Gadolinium induced apoptosis of human embryo liver L02 cell line by ROS-mediated AIF pathway

Effects of gadolinium‐based MRI contrast agents on liver tissue

“…In addition, Laville et al reported that gadolinium does not give rise to oxidative stress in fish cells . Recent mammal studies have reported that gadolinium can induce cell apoptosis by means of endoplasmic reticulum stress, mitochondrial pathways, and oxidative stress . Xia et al reported that exposure of cells to gadolinium led to a rapid increase in intracellular ROS, leading to a rise in cellular Ca 2+ levels and the production of endoplasmic reticulum stress creators .…”

“…In previous toxicity studies, gadodiamide and gadoteric acid have been administered intravenously (iv) at doses above 0.6 mml/kg for 20 days. 8,18,23 In our study, we administered gadodiamide and gadoteric acid iv at the therapeutic dose of 0.2 ml/kg (equivalent to 0.1 mml/kg) for 20 days. 8,23 No procedure was performed on the healthy control group (Group 1).…”

“…Caspase‐3 is regarded as an irreversible terminal event before cell death and is used as a marker of apoptosis . Liu et al reported that ROS induce apoptosis . In addition, caspase, and particularly caspase‐3 activation, plays a vital role in apoptosis and may be considered a terminal event before cell death …”

“…20,21 Liu et al reported that ROS induce apoptosis. 18,19 In addition, caspase, and particularly caspase-3 activation, plays a vital role in apoptosis and may be considered a terminal event before cell death. 20,21 In the light of the above, the purpose of this study was to investigate, using histopathological and biochemical methods, the effects on liver tissue of the presumed oxidative stress induced by gadodiamide and gadoteric acid.…”

Lipid regulation effects of Polygoni Multiflori Radix, its processed products and its major substances on steatosis human liver cell line L02

“…This cell line could maintain characteristics and ultrastructure of normal liver cells after continuous passage (Ye et al, 1980;Li et al, 2007). L02 cell line has been shown to express many specific liver cell functions and could be used in many fields such as activity (Liu et al, 2006;Jin et al, 2009;Liu and Zhang, 2009;Chen et al, 2010;Ye et al, 2011), toxicity (Wang et al, 2008;Yuan et al, 2009) and artificial liver research (Bao-san et al, 2008). The steatosis L02 cells are widely used in the lipid metabolism regulation researches (GomezLechon et al, 2007;Shen et al, 2008;Pan et al, 2010;Zhang et al, 2011), especially for traditional Chinese medicine and their active compounds, such as saponin (Zhang et al, 2011), ursolic acid (Shen et al, 2008) and curcumin (Pan et al, 2010).…”