Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Abdellatif, Islam M. Y.; Yuan, Shaoze; Renhu, Na; Yoshihara, Shizue; Hamada, Hideaki; Suzaki, Takuya; Ezura, Hiroshi; Miura, Kenji

doi:10.3390/ijms23031681

Cited by 14 publications

(12 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have shown that PHYs also respond to other abiotic stresses. Mutations in tomato PHYA and a double mutation in PHYB1B2 increased tolerance toward heat stress by inhibiting electrolyte leakage (EL) and enhancing several stress-responsive genes [ 18 ]. Moreover, a mutation in PHYB decreases the EL index and malondialdehyde (MDA) concentration and enhances cold tolerance in rice plants [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Tomato Drought Tolerance by PHYTOCHROME A and B1B2 Mutations

Abdellatif

Yuan

Yoshihara

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Drought stress is a severe environmental issue that threatens agriculture at a large scale. PHYTOCHROMES (PHYs) are important photoreceptors in plants that control plant growth and development and are involved in plant stress response. The aim of this study was to identify the role of PHYs in the tomato cv. ‘Moneymaker’ under drought conditions. The tomato genome contains five PHYs, among which mutant lines in tomato PHYA and PHYB (B1 and B2) were used. Compared to the WT, phyA and phyB1B2 mutants exhibited drought tolerance and showed inhibition of electrolyte leakage and malondialdehyde accumulation, indicating decreased membrane damage in the leaves. Both phy mutants also inhibited oxidative damage by enhancing the expression of reactive oxygen species (ROS) scavenger genes, inhibiting hydrogen peroxide (H2O2) accumulation, and enhancing the percentage of antioxidant activities via DPPH test. Moreover, expression levels of several aquaporins were significantly higher in phyA and phyB1B2, and the relative water content (RWC) in leaves was higher than the RWC in the WT under drought stress, suggesting the enhancement of hydration status in the phy mutants. Therefore, inhibition of oxidative damage in phyA and phyB1B2 mutants may mitigate the harmful effects of drought by preventing membrane damage and conserving the plant hydrostatus.

show abstract

Section: Introductionmentioning

confidence: 99%

Stimulation of Tomato Drought Tolerance by PHYTOCHROME A and B1B2 Mutations

Abdellatif

Yuan

Yoshihara

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Stomata facilitate gas and water exchange between plants and the surrounding environment [ 44 ], and lower stomatal conductance was often linked with a lower net photosynthetic rate [ 45 ]. CO 2 used for photosynthesis was acquired through the stomata opening [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, both iaa9-3 and iaa9-5 mutants have higher chlorophyll fluorescence under normal and heat stress conditions ( Figure 6 ). Higher chlorophyll fluorescence of both iaa9-3 and iaa9-5 can be attributed to lower MDA content ( Figure 5 ), which indicates less leaf oxidative damages [ 44 ], higher leaf chlorophyll and carotenoid contents, which helps prevent photo-oxidative stress [ 55 ], and higher stomatal conductance which prevents Fq/Fm reduction [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

SlIAA9 Mutation Maintains Photosynthetic Capabilities under Heat-Stress Conditions

Rahmat

Octavianis

Budiarto

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Tomato is one of the most widely consumed horticultural products. However, tomato is very sensitive to changes in temperature. Daily average temperatures above 32 °C severely reduced tomato plant growth, development, and productivity. Therefore, climate change-induced global warming is a major threat to future tomato production. Good photosynthetic capability under heat stress conditions is known to be a major sign of heat tolerance. Tomato INDOLE-ACETIC-ACID (SlIAA9) is a transcriptional repressor in auxin signaling. SlIAA9 mutation caused heightened endogenous auxin response and biosynthesis within plant tissues. In this study, we studied the photosynthetic capability of iaa9-3 and iaa9-5 mutants under heat-stress conditions. We discovered that both iaa9-3 and iaa9-5 could maintain their photosynthetic capability after 14 days of heat treatment (>40 °C), differing from Wild Type-Micro-Tom (WT-MT) tomato. Both iaa9 mutants had higher net photosynthetic rate, stomatal conductance, leaf total chlorophyll, leaf carotenoids, Fv/Fm value, and lower leaf MDA than WT-MT. These results suggested that the SlIAA9 mutation benefits plant adaptation to heat stress.

show abstract

“…One of the targets could be PHTOCHROME (PHY). PHYB has been identified as a thermosensor [ 84 , 85 ], and the phy mutant exhibited high tolerance to heat stress in Arabidopsis [ 86 ] and tomato [ 87 ]. This mutant information is important for the definition of targets of genome editing.…”

Section: Abiotic Stress Responses Revealed By Genome Editingmentioning

confidence: 99%

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Fuhrmann-Aoyagi

et al. 2022

CIMB

Self Cite

View full text Add to dashboard Cite

Global warming and climate change have severely affected plant growth and food production. Therefore, minimizing these effects is required for sustainable crop yields. Understanding the molecular mechanisms in response to abiotic stresses and improving agricultural traits to make crops tolerant to abiotic stresses have been going on unceasingly. To generate desirable varieties of crops, traditional and molecular breeding techniques have been tried, but both approaches are time-consuming. Clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALENs) are genome-editing technologies that have recently attracted the attention of plant breeders for genetic modification. These technologies are powerful tools in the basic and applied sciences for understanding gene function, as well as in the field of crop breeding. In this review, we focus on the application of genome-editing systems in plants to understand gene function in response to abiotic stresses and to improve tolerance to abiotic stresses, such as temperature, drought, and salinity stresses.

show abstract

Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Cited by 14 publications

References 97 publications

Stimulation of Tomato Drought Tolerance by PHYTOCHROME A and B1B2 Mutations

Stimulation of Tomato Drought Tolerance by PHYTOCHROME A and B1B2 Mutations

SlIAA9 Mutation Maintains Photosynthetic Capabilities under Heat-Stress Conditions

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Contact Info

Product

Resources

About