Fungal Tannase: Recent Advances and Industrial Applications

Dhiman, Sunny; Mukherjee, Gunjan; Kumar, Amit; Mukherjee, P; Verekar, Shilpa A.; Deshmukh, S. K.

doi:10.1007/978-981-10-4768-8_16

Cited by 9 publications

(8 citation statements)

References 50 publications

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“…It helps in the hydrolysis of the ester bond (galloyl ester of alcohol moiety) and depside bond (galloyl ester of gallic acid) present in hydrolysable tannins thereby releasing simple phenolic molecules like gallic acid and some gallic acid esters along with glucose molecule. 2,3 It also hydrolyzes various substrates like epicatechin gallate, epigallocatechin gallate, and various gallic acid esters such as digallic acid, methyl gallate, ethyl gallate, n-propyl gallate, isoamyl gallate, to release gallic acid. 4 Tannase being one of the industrially important hydrolases, it finds an immense range of applications in various industries such as food, animal feed, cosmetics, pharmaceutical, chemical, and leather industries.…”

Section: Introductionmentioning

confidence: 99%

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Thangavelu¹,

Hugar²,

Belur³

2022

J. Pure Appl. Microbiol.

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With an aim to isolate a tannase positive organism, the microbial mat growing on the stored areca extract leachate surface was screened. Once the tannase positive organism was isolated, it was identified by ITS/18S rRNA gene sequencing. Further, the enzyme was purified and examined for its biochemical properties. A potent extracellular tannase-producing yeast was isolated and was identified as Geotrichum cucujoidarum. After the shake flask studies, the enzyme activity of 4.42 U/ml and specific activity of 29.86 U/mg were achieved in a medium with tannic acid as an inducer. Later, ethanol (70%) precipitation followed by purification through FPLC using SEC 650 column resulted in 166.37 U/mg specific activity and a recovery of 50.54%. The purified enzyme was a monomer with a molecular weight of 63 kDa. The optimum pH and the temperature of the enzyme were found to be 5.0 and 30°C, respectively. The Michaelis-Menten constant (Km) was found to be 2.9 mM, and the turn over number (kcat) and catalytic efficiency (kcat/km) of the purified tannase were 102 S-1 and 35.17 mM-1S-1 respectively. Temperature and pH stability profiles of the enzyme, influence of various metal ions, chelators and surfactants on enzyme activity and kinetic constants of enzyme shows that the tannase produced from Geotrichum cucujoidarum is unique and is a potential candidate for further studies.

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Section: Introductionmentioning

confidence: 99%

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Thangavelu¹,

Hugar²,

Belur³

2022

J. Pure Appl. Microbiol.

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“…The latter ones are an intermediate group that share a structure combination between hydrolysable tannins and condensed tannins. To form complex tannins, catechin or epicatechin units are linked via glycosidic linkage to a gallotannin or an ellagitannin unit, thus commonly referred to as catechin tannins, the form that is generally found in tea leaves and tropical shrub legumes [ 2 ]. Either condensed or complex tannins are considered microbial inhibitors as they resist microbial attacks and are toxic to a variety of microorganisms [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…[ 6 ], Aureobasidium melanogenum [ 7 ], Blastobotrys ( Arxula ) adeninivorans [ 8 ], Kluyveromyces marxianus [ 9 ], Sporidiobolus ruineniae [ 10 ], and Rhodosporidium diobovatum [ 11 ]. Tannases are widely used in various industrial applications including gallic acid production, instant tea production, beer and juice clarification, and effluent treatment [ 2 ]. Not only free tannases were industrially applied, but also immobilized enzymes were trialed to reduce some processes and operating costs associated with the production.…”

Section: Introductionmentioning

confidence: 99%

Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

et al. 2021

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Previously, nine tannin-tolerant and tannase-producing yeasts were isolated from Miang; all produced cell-associated tannase (CAT) during growth in tannin substrate. Among which, only CAT from Sporidiobolus ruineniae showed better stability than its purified form. Yet, it is of particular interest to directly characterize CATs from the latter yeasts. In this study, four CATs from yeasts, namely Cyberlindnera rhodanensis A22.3, Candida sp. A39.3, Debaryomyces hansenii A45.1, and Cy. rhodanensis A45.3 were characterized. The results indicate that all CATs were produced within the same production yield (11 mU/mL). Most CATs exhibited similar pH and temperature optima and stabilities, except for CAT from Cy. rhodanensis A22.3. This CAT was assigned as acid-stable tannase due to its unusual optimum pH of 2.0 with pH stability and half-life thermostability in the range of pH 2.0–4.0, and 70 °C, respectively. All CATs demonstrated high substrate specificity toward epigallocatechin gallate and epicatechin gallate, thus forming epigallocatechin and epicatechin, respectively. Moreover, they showed operational stability to repeated use for up to five cycles without loss of the initial activity. Therefore, CATs from these yeasts could be useful for the extraction and biotransformation of tea catechins and related applications.

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“…A enzima tanino acil hidrolase (TAH; EC 3.1.1.20), comumente denominada de tanase, catalisa a hidrólise de ésteres e ligações depsídicas de taninos complexos e hidrolisáveis, como o ácido tânico, liberando glicose e ácido gálico no caso dos galotaninos, e ácido elágico na hidrólise de elagitaninos (GONZÁLEZ et al, 2016;DHIMAN et al, 2017). Estas enzimas podem ser obtidas de fonte vegetal, animal e microbiana (bactérias, leveduras e fungos filamentosos).…”

Section: Introductionunclassified

“…Contudo, a maioria das tanases disponíveis comercialmente são produzidas por espécies de Aspergillus cultivadas em Fermentação Submersa (FSbm) (KHUMAR et al, 2018). Potencialmente aplicadas em diversos setores industriais, as tanases podem ser empregadas na indústria de bebidas como, por exemplo, no processamento de chás instantâneos, como agente clarificador de sucos de frutas e em bebidas geladas a base de café, sendo responsável pela remoção de compostos fenólicos (DHIMAN et al, 2017). A aplicação mais importante das tanases é na produção de ácido gálico, o qual apresenta atividade antioxidante, antimicrobiana, anti-inflamatória, antitumoral e antidiabética (CHOUBEY et al, 2018).…”

Section: Introductionunclassified

Enzimas Digestivas E Distúrbios Fisiopatológicos Associados

Xavier¹,

Araújo²,

Sousa³

2021

Ensino E Pesquisa Em Bioquímica

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.

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Fungal Tannase: Recent Advances and Industrial Applications

Cited by 9 publications

References 50 publications

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

Enzimas Digestivas E Distúrbios Fisiopatológicos Associados

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