Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance

Lu, Hongfang; Lim, Wei Seng; Wang, Jun; Tang, Ziyang; Zhang, Peng‐Chi; Leong, Kam W.; Chia, Ser Mien; Yu, Hanry; Mao, Hai‐Quan

doi:10.1016/s0142-9612(03)00404-6

Cited by 82 publications

(44 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5), that albumin secretion can be roughly estimated at 310 mg/ 10 6 cells/day. Direct comparisons with other studies are generally difficult mainly owing to the differences in the concentrations of growth factors or hormones, but this value approximately seems to be several times higher than some other previous spheroid-based culture systems, such as 40 mg/10 6 cells/day (Lu et al, 2003) and 70 mg/10 6 cells/day (Seo et al, 2005). Besides such spheroid cultures, sandwich collagen culture is another best system for hepatocyte culture (Dunn et al, 1992).…”

Section: Discussionmentioning

confidence: 84%

Stable immobilization of rat hepatocytes as hemispheroids onto collagen‐conjugated poly‐dimethylsiloxane (PDMS) surfaces: Importance of direct oxygenation through PDMS for both formation and function

Nishikawa

Yamamoto

Kojima

et al. 2007

Biotech & Bioengineering

View full text Add to dashboard Cite

The highly oxygen-permeable material, poly-dimethylsiloxane (PDMS), has the potential to be applied to cell culture microdevices, but cell detachment from PDMS has been a major problem. In this study, we demonstrate that a combination of collagen covalently immobilized PDMS and an adequate oxygen supply enables the establishment of a stable, attached spheroid (hemispheroid) culture of rat hepatocytes. The bottom PDMS surfaces were first treated with oxygen plasma, then coupled with aminosilane followed by a photoreactive crosslinker, and they were finally reacted with a collagen solution. X-ray photoelectron spectroscopy (XPS) and contact angle measurements showed that the covalent immobilization of collagen on the surface occurred only where the crosslinker had been introduced. On the collagen-conjugated PDMS surface, rat hepatocytes organized themselves into hemispheroids and maintained the viability and a remarkably high albumin production at least for 2 weeks of culture. In contrast, hepatocytes on the other types of PDMS surfaces formed suspended spheroids that had low albumin production. In addition, we showed that blocking the oxygen supply through the bottom PDMS surface inhibited the formation of hemispheroids and the augmentation of hepatocellular function. These results show that appropriate surface modification of PDMS is a promising approach towards the development of liver tissue microdevices.

show abstract

Section: Discussionmentioning

confidence: 84%

Stable immobilization of rat hepatocytes as hemispheroids onto collagen‐conjugated poly‐dimethylsiloxane (PDMS) surfaces: Importance of direct oxygenation through PDMS for both formation and function

Nishikawa

Yamamoto

Kojima

et al. 2007

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Micro BCA protein assay reagent kit was obtained from Pierce Chemical (USA). Primary hepatocytes were harvested from Wistar rats with a weight of about 275 g by a twostep collagenase perfusion protocol as described previously [10]. The cell viability was checked with Trypan Blue exclusion method.…”

Section: Methodsmentioning

confidence: 99%

Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface

Tan¹,

Teo²,

Liao

et al. 2005

Biomaterials

Self Cite

View full text Add to dashboard Cite

“…Their result demonstrated that it is possible to establish a serum-free system that is competent in facilitating epithelial-mesenchymal interaction of salivary tissue and facilitated by PVDF, the submandibular gland recombinant was able to sprout new branches without serum (Yang et al, 2010). Lu et al (2003) designed a PVDF surface coated with galactose-tethered pluronic polymer and investigated the efficacy of attachment of rat hepatocytes on PVDF membrane. Their results showed the potential of galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture.…”

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

613

389

View full text Add to dashboard Cite

Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

show abstract

Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance

Cited by 82 publications

References 25 publications

Stable immobilization of rat hepatocytes as hemispheroids onto collagen‐conjugated poly‐dimethylsiloxane (PDMS) surfaces: Importance of direct oxygenation through PDMS for both formation and function

Stable immobilization of rat hepatocytes as hemispheroids onto collagen‐conjugated poly‐dimethylsiloxane (PDMS) surfaces: Importance of direct oxygenation through PDMS for both formation and function

Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Contact Info

Product

Resources

About