Fusion of Spheroplasts and Genetic Recombination ofZymomonas mobilis

Cloning and Characterization of a Pair of Genes That Stimulate the Production and Secretion ofZymomonas mobilisExtracellular Levansucrase and Invertase

“…Ii) Strain Z6C is a spontaneous mutant derived from strain Z6 and constitutively produces the extracellular E2 and E3 in large quantities. 8 ) NTG-induced Sucmutants Z6C-S I and Z6C-S2, derived from strain Z6C, are unable to grow on sucrose on account of a substantial deficiency in the production of E2 and E3. 8 .7 kb) were used.…”

“…8 ) NTG-induced Sucmutants Z6C-S I and Z6C-S2, derived from strain Z6C, are unable to grow on sucrose on account of a substantial deficiency in the production of E2 and E3. 8 .7 kb) were used. Plasmid pZA22 is a shuttle vector between E. coli and Z. mohilis described previously.IO) E. coli MV1184.…”

“…These Sucmutants produced less than 0.01 % E2 and E3 relative to those produced by Z6C. 8 ) Occurrence of these mutants suggested the presence of some control genes governing production of extracellular E2 and E3.…”

Transformation ofZymomonas mobiliswith Plasmid DNA

“…For the cultivation of Zymomonas strains, RM medium (2% glucose, 1% yeast extract and 0.2% KH z P0 4 , pH6.0)5) or S medium (RM medium containing 0.5% MgS0 4 ' 7H z O and 20% sucrose) was used. 6 ) For regeneration, R medium (RM medium containing 16% sorbitol) was used. 6 ) Zymomonas was cultivated statically at 30°C.…”

“…6 ) For regeneration, R medium (RM medium containing 16% sorbitol) was used. 6 ) Zymomonas was cultivated statically at 30°C.…”