FtsK and SpoIIIE, coordinators of chromosome segregation and envelope remodeling in bacteria

Chan, Helena; Mohamed, A. M.; Grainge, Ian; Rodrigues, Christopher D. A.

doi:10.1016/j.tim.2021.10.002

Cited by 22 publications

(18 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RlpA is an accessory component of the E. coli divisome, as evidenced by its protein–protein interaction with FtsK 59 . FtsK is a large, bitopic protein that acts at the late stage of septum formation as both a DNA translocase and as a checkpoint for final septal closure 59 , 60 , 79 , 80 . However, the role of RlpA in the E. coli divisome is structural and is not catalytic, as a result of a point mutation at the position of the catalytic aspartate (which is present in P. aeruginosa RlpA), and as evidenced by the absence of a phenotype upon its genetic deletion 38 , 49 .…”

Section: Discussionmentioning

confidence: 99%

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

et al. 2022

View full text Add to dashboard Cite

The protein networks of cell-wall-biosynthesis assemblies are largely unknown. A key class of enzymes in these assemblies is the lytic transglycosylases (LTs), of which eleven exist in P. aeruginosa. We have undertaken a pulldown strategy in conjunction with mass-spectrometry-based proteomics to identify the putative binding partners for the eleven LTs of P. aeruginosa. A total of 71 putative binding partners were identified for the eleven LTs. A systematic assessment of the binding partners of the rare lipoprotein A (RlpA), one of the pseudomonal LTs, was made. This 37-kDa lipoprotein is involved in bacterial daughter-cell separation by an unknown process. RlpA participates in both the multi-protein and multi-enzyme divisome and elongasome assemblies. We reveal an extensive protein-interaction network for RlpA involving at least 19 proteins. Their kinetic parameters for interaction with RlpA were assessed by microscale thermophoresis, surface-plasmon resonance, and isothermal-titration calorimetry. Notable RlpA binding partners include PBP1b, PBP4, and SltB1. Elucidation of the protein-interaction networks for each of the LTs, and specifically for RlpA, opens opportunities for the study of their roles in the complex protein assemblies intimately involved with the cell wall as a structural edifice critical for bacterial survival.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Furthermore, DNA translocases coordinate chromosome segregation with septum closure by clearing the midcell of chromosomal DNA by actively pumping it across the division septum (see H. Chan et al, 2022 for a recent review). S. aureus is known to encode two putative DNA translocases, SpoIIIE and FtsK, and it has been shown that the cells require one of these proteins for normal chromosome segregation (Veiga & Pinho, 2017).…”

Section: Chromosome Replication and Segregationmentioning

confidence: 99%

The cell cycle of Staphylococcus aureus: An updated review

et al. 2022

View full text Add to dashboard Cite

As bacteria proliferate, DNA replication, chromosome segregation, cell wall synthesis, and cytokinesis occur concomitantly and need to be tightly regulated and coordinated. Although these cell cycle processes have been studied for decades, several mechanisms remain elusive, specifically in coccus‐shaped cells such as Staphylococcus aureus . In recent years, major progress has been made in our understanding of how staphylococci divide, including new, fundamental insights into the mechanisms of cell wall synthesis and division site selection. Furthermore, several novel proteins and mechanisms involved in the regulation of replication initiation or progression of the cell cycle have been identified and partially characterized. In this review, we will summarize our current understanding of the cell cycle processes in the spheroid model bacterium S. aureus , with a focus on recent advances in the understanding of how these processes are regulated.

show abstract

“…Membrane bulges were also observed earlier in S. aureus ΔftsK/ΔspoIIIE mutants ( 47 ). Recently, the roles of FtsK/SpoIIIE have been suggested in envelope remodeling in bacteria ( 48 ). All of this indicates the role of drFtsK in envelope reorganization during cell division and in mutants, where the lack of this function might have caused the delay in the growth rate ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

FtsK, a DNA Motor Protein, Coordinates the Genome Segregation and Early Cell Division Processes in Deinococcus radiodurans

Mishra

Misra

Kota

2022

mBio

View full text Add to dashboard Cite

show abstract

FtsK and SpoIIIE, coordinators of chromosome segregation and envelope remodeling in bacteria

Cited by 22 publications

References 79 publications

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

The cell cycle of Staphylococcus aureus: An updated review

FtsK, a DNA Motor Protein, Coordinates the Genome Segregation and Early Cell Division Processes in Deinococcus radiodurans

Contact Info

Product

Resources

About