Full inhibition of enzymatic browning in the presence of thiol-functionalised silica nanomaterial

Muñoz-Pina, Sara; Ros‐Lis, José V.; Argüelles, Ángel; Coll, Carmen; Martínez‐Máñez, Ramón; Andrés, Ana

doi:10.1016/j.foodchem.2017.08.059

Cited by 28 publications

(19 citation statements)

References 34 publications

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“…Silica (SiO 2 ) particles are used widely in the fields of drug delivery [68,69], food industry [70,71], catalytic [72], sensor [73], biochemical [74], etc. [75,76] because of their special porous structure, biocompatibility, colloidal stability, low cost, and easy preparation.…”

Section: Polyaniline-coated Core-shell Structured Microspheresmentioning

confidence: 99%

Polyaniline Coated Core-Shell Typed Stimuli-Responsive Microspheres and Their Electrorheology

Dong¹,

Han²,

Choi³

2018

Polymers

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Functional core-shell-structured particles have attracted considerable attention recently. This paper reviews the synthetic methods and morphologies of various electro-stimuli responsive polyaniline (PANI)-coated core-shell-type microspheres, including PANI-coated Fe 3 O 4 , SiO 2 , Fe 2 O 3 , TiO 2 , poly(methyl methacrylate), poly(glycidyl methacrylate), and polystyrene along with their electrorheological (ER) characteristics when prepared by dispersing these particles in an insulating medium. In addition to the various rheological characteristics and their analysis, such as shear stress and yield stress of their ER fluids, this paper summarizes some of the mechanisms proposed for ER fluids to further understand the responses of ER fluids to an externally applied electric field.

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Section: Polyaniline-coated Core-shell Structured Microspheresmentioning

confidence: 99%

Polyaniline Coated Core-Shell Typed Stimuli-Responsive Microspheres and Their Electrorheology

Dong¹,

Han²,

Choi³

2018

Polymers

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“…This immobilization is accompanied by a variation in activity, that can reach a complete inhibition in the case of materials functionalized with thiols. Thus, the interaction of biological molecules with nanomaterials is not innocent and can be used to modulate their activity [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Use of Silica Based Materials as Modulators of the Lipase Catalyzed Hydrolysis of Fats under Simulated Duodenal Conditions

et al. 2020

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The effect of silica materials and their functionalization in the lipase catalyzed fat hydrolysis has been scarcely studied. Fifteen silica materials were prepared and their effect on the fat hydrolysis was measured, under simulated duodenal conditions, using the pH-stat method. The materials are composed of the combination of three supports (Stöber massive silica nanoparticles, Stöber mesoporous nanoparticles and UVM-7) and four surface functionalizations (methyl, trimethyl, propyl and octyl). In addition, the non-functionalized materials were tested. The functional groups were selected to offer a hydrophobic character to the material improving the interaction with the fat globules and the lipase. The materials are able to modulate the lipase activity and their effect depending on the support topology and the organic covering, being able to increase or reduce the fat hydrolysis. Depending of the material, relative fat hydrolysis rates of 75 to 140% in comparison with absence of the material were obtained. The results were analyzed by Partial Least Square Regression and suggest that the alkyl modified mesopores are able to improve the fat hydrolysis, by contrast the non-porous nanoparticles and the textural pores tend to induce inhibition. The effects are more pronounced for materials containing long alkyl chains and/or in absence of taurodeoxycholate.

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“…Polyphenol oxidase (PPO) is a general natural multi-copper oxidoreductase and plays significant roles in the metabolic processes of many fruits and vegetables (Mayer, 2006). Many studies have shown that enzymatic browning is an undesirable phenomenon during storage and processing of plants food and is mainly initiated by the action of PPO (Muñoz-Pina et al, 2018). PPO is released from the cytoplasm when the yam is damaged by inappropriate handling, and it catalyses polyphenols into quinones under aerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of polyphenol oxidase (PPO) from water yam (Dioscorea alata)

Peng

et al. 2019

CyTA - Journal of Food

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Polyphenol oxidase (PPO) is responsible for the enzymatic browning of fruits and vegetables, and thus has negative effects on the quality of food. In this study, extraction and purification of PPO from water yam was performed successfully, and the characteristics of PPO and inhibitors of enzyme activity were analysed. The results showed that PPO activity improved by 4.58-fold through the DEAE-Sepharose and Superdex G-75 purification technique, which reached electrophoretic purity. The enzyme molecular weight was estimated to be 32 kDa. The optimum pH and temperature values of PPO were 6.0 and 35°C, respectively, and it was not thermostable at higher temperatures. PPO had the highest substrate affinity towards catechol (Km = 27.82 mmol/L and Vmax = 1.464 △A/ min). The most effective inhibitor was L-cysteine followed by ascorbic acid and sodium sulfite. Purificación y caracterización de la polifenol oxidasa (PPO) obtenida del ñame de agua (Dioscorea alata) RESUMEN La polifenol oxidasa (PPO) es responsable del pardeamiento enzimático de frutas y verduras; por lo tanto, tiene efectos negativos en la calidad de los alimentos. En el presente estudio se extrajo y se purificó con éxito la PPO proveniente del ñame de agua, y se analizaron sus características y los inhibidores de la actividad enzimática. En este sentido, los resultados indicaron que la actividad de la PPO mejoró 4.58 veces al emplear la técnica de purificación DEAE-Sepharose y Superdex G-75, que alcanzó la pureza electroforética. El peso molecular de la enzima se estimó en 32 kDa. Los valores óptimos de pH y temperatura de la PPO fueron 6.0 y 35°C, respectivamente, constatándose que no era termoestable a temperaturas más altas. La PPO mostró mayor afinidad de sustrato hacia el catecol (Km = 27.82 mmol/L y Vmax = 1.464 △A/min). Su inhibidor más efectivo fue la L-cisteína, seguida por el ácido ascórbico y el sulfito de sodio.

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Full inhibition of enzymatic browning in the presence of thiol-functionalised silica nanomaterial

Cited by 28 publications

References 34 publications

Polyaniline Coated Core-Shell Typed Stimuli-Responsive Microspheres and Their Electrorheology

Polyaniline Coated Core-Shell Typed Stimuli-Responsive Microspheres and Their Electrorheology

Use of Silica Based Materials as Modulators of the Lipase Catalyzed Hydrolysis of Fats under Simulated Duodenal Conditions

Purification and characterization of polyphenol oxidase (PPO) from water yam (Dioscorea alata)

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