Functional dual hydrophilic dendrimer‐modified metal‐organic framework for the selective enrichment of N‐glycopeptides

Abstract

Analysis of protein glycosylation remains a significant challenge due to the low abundance of glycoproteins or N-glycopeptides. Here we have synthesized an amino-functionalized metal-organic framework (MOF) MIL-101(Cr)-NH whose surface is grafted with a hydrophilic dendrimer poly(amidoamine) (PAMAM) for N-glycopeptide enrichment based on the hydrophilic interactions. The selected substrate MOF MIL-101(Cr) owns high surface area which provides nice support for peptide adsorption. In addition, the MOF displayed a good hydrophilic property after being modified with amino groups. Most importantly, the grafted hydrophilic dendrimer PAMAM was firstly applied in the post-synthetic modification (PSM) of MOFs. And this functionalization route using macromolecular dendrimer opens a new perspective in MOFs design. Owing to its long dendritic chains and abundant amino groups, our material displayed dual hydrophilic property. In the enrichment of standard glycoprotein horseradish peroxidase (HRP) digestion, the functional MOF material was shown to have low detection limit (1 fmol/μL) and good selectivity when the concentration of nonglycopeptides was 100 fold higher than the target N-glycopeptides. All the results proved that MIL-101(Cr)-NH @PAMAM has great potential in the glycoproteome analysis. This article is protected by copyright. All rights reserved.

PPI-Dendrimer-Functionalized Magnetic Metal–Organic Framework (Fe3O4@MOF@PPI) with High Adsorption Capacity for Sustainable Wastewater Treatment

“…Dendrimers, as a class of regularly branched polymers, possess a well-defined size, a compact shape, and a large number of functional end groups. Dendrimers have also been used for wastewater treatment , and various environmental applications. , The polypropylene nonwoven functionalized with PPI dendrimers showed a great ability to remove textile dyes from the aqueous media …”

“…Dendrimers have also been used for wastewater treatment 43,44 and various environmental applications. 45,46 The polypropylene nonwoven functionalized with PPI dendrimers showed a great ability to remove textile dyes from the aqueous media. 22 In this study, we have hypothesized that the magnetic properties of Fe 3 O 4 nanoparticles along with a highly stable nanoporous structure of Zr-based MOF (UiO-66) possessing a high specific surface area could be combined to provide a highly efficient nanoporous magnetic adsorbent for wastewater treatment.…”

Poly(amidoamine) dendrimer‐coated magnetic nanoparticles for the fast purification and selective enrichment of glycopeptides and glycans

“…Thus, the alternative methods are necessary. In this regard, previous studies showed that magnetic‐based sorbents including dendrimers have decreased the time required for the sample preparation considerably since they can be easily separated from the mediums applying a magnetic field.…”

A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides

“…As shown in Table S3 (ESI†), the capture performance of the probe is in the top-rank and significantly superior to previously reported composites. 14,17,18,26–32…”

“…As shown in Table S3 (ESI †), the capture performance of the probe is in the top-rank and significantly superior to previously reported composites. 14,17,18,[26][27][28][29][30][31][32] N-Glycoproteomic profiling of clinical samples Hepatocellular carcinoma (HCC) is one of the most malignant cancers with high morbidity and mortality. The high death rate of HCC is primarily related to the fact that most HCC cannot be diagnosed promptly and accurately.…”