Fungal nitrilases as biocatalysts: Recent developments

Martı́nková, Ludmila; Vejvoda, Vojtěch; Kaplan, Ondřej; Kubáč, David; Malandra, Anna; Cantarella, Maria; Bezouška, Karel; Křen, Vladimı́r

doi:10.1016/j.biotechadv.2009.04.027

Cited by 55 publications

(30 citation statements)

References 73 publications

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“…While much information is available on the structure and function of bacterial nitrilases (Banerjee et al, 2002;Singh et al, 2006), a lesser amount of findings is available for nitrilases from filamentous fungi. Martínková et al (2009) reviewed the current knowledge of these enzymes by examining findings on enzyme screening, production, purification and immobilization and prospective applications in the field of biocatalysis. In particular, they investigated the potentiality of fungal nitrilase and compared their performance with some from bacterial origins.…”

Section: Enzymes As Decontaminating Agentsmentioning

confidence: 99%

Role of Enzymes in the Remediation of Polluted Environments

Rao

Scelza

Scotti

et al. 2010

J. Soil Sci. Plant Nutr.

177

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Environmental pollution is growing more and more due to the indiscriminate and frequently deliberate release of hazardous, harmful substances. Research efforts have been devoted to develop new, low-cost, low-technology, eco-friendly treatments capable of reducing and even eliminating pollution in the atmosphere, the hydrosphere and soil environments. Among biological agents, enzymes have a great potentiality to effectively transform and detoxify polluting substances because they have been recognized to be able to transform pollutants at a detectable rate and are potentially suitable to restore polluted environments. This brief review will examine some classes of pollutants and enzymes capable of transforming them effectively into innocuous products. Particular attention will be devoted to pollutants with a high polluting potential such as polyphenols, nitriles, PAHs, cyanides and heavy metals. The enzymatic processes developed and implemented in some of these detoxification treatments will be examined in details. The main advantages as well as the main drawbacks that are still present in the extensive application of enzymes in the in situ restoration of polluted environments will be discussed.

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Section: Enzymes As Decontaminating Agentsmentioning

confidence: 99%

Role of Enzymes in the Remediation of Polluted Environments

Rao

Scelza

Scotti

et al. 2010

J. Soil Sci. Plant Nutr.

177

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“…Selection on media with nitrile as the sole nitrogen source was a straightforward method for screening of nitrile-hydrolyzing microorganisms [26]. Thus, microbial enrichments in the present research were performed with glycinonitrile as the sole source of nitrogen (6 mM) and glucose (5 g/L) as a carbon source.…”

Section: Isolation Of Glycinonitrile-hydrolyzing Strainsmentioning

confidence: 99%

Isolation, Identification, and Culture Optimization of a Novel Glycinonitrile-Hydrolyzing Fungus—Fusarium oxysporum H3

Gong

Lü

Shi

et al. 2011

Appl Biochem Biotechnol

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Microbial transformation of glycinonitrile into glycine by nitrile hydrolase is of considerable interest to green chemistry. A novel fungus with high nitrile hydrolase was newly isolated from soil samples and identified as Fusarium oxysporum H3 through 18S ribosomal DNA, 28S ribosomal DNA, and the internal transcribed spacer sequence analysis, together with morphology characteristics. After primary optimization of culture conditions including pH, temperature, carbon/nitrogen sources, inducers, and metal ions, the enzyme activity was greatly increased from 326 to 4,313 U/L. The preferred carbon/nitrogen sources, inducer, and metal ions were glucose and yeast extract, caprolactam, and Cu(2+), Mn(2+), and Fe(2+), respectively. The maximum enzyme formation was obtained when F. oxysporum H3 was cultivated at 30 °C for 54 h with the initial pH of 7.2. There is scanty report about the optimization of nitrile hydrolase production from nitrile-converting fungus.

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“…Until a few years ago, all the fungal nitrilases reported were aromatic nitrilases [18]. It is only since 2011 that arylacetonitrilases have been found in filamentous fungi by genome mining and expression of the artificially synthesized genes [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Potential of Fungal Arylacetonitrilases in Mandelic Acid Synthesis

2015

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The application of arylacetonitrilases from filamentous fungi to the hydrolysis of high concentrations of (R,S)-mandelonitrile (100-500 mM) was demonstrated for the first time. Escherichia coli strains expressing the corresponding genes were used as whole-cell catalysts. Nitrilases from Aspergillus niger, Neurospora crassa, Nectria haematococca, and Arthroderma benhamiae (enzymes NitAn, NitNc, NitNh, and NitAb, respectively) exhibited different degrees of enantio- and chemoselectivity (amide formation). Their enantio- and chemoselectivity was increased by increasing pH (from 8 to 9-10) and adding 4-10% (v/v) toluene as the cosolvent. NitAn and NitNc were able to convert an up to 500 mM substrate in batch mode. NitAn formed a very low amount of the by-product, amide (<1% of the total product). This enzyme produced up to >70 g/L of (R)-mandelic acid (e.e. 94.5-95.6%) in batch or fed-batch mode. Its volumetric productivities were the highest in batch mode [571 ± 32 g/(L d)] and its catalyst productivities in fed-batch mode (39.9 ± 2.5 g/g of dcw). NitAb hydrolyzed both enantiomers of 100 mM (R,S)-mandelonitrile at pH 5.0 and is therefore promising for the enantioretentive transformation of (S)-mandelonitrile. Sequence analysis suggested that fungal arylacetonitrilases with similar properties (enantioselectivity, chemoselectivity) were clustered together.

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Fungal nitrilases as biocatalysts: Recent developments

Cited by 55 publications

References 73 publications

Role of Enzymes in the Remediation of Polluted Environments

Role of Enzymes in the Remediation of Polluted Environments

Isolation, Identification, and Culture Optimization of a Novel Glycinonitrile-Hydrolyzing Fungus—Fusarium oxysporum H3

Exploring the Potential of Fungal Arylacetonitrilases in Mandelic Acid Synthesis

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