Fusion-inhibiting monoclonal antibodies and their relevant antigens in relation to sexual process of Dictyostelium discoideum

Aiba, Keisuke; Urushihara, Hideko; Yanagisawa, Keiji

doi:10.1111/j.1432-0436.1992.tb00769.x

Cited by 10 publications

(2 citation statements)

References 21 publications

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“…Later work found different antigens on the surface of the different mating‐types under conditions that promote the initial cell fusion to form zygotes (Urushihara et al. 1988; Aiba et al. 1992).…”

Section: Sexual Genetics Of Dictyostelidsmentioning

confidence: 99%

Genetics of sex determination in the social amoebae

Bloomfield

2011

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The social amoebae possess a sexual cycle that involves transient mutlicellularity: first a zygote attracts surrounding haploid amoebae to form a walled aggregate around it, and then cannibalizes these peripheral cells, eventually forming a dormant single-celled macrocyst. Self-fertile homothallic isolates occur as well as breeding groups of self-infertile heterothallic cells, which commonly have more than two mating types. The mating-type locus of the widely studied model organism Dictyostelium discoideum, which has three mating types, has recently been identified. Two of the three mating types are determined by single putative regulatory genes bearing no mutual similarity, while the third is specified by homologues of both of these genes. This is the first sexdetermining locus of an Amoebozoan to be described and, since none of the key regulators show homology to known proteins, may be a first glimpse of a novel mode of regulation used in these organisms. The sexual cycle of dictyostelids has been relatively neglected, but continues to yield much interesting biology as well as having the potential to add to the genetic tools available for the study of these organisms.

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Section: Sexual Genetics Of Dictyostelidsmentioning

confidence: 99%

Genetics of sex determination in the social amoebae

Bloomfield

2011

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“…In addition, the epitopes for many of the fusion-blocking mAbs have been shown to be carbohydrates (1,17). Since carbohydrates are present in massive quantities on the cell surface of cellular slime molds, and binding of lectins or antibodies could interfere sterically with physiological molecular interactions.…”

Section: Involvement Of Carbohydratesmentioning

confidence: 99%

Sexual Development of Cellular Slime Molds

Urushihara¹

1992

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Macrocyst formation represents sexual development of cellular slime molds and begins with fusion between cells of compatible mating types. Homothallic and heterothallic strains as well as bisexual and asexual ones have been described. Macrocyst development requires certain environmental conditions such as darkness and excessive humidity. Sexual cell fusion has been analyzed at a molecular level in Dictyostelium discoideum, and several cell surface proteins related to it have been identified. Some of them are common to both mating types, while others are specific to one or other type. The involvement of cell‐surface carbohydrates has also been suggested, though direct evidence for this is still lacking. Macrocyst formation is regulated by diffusible, pheromone like substances. Genetic studies on sexual development are scarce, probably because no suitable mutants have been available. However, several asexual mutants, as well as antibody and nucleotide probes, have recently been obtained, so mechanisms of sexual cell fusion may be understood in the near future. Considering the unique phylogenical position of cellular slime molds, analysis of sexual development in these organisms should contribute to the understanding of the mechanism and evolution of sexual reproduction systems.

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Biochemical Characterization of the Phagocytic Giant Cell Receptor forDictyostelium discoideumAmoebae: Identification by Cell Blotting

Lewis

Jamieson

1997

Biochemical and Biophysical Research Communications

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Fusion-inhibiting monoclonal antibodies and their relevant antigens in relation to sexual process of Dictyostelium discoideum

Cited by 10 publications

References 21 publications

Genetics of sex determination in the social amoebae

Genetics of sex determination in the social amoebae

Sexual Development of Cellular Slime Molds

Biochemical Characterization of the Phagocytic Giant Cell Receptor forDictyostelium discoideumAmoebae: Identification by Cell Blotting

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