Fundamentals and Applications of Atmospheric Pressure Plasmas

Keidar, Michael; Weltmann, Klaus‐Dieter; Macheret, Sergey

doi:10.1063/5.0065750

Cited by 15 publications

(4 citation statements)

References 40 publications

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“…Along with well-established processing using cold plasmas established at low pressures [17], cold plasmas at atmospheric pressure have paved the way for the efficient processing of different materials. CAP provides an active chemical environment close to room temperature, enabling physical and chemical interaction with the surface of materials-such as etching, deposition and activation [18]. Due to generating a diversity of reactive oxygen and nitrogen species (RONS), such as radicals, ions, excited atoms and molecules (O, OH, H 2 O 2 , O 3 , N 2 *, O − 2 , etc.…”

Section: Introductionmentioning

confidence: 99%

Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment

Miletić,

Puač,

Škoro

et al. 2023

Applied Sciences

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In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.

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Section: Introductionmentioning

confidence: 99%

Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment

Miletić,

Puač,

Škoro

et al. 2023

Applied Sciences

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“…Plasma in atmospheric pressure is a key technology supporting many innovations in industrial applications because of its generation of electric fields, optical radiation, heat, and reactive chemical species 1 – 3 . Recently, the plasma systems have been explored for application in the fields of medicine and biology as an effective tool for wound healing 4 , 5 or cancer treatment 6 , 7 .…”

Section: Introductionmentioning

confidence: 99%

Potential generation of nano-sized mist by passing a solution through dielectric barrier discharge

Watanabe

Tanaka

Miyaji

et al. 2022

Sci Rep

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Plasma medicine, a therapeutic technology that uses atmospheric-pressure plasma, is attracting much attention as an innovative tool for the medical field. Most of the plasma biomedical tools use direct effects, such as heat, optical stimulation, and reactive chemical species, on the lesion. Nanoparticulation techniques using indirect action by plasma, i.e., generation of electric fields, have the potential to be applied to promote transdermal absorption, where drugs pass through the barrier function of skin and penetrate into internal tissues. Here, we show a method to directly generate the nano-sized mist by passing a solution through the dielectric barrier discharge. This method enables us to produce the mist potentially in the nanometer size range for both water-based and oil-based solutions. Ease of mist generation was influenced by the plasma-induced changes in physical and chemical characteristics, including electrical conductivity, viscosity, and chemical species. We anticipate the developed method for nano-sized mist generation to provide a technique in the applications of the transdermal absorption system, including those related to pharmaceuticals and cosmetics.

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“…In principle, this plasma provides an active chemical environment at near room temperature and at atmospheric pressure enabling physical and chemical interaction with the surface of materials -such as etching, deposition, activation etc. [20]. It is now well known that cold plasmas at atmospheric pressure generate a diversity of reactive oxygen (ROS) and nitrogen species (RNS) such as radicals, ions, excited atoms and molecules (O, OH, H 2 O 2 , O 3 , N 2 *, O 2 − etc.)…”

Section: Introductionmentioning

confidence: 99%

Assessment of the potential of cold plasma-pretreated beta-tricalcium phosphate in combination with periodontal ligament stem cells to enhance bone regeneration in vivo

Miletić

Puаč

Škoro

et al. 2022

Preprint

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Objectives A new strategy in the field of regenerative bone tissue medicine involves the combination of artificial bone substitutes and progenitor cells. In this approach the positive interaction of biomaterials and cells is crucial for successful bone regeneration. Materials and methods Herein, we examined if cold atmospheric plasma (CAP)-pretreated beta-tricalcium phosphate (β-TCP) alone, or in combination with periodontal ligament stem cells (PDLSCs), increases the bone regeneration in rabbit calvarial critical-size defect model. After two and four weeks of bone regeneration we analyzed and compared the bone formation in experimental groups using histology and histomorphometry. In addition, we used immunohistochemistry to analyze the protein expression of bone healing markers. Results After two and four weeks of bone regeneration, the results of histological and histomorphometric, analysis demonstrated significantly higher bone regeneration capacity and absence of inflammatory reaction in the implant of plasma-treated β-TCP in combination with PDLSCs compared to the β-TCP alone. Furthermore, immunohistochemical analysis showed the highest percentages of immunostained cells for the bone healing markers, bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin in the defects filled with CAP-treated β-TCP and PDLSC which additionally support beneficial therapeutic effects of such combined approach on osteoregeneration. Conclusion The CAP-pretreated β-TCP implant seeded with PDLSCs shows significantly higher bone regeneration capacity and absence of inflammatory reaction in vivo in comparison to the β-TCP alone. Clinical relevance Cold atmospheric plasma could be an effective method of treating β-TCP to improve the interaction of the material with stem cells enhancing regeneration of the bone.

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Fundamentals and Applications of Atmospheric Pressure Plasmas

Cited by 15 publications

References 40 publications

Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment

Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment

Potential generation of nano-sized mist by passing a solution through dielectric barrier discharge

Assessment of the potential of cold plasma-pretreated beta-tricalcium phosphate in combination with periodontal ligament stem cells to enhance bone regeneration in vivo

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