FTICR-MS and LC-UV/MS-SPE-NMR Applications for the Rapid Dereplication of a Crude Extract from the Sponge <i>Ianthella flabelliformis</i>

Motti, Cherie A.; Freckelton, Marnie L.; Tapiolas, Dianne M.; Willis, Richard H.

doi:10.1021/np800562m

Cited by 41 publications

(36 citation statements)

References 23 publications

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“…These same peaks were also detected in the purple morphotype along with two distinct isotope envelopes at m/z 795 and m/z 811, identified based on their accurate mass data as 19-hydroxy-araplysillin-I N 20 -sulfamate (1) and araplysillin-I N 20 -sulfamate (2), respectively (Motti et al, 2009). The metabolite profile for the anomalous sample P8 was found to resemble those of the yellow samples.…”

Section: Chemometric Analysis Of Fticr-ms Datamentioning

confidence: 60%

“…The presence of chemically and microbially distinct profiles in I. basta has further ecological implications as the araplysillin and bastadin classes of compounds are known to have strong biological activity. Previous studies examining the PPDK inhibitory activity of marine organisms (Doyle et al, 2005) showed that only the crude extract of the purple morphotype of I. basta was active; this activity was subsequently attributed to compounds 1 and 2 (Motti et al, 2009). It is also well documented that I. basta contains carotenoids, ingested and partially metabolised by the sponge (Hertzberg et al, 1989), and that the sponge tissue changes colour within minutes of collection from yellow and purple to a blackish purple due to pigment oxidation.…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 1 g wet weight of the remaining sponge tissue (8 yellow morphotypes and 17 purple morphotypes) was immediately immersed in 10 ml HPLC grade methanol (MeOH, Mallinckrodt), steeped overnight and stored at 4°C prior to mass spectral analysis. MeOH was the solvent of choice for the extraction based on previous chromatography analyses which yielded an abundance of chemistry (Motti et al, 2009).…”

Section: Specimen Collectionmentioning

confidence: 99%

“…A 10 ll aliquot of each MeOH extract was diluted into 500 ll MeOH (0.5 mg/ml) ready for mass spectral analysis. Hydrobiologia (2012) 687:191-203 193 were performed on each extract using an unmodified Bruker BioAPEX 47e mass spectrometer equipped with an Analytica of Branford model 103426 (Branford, CT) electrospray ionisation (ESI) source in negative mode (cylinder = 1.9 kV, source = 4.0 kV and end cap = 3.5 kV, capillary exit = -160 V and skimmer potential = -10 V) (Motti et al, 2009). Direct infusion of each extract was carried out using a Cole Palmer 74900 syringe pump at a rate of 150 ll/h and the instrument externally calibrated using a methanolic solution of CF 3 COONa (0.1 mg/ml MeOH).…”

Section: Fticr-ms Analysismentioning

confidence: 99%

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Qualitative variation in colour morphotypes of Ianthella basta (Porifera: Verongida)

et al. 2011

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Natural populations of marine invertebrates often exhibit measureable morphologic variation resulting in taxonomic confusion. This potentially has severe consequences for experimental design and data management. Species of the sponge genus Ianthella embody a number of different morphologies and a diverse range of secondary metabolites. Among them, Ianthella basta (Pallas, 1776), a common sponge in Papua New Guinea and the Great Barrier Reef (GBR), exhibits two dominant colour morphotypes: yellow and purple. Specimens collected from Orpheus Island on the GBR were investigated using phylogenetic (CO1, ITS-2 sequence analysis), chemical (mass spectrometry) and microbial (DGGE and 16S rRNA clone library) techniques in an effort to fully characterise the two colour morphs. Phylogenetic analyses indicated sharp genetic discontinuities within I. basta sensu lato independent of colour variation. The two morphotypes did, however, correspond to distinct DGGE profiles largely due to the presence of additional bands in the purple morpho-group. Further comparison of the microbial communities by 16S rRNA gene sequencing revealed that whilst both colour morphs were dominated by only two bacterial symbionts (residing within the Gamma and Alphaproteobacteria), the purple morph also contained minor representatives of the Cyanobacteria, Chloroflexi and Verrucomicrobia. Untargeted metabolic profiling by Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) indicated two distinct clusters corresponding to the different sponge colours. A clear association was found between the araplysillin class of compounds and the purple morphotype of I. basta, indicating the utility of a metabolomic approach to assess differences between colour morphs. These results have important implications for ecological investigations in sponges and other invertebrate taxa whose morphology is fundamentally dynamic, stressing the need for precise taxonomic, chemical and microbial descriptions.

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Section: Chemometric Analysis Of Fticr-ms Datamentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Specimen Collectionmentioning

confidence: 99%

Section: Fticr-ms Analysismentioning

confidence: 99%

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Qualitative variation in colour morphotypes of Ianthella basta (Porifera: Verongida)

et al. 2011

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“…Data were acquired and processed using Xcalibur 1.4 SR1 software (Thermo Finnigan, San Jose, CA). For detailed information on the instrumentation please refer to Supporting Information accompanying Motti et al (2009).…”

Section: Hplc-ft-msmentioning

confidence: 99%

Identification of peptides from sinus gland extracts in the red swampcrayfish Procambarus clarkii by mass spectrometry

Zhou

2016

IJAR

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Crustacean sinus gland (SG) is a well-defined neuroendocrine organ that controls the secretion of various neuropeptides which regulate many physiological activities. The red swamp crayfish Procambarus clarkii is a decapod crustacean with both high economic and scientific importance in China. To facilitate physiological investigations of SG peptide/hormone function in this species, we have been employed tissue extract fractionation by HPLC to obtain a complete description of neuropeptidome and used Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS) to detect the peptidome present in its organ. In total, 48 peptide sequences belonging to several known neuropeptide families including crustacean cardioactive peptide, CHH precursor-related peptides (CPRPs), orcokinins and pigment dispersing hormones were identified. Among these 48 sequences, 26 are novel peptides and 22 are previously identified. Overall, the results give a stimulus for future physiological studies of SG neuropeptides in P. clarkii and other crustaceans.

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Hyphenation of NMR with Other Analytical Separation Techniques

Elipe

Victoria²

2011

LC‐NMR and Other Hyphenated NMR Techniques

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FTICR-MS and LC-UV/MS-SPE-NMR Applications for the Rapid Dereplication of a Crude Extract from the Sponge Ianthella flabelliformis

Cited by 41 publications

References 23 publications

Qualitative variation in colour morphotypes of Ianthella basta (Porifera: Verongida)

Qualitative variation in colour morphotypes of Ianthella basta (Porifera: Verongida)

Identification of peptides from sinus gland extracts in the red swampcrayfish Procambarus clarkii by mass spectrometry

Hyphenation of NMR with Other Analytical Separation Techniques

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