Fundamental investigation of a novel drug delivery system, a transdermal delivery system with jet injection

Inoue, Naoko; Kobayashi, Daisuke; Kimura, Masayuki; Toyama, Machiji; Sugawara, Isamu; Itoyama, Shinji; Ogihara, Masahiko; Sugibayashi, Kenji; Morimoto, Yasunori

doi:10.1016/0378-5173(96)04488-2

Cited by 22 publications

(10 citation statements)

References 10 publications

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“…Furthermore, our study demonstrated that the interval time prior to the irradiation may be much shorter (1.5 h) with the needle-free injector than with the conventional method (3 h), which indicates that the onset of therapeutic effects following administration using the needle-free injector is much faster than using external application. The same conclusion was obtained by Inoue et al (26) who compared the needle-free injector with transdermal patches.…”

Section: Discussionsupporting

confidence: 83%

Needle-free injection of 5-aminolevulinic acid in photodynamic therapy for the treatment of condylomata acuminata

Wang

et al. 2013

Experimental and Therapeutic Medicine

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The external application of 5-aminolevulinic acid (ALA) in photodynamic therapy (PDT) results in a shallow penetration depth in thick or extensive condylomata acuminata (CA) lesions, thus demonstrating a poor therapeutic effect for those patients. To compare the efficacy of needle-free injection with external application of ALA in PDT for the treatment of CA, 160 CA patients with thick or extensive warts received ALA-PDT by means of external application or needle-free injection of ALA, respectively. The complete response (CR) rate and recurrence rate in the two groups were analyzed. The CR rate after the first treatment in the needle-free injection group (68.8%) was significantly higher compared with that in the external application group (52.5%; P=0.035). The recurrence rates in the needle-free injection group and external application group were 4.1 and 15.4%, respectively (P=0.022). The needle-free injection of ALA increases the therapeutic effect of PDT for CA patients with thick or extensive lesions. It shortens the treatment time and reduces the recurrence rate, and has great potential in the treatment of CA.

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Section: Discussionsupporting

confidence: 83%

Needle-free injection of 5-aminolevulinic acid in photodynamic therapy for the treatment of condylomata acuminata

Wang

et al. 2013

Experimental and Therapeutic Medicine

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“…Lipid-enhanced DNA delivery has so far not been successful, primarily because of relatively low efficiency and lipid and DNA toxicity at higher doses [85,86]. The particle gun propels micron-sized gold particles coated with DNA into target tissues [3]. Drawbacks of this technology include relatively low DNA-carrying capacity, and limited depth penetration of the particles (less than 1 mm in skin).…”

Section: Intracellular Dna Delivery For Gene Therapy and Dna Vaccinesmentioning

confidence: 99%

“…Therefore, other approaches have focused on improving cellular uptake of the drug, thus diminishing the requisite extracellular concentration to achieve a therapeutic effect. Strategies employed to that end include chemical modifications of the drug [1], improved drug formulations [2] and the use of drug delivery devices [3,4].…”

Section: Introductionmentioning

confidence: 99%

Nikolai Sergeevich Dikanskii (on his sixtieth birthday)

Balakin¹,

Барков²,

Dimov³

et al. 2001

Phys.-Usp.

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The effectiveness of potentially powerful therapeutics, including DNA, is often limited by their inability to permeate the cell membrane efficiently. Electroporation (EP) also referred to as 'electropermeabilization' of the outer cell membrane renders this barrier temporarily permeable by inducing 'pores' across the lipid bilayer. For in vivo EP, the drug or DNA is delivered into the interstitial space of the target tissue by conventional means, followed by local EP. EP pulses of micro-to millisecond duration and field strengths of 100-1500 V cm −1 generally enhance the delivery of certain chemotherapeutic drugs by three to four orders of magnitude and intracellular delivery of DNA several hundred-fold. We have used EP in clinical studies for human cancer therapy and in animals for gene therapy and DNA vaccination. Late stage squamous cell carcinomas of the head and neck were treated with intratumoural injection of bleomycin and subsequent EP. Of the 69 tumours treated, 25% disappeared completely and another 32% were reduced in volume by more than half. Residence time of bleomycin in electroporated tumours was significantly greater than in non-electroporated lesions. Histological findings and gene expression patterns after bleomycin-EP treatment indicated rapid apoptosis of the majority of tumour cells. In animals, we demonstrated the usefulness of EP for enhanced DNA delivery by achieving normalization of blood clotting times in haemophilic dogs, and by substantially increasing transgene expression in smooth muscle cells of arterial walls using a novel porous balloon EP catheter. Finally, we have found in animal experiments that the immune response to DNA vaccines can be dramatically enhanced and accelerated by EP and co-injection of micron-sized particles. We conclude that EP represents an effective, economical and safe approach to enhance the intracellular delivery, and thus potency, of important drugs and genes for therapeutic purposes. The safety and pharmaco-economic profile of EP compares favourably with other drug and DNA delivery methods.

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“…The injector¯ushes the drug solution into viable skin tissue through the skin barrier, the stratum corneum, using high pressure, so that the drug is rapidly absorbed into cutaneous blood vessels. Previously, we have applied such an injection system to the transdermal delivery of poorly absorbable drugs through the stratum corneum (Inoue et al 1996). Brie¯y, physiological saline without the drug was ®rst administered by the jet injector to make a pore or permeation pathway and then the drug solution was applied over the pretreated region of skin.…”

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confidence: 99%

Synergistic Effects of Iontophoresis and Jet Injector Pretreatment on the In-vitro Skin Permeation of Diclofenac and Angiotensin II

Sugibayashi

Kagino

Numajiri

et al. 2000

Journal of Pharmacy and Pharmacology

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A non-needle syringe (jet injector) was utilized to increase skin permeation of drugs by iontophoresis. Briefly, physiological saline was initially flushed by the injector to make a pore in the stratum corneum of excised hairless rat skin, and the iontophoretic skin permeation of two model compounds, sodium diclofenac and angiotensin II, was followed using a 2-chamber diffusion cell. Constant voltage and constant current iontophoresis treatments were evaluated. Pretreatment using the jet injector alone resulted in about 13- and 22-fold increases in the steady-state flux of diclofenac and angiotensin II, respectively, through the skin, compared with non-treated controls. Jet injector pretreatment with constant voltage iontophoresis further enhanced skin permeation of diclofenac and angiotensin II, and the enhancement was also greater than that by constant voltage iontophoresis alone. Thus, a synergistic effect was observed. The ratio of enhancement was greater compared with the control. Jet injector pretreatment with constant current iontophoresis, however, did not always yield higher skin permeation of the drugs than injector pretreatment alone, although the lag time was shortened. The difference in the enhancement between the constant voltage- and constant current iontophoresis can be explained by the electric current through the excised skin. Constant current iontophoresis after a short period of constant voltage iontophoresis with multiple jet injector pretreatments may be the best way to increase drug permeability while preventing severe skin damage.

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Fundamental investigation of a novel drug delivery system, a transdermal delivery system with jet injection

Cited by 22 publications

References 10 publications

Needle-free injection of 5-aminolevulinic acid in photodynamic therapy for the treatment of condylomata acuminata

Needle-free injection of 5-aminolevulinic acid in photodynamic therapy for the treatment of condylomata acuminata

Nikolai Sergeevich Dikanskii (on his sixtieth birthday)

Synergistic Effects of Iontophoresis and Jet Injector Pretreatment on the In-vitro Skin Permeation of Diclofenac and Angiotensin II

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