Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis

Cao, Zheng; Ma, Yuhui; Wang, Xun; Xie, Yuqun; Ali, Maria Kanwal; He, Jin

doi:10.3389/fmicb.2015.00908

Cited by 40 publications

(63 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, we have found that cdaA from S. mutans could convert both ATP and ADP to c‐di‐AMP and had a high tolerance to an alkaline environment. Our results were consistent with other reports for Mycobacterium tuberculosis DisA protein (Bai et al ., ; Manikandan et al ., ) and Bacillus thuringiensis CdaS protein (Zheng et al ., ). However, the mechanism of c‐di‐AMP synthesis in B. thuringiensis CdaS seems to be distinct from that of M. tuberculosis DisA, since they could not detect either pApA or pppApA in CdaS catalytic reactions (Zheng et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans

Cheng

Zheng

Zhou

et al. 2015

Environmental Microbiology

View full text Add to dashboard Cite

Cyclic diadenosine monophosphate (c-di-AMP) has been implicated in the control of many important bacterial activities. However, the function of this molecule in Streptococcus mutans, the primary aetiological agent of human dental caries, is unknown. In this study, we identified and characterized a diadenylate cyclase, named CdaA, in S. mutans. Furthermore, we showed that in-frame deletion of the cdaA gene in S. mutans causes decreased c-di-AMP levels, increased sensitivity to hydrogen peroxide and increased production of extracellular polysaccharides. Global gene expression profiling revealed that more than 200 genes were significantly upregulated or downregulated (> 2.0-fold) in the cdaA mutant. Interestingly, genes with increased or decreased expression were clustered in cellular polysaccharide biosynthetic processes and oxidoreductase activity respectively. Notably, the expression of several genomic islands, such as GTF-B/C, TnSmu, CRISPR1-Cas and CRISPR2-Cas, was found to be altered in the cdaA mutant, indicating a possible link between these genomic islands and c-di-AMP signalling. Collectively, the results reported here show that CdaA is an important global modulator in S. mutans and is required for optimal growth and environmental adaption. This report also paves the way to unveil further the roles of c-di-AMP signalling networks in the biology and pathogenicity of S. mutans.

show abstract

Section: Discussionmentioning

confidence: 97%

Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans

Cheng

Zheng

Zhou

et al. 2015

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…B). To investigate whether the transcription of the prp operon is controlled by sporulation‐specific sigma factors, we assessed the transcriptional activity of the prp operon in the background of Δ sigH , Δ sigF , Δ sigE (Zheng et al ., ), and the two newly constructed mutants Δ sigG and Δ sigK . In this qualitative assay, we found that the prp operon was still actively transcribed in all five mutants (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

“…BMB171 and its mutants containing the transcriptional fusion plasmid pHT1K-P prp -lacZ were grown at 28 C in an orbital shaker at 200 rpm in 200 ml GYS medium with 25 μg ml −1 erythromycin. Four millilitres of cultures were sampled at 2 h intervals and used for the determination of β-galactosidase activity as described previously (Wang et al, 2013c;Zheng et al, 2015;Tang et al, 2016;Wang et al, 2019).…”

Section: Determination Of β-Galactosidase Activitymentioning

confidence: 99%

See 1 more Smart Citation

2‐Methylcitrate cycle: a well‐regulated controller of Bacillus sporulation

Cao

Du³

et al. 2019

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Bacillus thuringiensis is the most widely used eco‐friendly biopesticide, containing two primary determinants of biocontrol, endospore and insecticidal crystal proteins (ICPs). The 2‐methylcitrate cycle is a widespread carbon metabolic pathway playing a crucial role in channelling propionyl‐CoA, but with poorly understood metabolic regulatory mechanisms. Here, we dissect the transcriptional regulation of the 2‐methylcitrate cycle operon prpCDB and report its unprecedented role in controlling the sporulation process of B. thuringiensis. We found that the transcriptional activity of the prp operon encoding the three critical enzymes PrpC, PrpD, and PrpB in the 2‐methylcitrate cycle was negatively regulated by the two global transcription factors CcpA and AbrB, while positively regulated by the LysR family regulator CcpC, which jointly account for the fact that the 2‐methylcitrate cycle is specifically and highly active in the stationary phase of growth. We also found that the prpD mutant accumulated 2‐methylcitrate, the intermediate metabolite of the 2‐methylcitrate cycle, which delayed and inhibited sporulation at the early stage. Thus, our results not only revealed sophisticated transcriptional regulatory mechanisms for the metabolic 2‐methylcitrate cycle but also identified 2‐methylcitrate as a novel regulator of sporulation in B. thuringiensis.

show abstract

“…The method used for gene deletion in B. thuringiensis BMB171 was described previously (Zhang et al 2015;Zheng et al 2015). We take the deletion of yvcPQ for an example to briefly describe the procedure (Fig.…”

Section: Homing Endonucleases I-scei Mediated Markerless Gene Deletiomentioning

confidence: 99%

The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis

Zhang

Wang

et al. 2016

Arch Microbiol

Self Cite

View full text Add to dashboard Cite

YvcPQ is one of the two-component signal transduction systems that respond to specific stimuli and enable cells to adjust multiple cellular functions. It consists of a histidine kinase YvcQ and a response regulator YvcP. In this study, through searching the consensus sequence recognized by YvcP, we found four YvcP-binding motifs in the promoter regions of genes yvcR (BMB171_C4100), BMB171_C4385, kapD (BMB171_C4525) and BMB171_C4835 in Bacillus thuringiensis BMB171 which is a representative of Bacillus cereus group, and confirmed that these genes are regulated by YvcP. We compared the sequence of yvcPQ and its downstream genes in genus Bacillus, and found two different kinds of yvc locus, one was the yvcPQ-RS in B. subtilis species and the other was the yvcPQ-R-S1S2 in B. cereus group. Furthermore, we found that YvcP activates the transcription of yvcS1S2 (downstream of yvcR) to promote bacterial resistance to bacitracin and deletion of either yvcPQ operon or yvcS1S2 operon renders the bacterial cells more sensitive to bacitracin. This study enriched our understanding of both the YvcPQ's function and the mechanism of bacterial resistance to bacitracin.

show abstract

Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis

Cited by 40 publications

References 65 publications

Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans

Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans

2‐Methylcitrate cycle: a well‐regulated controller of Bacillus sporulation

The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis

Contact Info

Product

Resources

About