Functional characterization of<i>Ralstonia insidiosa</i>, a bona fide resident at the maternal-fetal interface

Parnell, Lindsay A.; Willsey, Graham G.; Joshi, Chetanchandra S.; Yin, Yin; Wargo, Matthew J.; Mysorekar, Indira U.

doi:10.1101/721977

Cited by 8 publications

(7 citation statements)

References 75 publications

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“…At the genus level, Pseudomonas , Bradyrhizobium , Microbacterium , Burkholderia and Ralstonia were the dominant genera of fetal gut, of which Ralstonia and Burkholderia were detected in the environmental control groups in recent studies ( 26 ). However, Ralstonia insidiosa , as a member of Ralstonia , was a resident at the maternal-fetal interface in another research ( 45 ). Indeed, we also detected a high abundance of Ralstonia pickettii and Ralstonia insidiosa in fetal gut.…”

Section: Discussionmentioning

confidence: 98%

Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation

Liu

He²,

Lan³

et al. 2023

Front. Immunol.

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Studies have shown that fetal immune cell activation may result from potential exposure to microbes, although the presence of microbes in fetus has been a controversial topic. Here, we combined metagenomic and virome techniques to investigate the presence of bacteria and viruses in fetal tissues (small intestine, cecum, and rectum). We found that the fetal gut is not a sterile environment and has a low abundance but metabolically rich microbiome. Specifically, Proteobacteria and Actinobacteria were the dominant bacteria phyla of fetal gut. In total, 700 species viruses were detected, and Human betaherpesvirus 5 was the most abundant eukaryotic viruses. Especially, we first identified Methanobrevibacter smithii in fetal gut. Through the comparison with adults’ gut microbiota we found that Firmicutes and Bacteroidetes gradually became the main force of gut microbiota during the process of growth and development. Interestingly, 6 antibiotic resistance genes were shared by the fetus and adults. Our results indicate the presence of microbes in the fetal gut and demonstrate the diversity of bacteria, archaea and viruses, which provide support for the studies related to early fetal immunity. This study further explores the specific composition of viruses in the fetal gut and the similarities between fetal and adults’ gut microbiota, which is valuable for understanding human fetal immunity development during gestation.

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Section: Discussionmentioning

confidence: 98%

Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation

Liu

He²,

Lan³

et al. 2023

Front. Immunol.

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“…Ralstonia has been reported to be part of the central microbiota of human breast milk and contributes to the development and maturation of the immune system ( 81 , 82 ). In humans, Ralstonia has been identified as a resident of the maternal-embryonic interface, such as in the endometrial decidua, placenta, chorion, and amnion ( 83 , 84 ).…”

Section: Discussionmentioning

confidence: 99%

Maternal transmission of bacterial microbiota during embryonic development in a viviparous lizard

Montoya-Ciriaco,

Hereira-Pacheco,

Estrada-Torres

et al. 2023

Microbiol Spectr

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The maternal transmission of microbiota during embryonic development of vertebrates is still poorly understood. Here, we used high-throughput sequencing of the 16S rRNA bacterial genes to determine the bacterial communities in the gastrointestinal tract and amniotic environment, i.e., the amniotic fluid, amniotic membrane and extraembryonic yolk, of embryos at the last stage of development of the viviparous lizard Sceloporus grammicus Wiegmann, 1828. We compared these communities to those found in the maternal intestine, mouth, cloaca, and the aseptic ventral skin as a control of the aseptic technique. Our results showed that bacterial 16S rRNA genes were present in the embryos of S. grammicus . Their diversity was lower and more similar in composition between individuals than those found in the maternal tissues. This suggests that a strong control exists on the transmission of bacteria from the mother to the embryos. We found 78% of the embryonic amplicon sequence variants (ASVs) in the maternal bacterial microbiota, suggesting that the transmission of bacteria from the mother to the embryos is a continuous process and some bacteria may have been transferred during early embryonic stages. The embryonic bacteria were found to overlap mostly with those found in the mouth and aseptic ventral skin of the mother, although it is difficult to conclude that the shared ASVs originated from these maternal tissues. Our study provides evidence of microbiota vertical transfer during embryonic development in the animal kingdom. It also highlights that this maternal transmission could be included in the maternal effects that impact the offspring. IMPORTANCE We investigated the presence and diversity of bacteria in the embryos of the viviparous lizard Sceloporus grammicus and their amniotic environment. We compared this diversity to that found in the maternal intestine, mouth, and cloaca. We detected bacterial DNA in the embryos, albeit with a lower bacterial species diversity than found in maternal tissues. Most of the bacterial species detected in the embryos were also found in the mother, although not all of them. Interestingly, we detected a high similarity in the composition of bacterial species among embryos from different mothers. These findings suggest that there may be a mechanism controlling the transmission of bacteria from the mother to the embryo. Our results highlight the possibility that the interaction between maternal bacteria and the embryo may affect the development of the lizards.

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“…R. pickettii has previously been identified in Australian amniotic fluid samples taken at caesarean section [ 9 ], while other species of Ralstonia sp. have been described as bona fide residents of the basal plate of the placenta [ 44 ]. A. wautersii has previously been isolated from human blood [ 45 ], while other species of this genus have been isolated from mid-trimester amniotic fluid [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Mid-Gestation Amniotic Fluid Cytokine and Bacterial DNA Profiles in Relation to Pregnancy Outcome in a Small Australian Cohort

Stinson

Berman

et al. 2023

Microorganisms

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A well-established association exists between intrauterine bacteria and preterm birth. This study aimed to explore this further through documenting bacterial and cytokine profiles in Australian mid-gestation amniotic fluid samples from preterm and term births. Samples were collected during amniocenteses. DNA was extracted and the full-length 16S rRNA gene was amplified and sequenced. Levels of the cytokines IL-1β, IL-6, IL-10, TNF-α and MCP-1 were determined using the Milliplex MAGPIX system. Bacterial DNA profiles were low in diversity and richness, with no significant differences observed between term and preterm samples. No differences in the relative abundance of individual OTUs between samples were identified. IL-1β and TNF-α levels were significantly higher in samples containing reads mapping to Sphingomonas sp.; however, this result should be interpreted with caution as similar reads were also identified in extraction controls. IL-6 levels were significantly increased in samples with reads that mapped to Pelomonas sp., whilst TNF-α levels were elevated in fluid samples from pregnancies that subsequently delivered preterm. Bacterial DNA unlikely to have originated from extraction controls was identified in 20/31 (64.5%) mid-gestation amniotic fluid samples. Bacterial DNA profiles, however, were not predictive of preterm birth, and although cytokine levels were elevated in the presence of certain genera, the biological relevance of this remains unknown.

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Functional characterization ofRalstonia insidiosa, a bona fide resident at the maternal-fetal interface

Cited by 8 publications

References 75 publications

Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation

Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation

Maternal transmission of bacterial microbiota during embryonic development in a viviparous lizard

Characterisation of Mid-Gestation Amniotic Fluid Cytokine and Bacterial DNA Profiles in Relation to Pregnancy Outcome in a Small Australian Cohort

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