Functional Irreplaceability of Escherichia coli and Shewanella oneidensis OxyRs Is Critically Determined by Intrinsic Differences in Oligomerization

Sun, Weining; Fan, Yingying; Wan, Fen; Tao, Yizhi Jane; Gao, Haichun

doi:10.1128/mbio.03497-21

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…Potential reasons for this observation include that multiple heme-trafficking proteins contribute to delivering heme to the CCM apparatus, that heme is mobilized independent of any heme-trafficking proteins, and that the aforementioned mechanisms work in combination. In addition to the cyt c content, the activity of catalase KatB, which is a Type I hemoprotein dictating H 2 O 2 degradation in S. oneidensis ( 43 ), was also subjected to regulation of HtpA. Our findings are in line with the proposal that the assembly of catalase in vivo is assisted by chaperones ( 55 ), albeit such proteins had not been identified.…”

Section: Discussionsupporting

confidence: 83%

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Bacterial TANGO2 homologs are heme-trafficking proteins that facilitate biosynthesis of cytochromes c

Han

Guo

Wang

et al. 2023

mBio

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Heme, an essential molecule for virtually all living organisms, acts primarily as a cofactor in a large number of proteins. However, how heme is mobilized from the site of synthesis to the locations where hemoproteins are assembled remains largely unknown in cells, especially bacterial ones. In this study, with Shewanella oneidensis as the model, we identified HtpA (SO0126) as a heme-trafficking protein and homolog of TANGO2 proteins found in eukaryotes. We showed that HtpA homologs are widely distributed in all domains of living organisms and have undergone parallel evolution. In its absence, the cytochrome (cyt) c content and catalase activity decreased significantly. We further showed that both HtpA and representative TANGO2 proteins bind heme with 1:1 stoichiometry and a relatively low dissociation constant. Protein interaction analyses substantiated that HtpA directly interacts with the cytochrome c maturation system. Our findings shed light on cross-membrane transport of heme in bacteria and extend the understanding of TANGO2 proteins. IMPORTANCE The intracellular trafficking of heme, an essential cofactor for hemoproteins, remains underexplored even in eukaryotes, let alone bacteria. Here we developed a high-throughput method by which HtpA, a homolog of eukaryotic TANGO2 proteins, was identified to be a heme-binding protein that enhances cytochrome c biosynthesis and catalase activity in Shewanella oneidensis . HtpA interacts with the cytochrome c biosynthesis system directly, supporting that this protein, like TANGO2, functions in intracellular heme trafficking. HtpA homologs are widely distributed, but a large majority of them were found to be non-exchangeable, likely a result of parallel evolution. By substantiating the heme-trafficking nature of HtpA and its eukaryotic homologs, our findings provide general insight into the heme-trafficking process and highlight the functional conservation along evolution in all living organisms.

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Section: Discussionsupporting

confidence: 83%

“…We then asked if HtpA delivers heme to Type I hemoproteins. We tested this notion with catalase KatB, which contains heme b and is the predominant enzyme for scavenging hydrogen peroxide (H 2 O 2 ) in the cell ( 41 - 43 ). The H 2 O 2 consumption assay revealed that the Δ htpA strain decomposed H 2 O 2 at a rate slower than WT ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Bacterial TANGO2 homologs are heme-trafficking proteins that facilitate biosynthesis of cytochromes c

Han

Guo

Wang

et al. 2023

mBio

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“…LTTRs are typically homotetrameric proteins ( 7 , 9 ), but this oligomeric state is not universal and some members of the family are found in lower (e.g., dimer) and higher (e.g., octamer) oligomeric states ( 9 , 14 , 33 , 34 ). Ligand binding was also shown to modulate the oligomeric state of some LTTRs ( 7 , 33 ).…”

Section: Resultsmentioning

confidence: 99%

Emergence of an Auxin Sensing Domain in Plant-Associated Bacteria

Gavira

Rico-Jiménez

Ortega

et al. 2023

mBio

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“…To identify amino acid residues during complex formation, determine the types of interaction, and compile conformational maps, the software package "BIOVIA Discovery Studio" was used. The processes of visualization and clustering of obtained results were done with the help of FOREL algorithm-based program in Python environment due to the equation presented below:ΔGexp= -RT ln(1/K) (1)ΔG -Gibbs free energy, R -gas constant, Tabsolute temperature, K -binding constant[26].Statistical analyses:The carried-out experiments were done in 5 series with 3 repeats for each sample. For data analysis of all the TA new synthetic derivatives effects evaluation MS Excel tool was used.…”

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confidence: 99%

Tartaric acid new derivatives as prospective and safe alternative to antimicrobials for food products packing

Mikaelyan,

Babayan,

Grigoryan

et al. 2024

FFHD

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Introduction: Multi-drug resistance of pathogens and food spoilage bacteria is one of the most prevalent problems in contemporary science because the microbial adaptation potential is permanently being stimulated by the excessive antibiotic usage by mankind. In this research, seven new derivatives of tartaric acid (TA) were studied. Their high activity against the multi-drug resistant bacteria and the biodegradation potential by soil microflora was demonstrated.Objective: Based on multiple literature data about aldaric acids antimicrobial properties, for combating pan-drug and especially multi-drug resistant pathogenic microbes, natural L-tartaric acid was target-derivatized by cyclohexyl-, phenyl-, benzyl- and ethanolamine- functional groups into complex amino salts and cyclic imides, which have demonstrated the significant bactericidal and bacteriostatic activity against the spoilage agent bacteria, as well as phytopathogenic and human opportunistic pathogens.Results: In vitro analyses of TA ethanolamine- benzyl-, cyclohexyl- and phenyl- imides and complex amino salts have shown that the mentioned substances are highly active agents for combating multi-drug resistant Gram-positive and Gram-negative pathogens and food spoilage microbes, such as: Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Klebsiella pneumonia, Salmonella enteritidis, Staphylococcus aureus, etc. These compounds are biodegradable by P. chlororaphis group non-pathogenic bacteria and their resistance is ensured by genes of nucleoid as well as are not transmitted by plasmids. In silico analyses have demonstrated their alternative mechanism of action. Conclusions: The results suggest that TA new derivatives can be potentially recommended as safe alternative antimicrobials for food packaging.Keywords: tartaric acid imides, tartaric acid complex aminosalts, spoilage microorganisms, antimicrobial activity, multi-drug resistance.

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Functional Irreplaceability of Escherichia coli and Shewanella oneidensis OxyRs Is Critically Determined by Intrinsic Differences in Oligomerization

Abstract: Evolution may drive homologous proteins to be functionally nonexchangeable in different organisms. However, much is unknown about the mechanisms underlying this phenomenon beyond amino acid substitutions.

Cited by 6 publications

References 48 publications

Bacterial TANGO2 homologs are heme-trafficking proteins that facilitate biosynthesis of cytochromes c

Bacterial TANGO2 homologs are heme-trafficking proteins that facilitate biosynthesis of cytochromes c

Emergence of an Auxin Sensing Domain in Plant-Associated Bacteria

Tartaric acid new derivatives as prospective and safe alternative to antimicrobials for food products packing

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