Functional analysis of peach ACC oxidase promoters in transgenic tomato and in ripening peach fruit

Rasori, Angela; Bertolasi, B; Furini, Antonella; Bonghi, Claudio; Tonutti, Pietro; Ramina, Angelo

doi:10.1016/s0168-9452(03)00211-5

Cited by 30 publications

(10 citation statements)

References 33 publications

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“…Promoters from potato, pepper, peach, strawberry, melon and apple showed pronounced activity in tomato, usually similar to the gene expression pattern detected in the source plant [25][26][27][43][44][45][46]. Our results add to the evidence supporting that tomato is a useful model system for heterologous promoter testing, especially when promoters with fruit expression specificity are being analyzed.…”

Section: Discussionsupporting

confidence: 71%

Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

et al. 2020

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Background: Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development. Results: In this study, we report five citrus fruit-specific/preferential promoters for genetic engineering. Additionally, we have characterized a novel fruit-preferential promoter from plum. Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. Stable transformation in Micro-Tom tomato demonstrated that the candidate promoter regions exhibit differing levels of expression and with varying degrees of fruit specificity. Conclusions: Among the five candidate citrus promoters characterized in this study, the CitSEP promoter showed a fruit-specific expression pattern, while the CitWAX and CitJuSac promoters exhibited high fruit-preferential expression with strong activity in the fruit, weak activity in floral tissues and low or undetectable activity in other tissues. The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. Use of these fruit specific/ preferential promoters for genetic engineering can help with precise expression of beneficial genes and help with accurate prediction of the activity of new genes in host fruit plants.

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Section: Discussionsupporting

confidence: 71%

Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

et al. 2020

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“…The discrepancy between the effects of NaCl on PpERS1 promoter activity in tomato plants and PpERS1 transcript accumulation in peach might be attributed to GUS, which is a chimeric gene drive by PpERS1 promoter in some transgenic plants. A similar result was obtained when the promoters of peach genes that encode ACC oxidases were characterized in transgenic tomato, which was selected as the heterologous host given that it bears fleshy fruits that resemble those of peach trees (Rasori et al 2003). A previous study showed that activity GUS histochemical staining at different developmental stages in T1 tomato plants expressing fusion of the full-length PpESR1 promoter to GUS.…”

Section: Activity Of the Ppers1 Promoter In Transgenic Tomato Plantssupporting

confidence: 56%

Characterization of the expression of the stress-responsive <i>PpERS1</i> gene from peach and analysis of its promoter using transgenic tomato

Wang

Liu

Gong

et al. 2016

Plant Biotechnology

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The PpERS1 gene, which encodes an ethylene receptor and responds to abiotic and biotic stresses, was cloned from peach (Prunus persica L. Batsch cv Okubao). The genomic DNA sequence of PpERS1 comprises seven exons which are separated by six introns, interestingly alternative splicing of the first intron produced three different PpERS1 transcripts. In addition, a 2.8-kb sequence including the promoter of PpERS1 was isolated and analyzed by placing expressing of the GUS reporter gene under its control. Several putative cis-elements were identified in the promoter of PpERS1, including two ethylene-responsive elements (EREs), five W boxes, and four putative binding sites for MYB-type transcription factors. Deletion analysis indicated the presence of an enhancer element in the PpERS1 promoter. Temporal and spatial expression analysis of the PpERS1 promoter using histochemical GUS staining showed GUS activity in all tissues examined throughout the development of transgenic tomato plants. Exposure to various stresses caused similar changes in expression patterns in peach and transgenic tomato plants. Overall, our results suggested that PpERS1 gene might play important roles in response to multiple stresses via signal transduction mediated by ethylene receptors. The characterization of the PpERS1 promoter contributes to our understanding of the transcriptional regulation of this ethylene receptor in peach.

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“…In peach there are genes such as stony hard (Goffreda, 1992) and slow ripening (Brecht and Kader, 1984) that control ethylene and the rate of maturation, as are found in tomato (Lycopersicon esculentum), a well-studied fruit ripening system. A promising research approach would be to use the existing information on other crops to understand and identify genes in peach that control ripening (Rasori et al, 2003).…”

Section: Need For Better Postharvest Traitsmentioning

confidence: 99%

Trends in Stone Fruit Cultivar Development

Byrne

2005

horttech

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Despite the hundreds of existing stone fruit (Prunus spp.) cultivars used for fresh market, there is a continuing need to develop new stone fruit cultivars as the requirements of the industry change. Over the last 20 years there has been a shift toward private breeding as the public sector decreases its support of these long-range programs. As a result there are fewer public breeding programs and many of those still operating protect their releases and partially fund their programs with royalty payments. Other trends that are shaping the development of new stone fruit cultivars are a need for smaller or more easily managed tree architecture, a trend toward the use of fewer agricultural chemicals, the expansion of production zones into the milder winter zones to allow year-round availability of stone fruit, a general diversification of fruit types being marketed, the increased awareness of the health benefits of fruit consumption, the need for better and more consistent quality, and given the global marketing of these fruit the increased need for enhanced postharvest qualities. The breeding programs of the world are responding to these trends and working toward developing the cultivars for the world markets of the future.

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Functional analysis of peach ACC oxidase promoters in transgenic tomato and in ripening peach fruit

Cited by 30 publications

References 33 publications

Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

Characterization of the expression of the stress-responsive <i>PpERS1</i> gene from peach and analysis of its promoter using transgenic tomato

Trends in Stone Fruit Cultivar Development

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