used in the linear discriminant analyses with the aid of the cross validation method. 6,[29][30][31] The linear discriminant analysis was carried out for each group, as a function of experimental time, to differentiate the separation in the repair phases. The second derivative of the spectra was used to show change in the bands according to the treatment.…”

“…However, new analytical techniques have been studied for a better understanding of tissue repair process. 4,6,7 Among the optical spectroscopic techniques, Fourier transform infrared spectroscopy (FT-IR) stands out for its ability to identify biological tissue components through the characterization of vibrational modes of molecular radicals. 8 It produces spectral images of histological sections and identifies biomolecular changes in tissues by studying the variance of the vibrational bands of biomolecules.…”