Functional properties, structural studies and chemo-enzymatic synthesis of oligosaccharides

Zhao, Chao; Wu, Yijing; Liu, Xiaoyan; Liu, Bin; Cao, Hui; Yu, Hua; Sarker, Satyajit D.; Nahar, Lutfun; Xiao, Jianbo

doi:10.1016/j.tifs.2017.06.008

Cited by 93 publications

(53 citation statements)

References 127 publications

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“…The xynA enzyme, a member of the family GH10 of xylanases, in addition to its xylanolytic activity, displays activities against the xylan substrate previously thought to be specific to the affinity of binding to the cellulose substrate (Zhao et al 2017). It has been suggested that the derivative xynADCBM without CBM components can lead to the production of quality paper in the paper industry for pulp bleaching pretreatment.…”

Section: Resultsmentioning

confidence: 99%

Inter domain interactions influence the substrate affinity and hydrolysis product specificity of xylanase from Streptomyces chartreusis L1105

et al. 2020

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Purpose: This study investigated the influence of inter-domain interactions on the substrate affinity and hydrolysis product specificity of xylanase. Methods: Genes encoding a GH10 endo-xylanase from Streptomyces chartreusis L1105 xynA and its truncated derivative were cloned and expressed in Escherichia coli. The catalytic activities of the enzyme (xynA) and the derivative xynADCBM, lacking the carbohydrate binding module (CBM), were assessed to evaluate the role of CBM in xynA. Results: Recombinant xynA (44 kDa) was found to be optimally active on beechwood xylan at 65°C with pH 7.7, while xynADCBM (34 kDa) exhibited optimal activity at 65°C with pH 7.2. Additionally, xynA and xynADCBM were found to be highly thermostable at 40-60°C, each retaining 80% of their original activity after 30 min. The xynADCBM without the CBM domain was highly efficient at hydrolyzing xylan to produce xylobiose (over 67%), which may be because the CBM domain facilitates substrate binding with xylanase. Meanwhile, the xylan hydrolysis efficiency of xynADCBM was higher than that of xynA. Conclusion: These findings showed that the CBM domain with non-catalytic activity has no significant effect on the characteristics of the enzyme at optimum pH and pH tolerance. It has also been suggested that the derivative xynADCBM without CBM components can promote hydrolysis of xylan to yield xylooligosaccharides, which has great potential economic benefits.

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Section: Resultsmentioning

confidence: 99%

Inter domain interactions influence the substrate affinity and hydrolysis product specificity of xylanase from Streptomyces chartreusis L1105

et al. 2020

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“…Plants synthesise phytochemicals as part of their defence against pathogens, and many of these have effective antimicrobial activities. The biodiversity of the sources of phytochemicals provides unique and renewable resources for the discovery of novel biological activities (Bacanlı et al 2017;Chen et al 2018;Curti et al 2017;Vinayagam et al 2017;Zhao et al 2017). In this review we will focus on the antiadhesion and anti-biofilm activities of some of the known bioactive phytochemicals.…”

Section: Phytochemicals For the Prevention Of Biofilm Formationmentioning

confidence: 99%

Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces

et al. 2020

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Biofilms provide a protective environment for pathogens such as Campylobacter jejuni, the most prevalent foodborne pathogen, and biofilm formation can enhance bacterial survival in hostile environments. Adhesion of bacteria to the different materials of industrial surfaces is the first step in biofilm formation. Modulation of bacterial adhesion and biofilm formation thus represent important targets in alternative control strategies for reduction of pathogens in food-processing environments. With the high prevalence of C. jejuni and the lack of effective control measures, new control strategies are needed to block adhesion and biofilm formation on food contact surfaces in the food industry, with a focus here on natural antimicrobial phytochemicals. Plants remain a poorly recognized yet vast source of such antimicrobials. Valuable phytochemicals can be obtained directly from plant materials but also from agro-food by-products and waste materials. These materials represent a source of important plant bioactive phytochemicals that are effective for prevention of bacterial adhesion. In this review, we will focus on the anti-adhesion activities of phytochemicals targeted against C. jejuni, on the appropriate methodologies to determine anti-adhesion effects of phytochemicals, on the mechanisms of C. jejuni adhesion, and thus possible targets for reduction and control of this foodborne pathogen in food processing environments.

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“…The most common phytoconstituents are anthraquinones, carotenoids, catechins, flavonoids, ginsenosides, indoles, lignans, polyphenol, phenolic acids, phenylpropanoids, saponins etc. (Xiao, 2017;C. Zhao et al, 2017).…”

Section: Emre Of Escherichia Colimentioning

confidence: 99%

Mechanism and challenges associated with adaptation and evolution of drug-resistant bacteria: an overview

Kapil

Kumar

Sharma

2020

APJMBB

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Antimicrobial resistance is one of the leading challenges in the human healthcare segment. Advances in antimicrobial resistance studies have revealed various intrinsic, adaptive or acquired factors to be involved for pathogenicity. Antimicrobial agents are either bactericidal or bacteriostatic in action and prescribed according to the mode of action. Various factors are confined for the antimicrobial activity of these agents via biochemical, mechanical, physiological and molecular mechanisms. Microbial cell expresses a number of alternates responsible for the evolution of resistance against these agent activities involving cell surface modifications, enzyme inhibitions, modifications in efflux system, protein carriers and mutations in nucleic acids. Apart from this, the successful adaptations of such microbes have also been observed with the transfer of responsible genes through miscellaneous operations such as vertical evolution, horizontal gene transfer, co-selection, compensatory and random mutation. In addition, alterations or modifications in biochemical and physiological mechanisms at cellular levels are also responsible for antibiotic resistance. This article briefly shows the present scenario of antimicrobial resistance and the alternatives to overcome this global issue in future.

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Functional properties, structural studies and chemo-enzymatic synthesis of oligosaccharides

Cited by 93 publications

References 127 publications

Inter domain interactions influence the substrate affinity and hydrolysis product specificity of xylanase from Streptomyces chartreusis L1105

Inter domain interactions influence the substrate affinity and hydrolysis product specificity of xylanase from Streptomyces chartreusis L1105

Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces

Mechanism and challenges associated with adaptation and evolution of drug-resistant bacteria: an overview

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