Gaining Acceptance of Novel Plant Breeding Technologies

Anders, Sven; Cowling, Wallace; Pareek, Ashwani; Gupta, Kapuganti Jagadis; Singla‐Pareek, Sneh L.; Foyer, Christine H.

doi:10.1016/j.tplants.2021.03.004

Cited by 45 publications

(27 citation statements)

References 47 publications

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“…The novelty and value of genomic editing in applied breeding has largely recognized. Nevertheless, before such technology could be fully implemented and largely produce practical results, it will be important that a science-based regulatory frame be applied consistently worldwide [147][148][149][150][151].…”

Section: Discussionmentioning

confidence: 99%

Breeding for Nutritional and Organoleptic Quality in Vegetable Crops: The Case of Tomato and Cauliflower

Natalini¹,

Acciarri²,

Cardi

2021

Agriculture

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Due to novel and more demanding consumers’ requirements, breeding of vegetable crops confronts new challenges to improve the nutritional level and overall appearance of produce. Such objectives are not easy to achieve considering the complex genetic and physiological bases. Overtime, plant breeders relied on a number of technologies and methods to achieve ever changing targets. F1 hybrid seed production allowed the exploitation of heterosis and facilitated the combination of resistance and other useful genes in a uniform outperforming variety. Mutagenesis and tissue culture techniques permitted to induce novel variation, overcome crossing barriers, and speed up the achievement of true-breeding lines. Marker-assisted selection was one of the milestones in fastening selection, starting from the early ’90s in almost all seed companies. Only recently, however, are novel omics tools and genome editing being used as cutting-edge techniques to face old and new challenges in vegetable crops, with the potential to increase the qualitative value of crop cultivation and solve malnutrition in 10 billion people over the next 30 years. In this manuscript, the evolution of breeding approaches in vegetable crops for quality is reviewed, reporting case studies in tomato (Solanum lycopersicum L.) and cauliflower (Brassica oleracea var. botrytis L.) as model systems for fleshy fruit and floral edible parts, respectively.

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Section: Discussionmentioning

confidence: 99%

Breeding for Nutritional and Organoleptic Quality in Vegetable Crops: The Case of Tomato and Cauliflower

Natalini¹,

Acciarri²,

Cardi

2021

Agriculture

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“…Agrobacterium strains have been improved to achieve increased plant transformation frequency through the development of binary vectors (Zambryski et al, 1982;Hoekema et al, 1983;Bevan, 1984;Komari et al, 2006), the development of ternary helper plasmids and superbinary vectors (Ishida et al, 1996;Anand et al, 2018;Zhang et al, 2019), the upregulation of virulence (vir) gene expression (Ishida et al, 1996;Van Der Fits et al, 2000;Ye et al, 2011;Vaghchhipawala et al, 2018), and the removal of negative factors of T-DNA transfer (Nonaka et al, 2019). Genome editing has also helped improve Agrobacterium strains themselves.…”

Section: Improvement Of Agrobacterium Strainsmentioning

confidence: 99%

“…For example, clustered regularly interspaced short palindromic repeats (CRISPR)-mediated lossof-function mutations in recA have generated an EHA105 strain with improved performance for maize transformation (Rodrigues et al, 2021). RecA-deficient strains are typically used to avoid the recombination of additional virulence genes from ternary helper plasmids with homologous sequences from the Ti plasmid (Mookkan et al, 2017;Anand et al, 2018;Sardesai et al, 2018), which allows their concomitant use with ternary vectors harbouring additional virulence genes and morphogenic regulators to increase plant regeneration.…”

Section: Improvement Of Agrobacterium Strainsmentioning

confidence: 99%

“…SDN-1 and SDN-2 generate small-sized random (SDN-1) or template-directed (SDN-2) mutations at predefined genomic loci and have thus been considered mimics of those resulting from natural DNA variation ( Eckerstorfer et al, 2021 ). Thus, a handful of countries consider crops modified by SDN-1 and SDN-2 as conventional plants ( Anders et al, 2021 ). Argentina, Chile, the USA, Canada, Brazil, Colombia, and Paraguay have already approved normative resolutions on genome editing ( Eckerstorfer et al, 2019 ), whereas the European Union relies on the legislation of GM organisms to restrict the cultivation of genome-edited crops ( Turnbull et al, 2021 ).…”

Section: Development Of Genetically Modified and Edited Varietiesmentioning

confidence: 99%

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Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

et al. 2021

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Over the past decades, advances in plant biotechnology have allowed the development of genetically modified maize varieties that have significantly impacted agricultural management and improved the grain yield worldwide. To date, genetically modified varieties represent 30% of the world’s maize cultivated area and incorporate traits such as herbicide, insect and disease resistance, abiotic stress tolerance, high yield, and improved nutritional quality. Maize transformation, which is a prerequisite for genetically modified maize development, is no longer a major bottleneck. Protocols using morphogenic regulators have evolved significantly towards increasing transformation frequency and genotype independence. Emerging technologies using either stable or transient expression and tissue culture-independent methods, such as direct genome editing using RNA-guided endonuclease system as an in vivo desired-target mutator, simultaneous double haploid production and editing/haploid-inducer-mediated genome editing, and pollen transformation, are expected to lead significant progress in maize biotechnology. This review summarises the significant advances in maize transformation protocols, technologies, and applications and discusses the current status, including a pipeline for trait development and regulatory issues related to current and future genetically modified and genetically edited maize varieties.

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“…It is also feasible to produce plants devoid of any foreign DNA by methods collectively called next-generation or new breeding techniques (NBTs) (Holme et al, 2019). Some of the NBTs target genes with a Cas9-coding DNA vector who does not integrate into the plant genome, or even a microinjected Cas9 protein and gRNAs, whereas other NBTs are based on non-targeted random mutations (Holme et al, 2019;Anders et al, 2021). The concept of "gene target" proved determinant in the understanding of some domesticated organisms.…”

Section: Basic Lessons From Agriculturementioning

confidence: 99%

Grand Challenges in Microalgae Domestication

Maréchal

2021

Front. Plant Sci.

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Gaining Acceptance of Novel Plant Breeding Technologies

Abstract: Current agricultural practices are unsustainable. Moreover, an enhanced ability to adapt to changing climates, enabling expansion of cultivation and yield resilience, is required to maintain and increase crop productivity.

Cited by 45 publications

References 47 publications

Breeding for Nutritional and Organoleptic Quality in Vegetable Crops: The Case of Tomato and Cauliflower

Breeding for Nutritional and Organoleptic Quality in Vegetable Crops: The Case of Tomato and Cauliflower

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Grand Challenges in Microalgae Domestication

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