Further Observations on the Effects of Tumours on the Perchloric Acid Soluble Proteins of the Rat

Burston, D.; Apsey, M. E.; Maclagan, N. F.

doi:10.1038/bjc.1965.25

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…In recent years several groups of workers, using a variety of species, have studied specific plasma glycoproteins or glycoprotein fractions which were believed to contribute to the increase in proteinbound carbohydrate of plasma resulting from inflammation. The glycoproteins studied are now normally referred to as the acute phase reactants, and include substances such as haptoglobin (2)(3)(4)(5)(6)(7)(8), fibrinogen (9-1 I), the seromucoid fraction (8, 1 1-1 31, a,-globulins (14-161, the a,-acid glycoprotein of , and a variety of a,-acid glycoprotein preparations (I I, [23][24][25][26]. The increased content of many of the acute phase reactants, present in the plasma of animals suffering from inflammation, has since been attributed to an increased rate of synthesis of the proteins in question (8)(9)(10)13).…”

Section: Intaoduetionmentioning

confidence: 99%

Studies on the effect of inflammation on rat serum proteins

Ashton¹,

Jamieson²,

Friesen³

1970

Can. J. Biochem.

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Studies on the effect of inflammation on rat serum proteins. Can. J. Biochem. 48, 841-850 (1 970).The effect of turpentine-induced inflammation on rat serum proteins has been studied at short time periods of exposure to an inflammatory agent (5-96 h). There was an increase in protein-bound hexose and hexosamine of serum reaching a maximum at 48 h after administration of turpentine. Eight fractions were prepared from serum by a combination of chromatography on DEAE-cellulose and preparative electrophoresis on strips of gelatinized cellulose acetate. Most of the increase in protein-bound carbohydrate (77-86%) found in serum from experimental animals was located in three fractions following the fractionation procedure; immunological studies revealed the presence of fibrinogen and haptoglobin in two of these fractions. There was no evidence for the presence of a new protein in serum at short times of exposure to an inflanmatory agent. Proteins present in the perchloric acid soluble and seromucoid fractions of serum were found in five fractions, only two of which contributed to the increase in protein-bound carbohydrate of serum found as a result of inflammation. Studies involving incorporation of labelled precursors of glycoprotein biosynthesis into fractions from serum indicated that the most likely explanation for the increase in protein-bound carbohydrate of serum, as a result of inflammation, was an increase in the rate of synthesis of certain serum glycoproteins.

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Section: Intaoduetionmentioning

confidence: 99%

Studies on the effect of inflammation on rat serum proteins

Ashton¹,

Jamieson²,

Friesen³

1970

Can. J. Biochem.

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Talc injury: Changes in glucosamine and leucine incorporation into liver and plasma proteins

Kaltenbach

Bolmey

1973

Experimental and Molecular Pathology

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Serum glycoprotein level at different stages of tumour growth.

El-Ghaffar¹,

Assad²

1967

Br J Cancer

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THE relation between malignancy and serum glycoproteins has been studied by many workers. Shetlar, Foster, Kelly, Shetlar and Everett (1949) found that in a series of 105 malignancies the serum glycoprotein was raised in 96% of cases. Seibert, Seibert Atno and Campbell (1947) obtained similar results. Israel, Webster, and Maher (1949) also found that the serum glycoproteins were elevated in malignancy as well as in other diseases such as chronic infections. rheumatic fever, lhyperthyroidism, hepatic disease and in pregnancy. Abd El-Ghaffar, Awny and Abd El-Meguid (1961. unpublished data) also worked on a large series of malignant cases and found that the serum glycoprotein level was raised in almost all cases, though the degree of rise varied in different types of malignancy.Shetlar, Erwin and Everett (1950) working on rats bearing the Walker 256 carcinoma reported that the total non-glucosamine polysaccharide level in the serum increased as the tumour increased in size. Macbeth and Bekesi (1964) worked on the seromucoid fraction in the serum of rats bearing Walker 256 carcinoma and by means of serial analyses of its protein, hexosamine, hexose, sialic acid and fucose content, reported that all seromucoid components show a p)rogressive and marked increase in the serum, and that the seromucoid produced in response to aggressive tumour growth was chemically different as regards its carbohydrate composition. Harshman and Bryant (1964) found that the chromatographic behaviour of serum mucoproteins in animals bearing Walker 256 carcinoma differed significantly from those of normal serum. Weimer, Quinn, Redlich-Moshin and Nishihara (1957) also worked on male rats implanted with Wtalker 256 carcinoma and by serial determinations found a highly significant increase in the seromucoid fraction in the serum of animals with well established tumours with coincident decline in other glycoproteins, total serum protein, haemoglobin concentrations and haematocrit. Burston, Apsey and Maclagan (1965) reported a high level of serum glycoprotein in tumour-bearing rats together with increase in weight of liver and in its perchloric acid soluble protein, which reached a maximum on the thirteenth day after implantation.In the present work serial estimations of serum glycoprotein levels were made in mice before, and at intervals after inoculation with sarcoma 180 and Ehrlich's ascites tumours, till the death of the animals from the tumours. MATERIALS AND METHODSAnimnals.-Ordinary Swiss mice were used, 8-12 weeks old. Inoculation.-Sarcoma 180 was kindly supplied by N.C.I., Bethesda, Maryland. U.S.A., and was maintained by weekly transfer. The 7-day-old tumour was aseptically removed, cut into small fragments, each about 1 mm. diameter; one

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Further Observations on the Effects of Tumours on the Perchloric Acid Soluble Proteins of the Rat

Cited by 3 publications

References 9 publications

Studies on the effect of inflammation on rat serum proteins

Studies on the effect of inflammation on rat serum proteins

Talc injury: Changes in glucosamine and leucine incorporation into liver and plasma proteins

Serum glycoprotein level at different stages of tumour growth.

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