Full-Length Genome Sequence of Japanese Encephalitis Virus Strain FC792, Isolated from Guangxi, China

Lu, Bingyue; Qin, Yibin; Li, Bin; He, Ying; Duan, Qunpeng; Liang, Jiaxing; Chen, Zhongwei; Su, Qianlian; Bi, Bingfen; Zhao, Wei

doi:10.1128/genomea.01054-17

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…Inter-genotypic recombination was also reported by Zhao X., et al (2017), who demonstrated that the virus from fecal isolation (CH-ZXY1) recombined genotypes 1 and 5. Most studies reported that the junction between P1 and P2 was frequency responsiveness for the breakpoint of the HPeV genome 34 (lower panel of Fig. 3 A), and this corresponds with our finding (upper panel of Fig.…”

Section: Discussionsupporting

confidence: 92%

Phylogenetic molecular evolution and recombination analysis of complete genome of human parechovirus in Thailand

Chieochansin

Puenpa

Poovorawan

2021

Sci Rep

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Human parechovirus (HPeV), which is a member of the Picornavirus group of viruses, is a pathogen that is reported to be associated with manifestations that include respiratory tract involvement, gastroenteritis, sepsis-like symptom, and central nervous system complication. Until now, nineteen genotypes have been identified. The lack of proofreading property of viral RNA-dependent RNA polymerase (RdRp) together with recombination among the intra- and inter-genotypes of the virus results in high diversity. However, data specific to the molecular evolutionary perspective of the complete genome of HPeV remains limited. This study aimed to analyze the phylogenetic, molecular evolution, and recombination characteristics of the complete genome of HPeV strains isolated in Thailand during 2009–2012. Fifty-eight samples that were previously confirmed to be HPeV positive and then evaluated for genotyping were subjected to complete genome amplification to generate ten overlapping PCR fragments using a set of in-house designed primers. The same position of the viral genome was read in triplicate using direct Sanger sequencing. All samples were classified into the same previously defined genotypes in both whole-genome and VP1 phylogenic tree. However, sample B1091/HPeV14/2011 exhibited discordant grouping between whole-genome and VP1 on the phylogenetic tree. Bootscan analysis revealed that B1091/HPeV14/2011 inherited from two genotypic viruses, including VP1 from HPeV14, and the rest of the genome from HPeV1B. The results of this study provide important insights into the molecular evolution of and recombination in the viral genome of HPeV that will improve and accelerate our ability to develop treatment and prophylactic strategies in the future.

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Section: Discussionsupporting

confidence: 92%

Phylogenetic molecular evolution and recombination analysis of complete genome of human parechovirus in Thailand

Chieochansin

Puenpa

Poovorawan

2021

Sci Rep

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“…Few seroprevalence data were reported for China, even though the virus was detected in pigs by RT-PCR on several occasions [64,68,69,70,71]. However, in Tibet, three studies reported IgM prevalence levels using capture ELISA ranging from 5% to 33% of the sampled pigs.…”

Section: Resultsmentioning

confidence: 99%

“…However, there are now several studies showing the replacement of GIII by GI that could negatively modify the effectiveness of current vaccines [113,189,190]. JEV was indeed detected in vaccinated populations in aborted fetuses and stillborn piglets in China [68,70]. The authors warned against a potential lack of effectiveness of the vaccine and suggested that the safety of the SA14-14-2 strain belonging to GIII, which is used for vaccine development in pigs, should be reassessed;The currently available vaccines do not confer full protection against the emerging JEV GV strain [191,192].…”

Section: Discussionmentioning

confidence: 99%

How Central Is the Domestic Pig in the Epidemiological Cycle of Japanese Encephalitis Virus? A Review of Scientific Evidence and Implications for Disease Control

Ladreyt

Durand

Dussart

et al. 2019

Viruses

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Despite the existence of human vaccines, Japanese encephalitis (JE) remains the leading cause of human encephalitis in Asia. Pigs are described as the main amplifying host, but their role in JE epidemiology needs to be reassessed in order to identify and implement efficient control strategies, for both human and animal health. We aimed to provide a systematic review of publications linked to JE in swine, in terms of both individual and population characteristics of JE virus (JEV) infection and circulation, as well as observed epidemiological patterns. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement to select and analyze relevant articles from the Scopus database, 127 of which were included in the review. Pigs are central, but the implication of secondary hosts cannot be ruled out and should be further investigated. Although human vaccination cannot eradicate the virus, it is clearly the most important means of preventing human disease. However, a better understanding of the actual involvement of domestic pigs as well as other potential JEV hosts in different JEV epidemiological cycles and patterns could help to identify additional/complementary control measures, either by targeting pigs or not, and in some specific epidemiological contexts, contribute to reduce virus circulation and protect humans from JEV infection.

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“…The viral nucleic acid and capsid protein form a nucleocapsid that is surrounded by a host-derived lipid bilayer. The 5′ end of the genomic RNA contains a methylated cap (m7GpppAmp), but it lacks a poly-A tail at the 3′ end [ 17 – 20 ]. The genomic RNA is ~11 kb in length and contains a single open reading frame (ORF) between two short non-coding regions (NCR) at 5′ and 3′ ends (Fig.…”

Section: Jev Structure and Genome Organisationmentioning

confidence: 99%

Molecular pathogenesis of Japanese encephalitis and possible therapeutic strategies

et al. 2022

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Japanese encephalitis virus (JEV), a single-stranded, enveloped RNA virus, is a health concern across Asian countries, associated with severe neurological disorders, especially in children. Primarily, pigs, bats, and birds are the natural hosts for JEV, but humans are infected incidentally. JEV requires a few host proteins for its entry and replication inside the mammalian host cell. The endoplasmic reticulum (ER) plays a significant role in JEV genome replication and assembly. During this process, the ER undergoes stress due to its remodelling and accumulation of viral particles and unfolded proteins, leading to an unfolded protein response (UPR). Here, we review the overall strategy used by JEV to infect the host cell and various cytopathic effects caused by JEV infection. We also highlight the role of JEV structural proteins (SPs) and non-structural proteins (NSPs) at various stages of the JEV life cycle that are involved in up- and downregulation of different host proteins and are potentially relevant for developing efficient therapeutic drugs. Graphical abstract

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Full-Length Genome Sequence of Japanese Encephalitis Virus Strain FC792, Isolated from Guangxi, China

Cited by 6 publications

References 6 publications

Phylogenetic molecular evolution and recombination analysis of complete genome of human parechovirus in Thailand

Phylogenetic molecular evolution and recombination analysis of complete genome of human parechovirus in Thailand

How Central Is the Domestic Pig in the Epidemiological Cycle of Japanese Encephalitis Virus? A Review of Scientific Evidence and Implications for Disease Control

Molecular pathogenesis of Japanese encephalitis and possible therapeutic strategies

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