Full off-axis red-green-blue digital holographic microscope with LED illumination

Dubois, Frank; Yourassowsky, Catherine

doi:10.1364/ol.37.002190

Cited by 63 publications

(33 citation statements)

References 17 publications

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“…To overcome the coherent noise, coherent lasers are replaced by light sources of partial coherence (partial temporal coherent light or partial spatial coherent light) [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. Nevertheless, a light emitting diode (LED) as a light source for DHMs has not yet been fully exploited [30][31][32][33][34][35][36][37]. The principle in LED light source (as well as with other short time coherence light source) illuminated DHM is optical path length compensation between an object and reference arms, which is achieved by using optics pairs in both arms, as in a Michelson or a Mach-Zehnder interferometer [32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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A reflection mode digital holographic microscope with light emitting diode (LED) illumination and off-axis interferometry is proposed. The setup is comprised of a Linnik interferometer and a grating-based 4f imaging unit. Both object and reference waves travel coaxially and are split into multiple diffraction orders in the Fourier plane by the grating. The zeroth and first orders are filtered by a polarizing array to select orthogonally polarized object waves and reference waves. Subsequently, the object and reference waves are combined again in the output plane of the 4f system, and then the hologram with uniform contrast over the entire field of view can be acquired with the aid of a polarizer. The one-shot nature in the off-axis configuration enables an interferometric recording time on a millisecond scale. The validity of the proposed setup is illustrated by imaging nanostructured substrates, and the experimental results demonstrate that the phase noise is reduced drastically by an order of 68% when compared to a He-Ne laser-based result.

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Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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“…12,31 Although in the demonstration we adopt a He-Ne laser as our light source to simplify the optical system, DAOLCI can be directly applied to diode laser or superluminescent diode by use of low-coherence DH. [32][33][34][35][36] Compared to the classical AO confocal imaging modality, DAOLCI does not require certain hardware pieces, such as a Shack-Hartmann wavefront sensor, deformable mirror, and He closed-loop feedbacks, and also opens the possibilities of adopting computational AO techniques [19][20][21] to correct for the aberration. As all the conventional AO ophthalmoscopes adopt, we have assumed the aberration happens at or close to the pupil.…”

Section: Discussionmentioning

confidence: 99%

Digital adaptive optics line-scanning confocal imaging system

Liu

Kim

2015

J. Biomed. Opt

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Abstract. A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack-Hartmann wavefront sensor and deformable mirror, and the closedloop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea.

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“…Also, coherent noise seems inevitable if laser is used as the light source. This can be addressed by use of low coherent light source [21,22,25]. …”

Section: Discussionmentioning

confidence: 99%

Phase aberration correction by correlation in digital holographic adaptive optics

2013

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We present a phase aberration correction method based on the correlation between the complex full-field and guide-star holograms in the context of digital holographic adaptive optics (DHAO). Removal of a global quadratic phase term before the correlation operation plays an important role in the correction. Correlation operation can remove the phase aberration at the entrance pupil plane and automatically refocus the corrected optical field. Except for the assumption that most aberrations lie at or close to the entrance pupil, the presented method does not impose any other constraints on the optical systems. Thus, it greatly enhances the flexibility of the optical design for DHAO systems in vision science and microscopy. Theoretical studies show that the previously proposed Fourier transform DHAO (FTDHAO) is just a special case of this general correction method, where the global quadratic phase term and a defocus term disappear. Hence, this correction method realizes the generalization of FTDHAO into arbitrary DHAO systems. The effectiveness and robustness of this method are demonstrated by simulations and experiments.

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Full off-axis red-green-blue digital holographic microscope with LED illumination

Cited by 63 publications

References 17 publications

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Digital adaptive optics line-scanning confocal imaging system

Phase aberration correction by correlation in digital holographic adaptive optics

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