Galanin overexpressing transgenic mice

Crawley, Jacqueline N.; Mufson, Elliott J.; Hohmann, John G.; Teklemichael, Dawit N.; Steiner, Robert A.; Holmberg, Kristina; Xu, Zhi‐Qing David; Blakeman, Karin Hygge; Xu, Xiao‐Jun; Wiesenfeld‐Hallin, Zsuzsanna; Bartfai, Tamás; Hökfelt, Tomas

doi:10.1054/npep.2002.0891

Cited by 48 publications

(26 citation statements)

References 67 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, mRNA expression does not correlate with posttranscriptional protein regulation or protein localization to nerve terminals. Ultimately, the most prominent areas of GalR1, GalR2, and GalR3 immunoreactivity correlate with the detection of mRNA transcripts in all published reports of galanin receptor mRNA distribution (Burgevin et al, 1995;Wang et al, 1997b;Kolakowski et al, 1998;Smith et al, 1998;O'Donnell et al, 1999;Zachariou et al, 2000;Crawley et al, 2002;Mennicken et al, 2002;Hohmann et al, 2003;Shen et al, 2003).…”

Section: Discussionmentioning

confidence: 58%

Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain

Hawes

Picciotto

2004

J of Comparative Neurology

View full text Add to dashboard Cite

The distribution of immunoreactivity for the three identified neuropeptide galanin receptors, GalR1, GalR2, and GalR3, was determined in areas of the mouse brain involved in drug addiction, including the ventral tegmental area (VTA), substantia nigra (SN), nucleus accumbens (NA), and locus coeruleus (LC). All three galanin receptors are found in the VTA, SN, NA, and LC; however, GalR1 protein is most highly represented in the VTA, NA, and SN, suggesting that GalR1 may play a predominant role in galanin-mediated regulation of dopamine neurotransmission. GalR1 and GalR3 protein levels are high in the LC, suggesting that these isoforms may be important for galanin-mediated regulation of noradrenergic transmission during opiate withdrawal. Although the distribution of GalR1, GalR2, and GalR3 largely recapitulates the pattern of galanin binding throughout the brain, some discrepancies exist, suggesting that another galanin receptor(s) may be present in some brain areas. Overall, GalR1, GalR2, and GalR3 are distributed widely throughout the brain, correlate with widespread galanin binding, and colocalize with tyrosine hydroxylase in catecholaminergic brain areas.

show abstract

Section: Discussionmentioning

confidence: 58%

Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain

Hawes

Picciotto

2004

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…This and the previous hypothesis of an inhibitory effect of galanin on the NA cell bodies are consistent with the comparatively small FRS-induced release of NA in the GalOE͞D mouse, which has high galanin levels in the LC system due to the overexpression under the D␤H promoter (see ref. 16). This is also in agreement with increased galanin expression after stress (20,46,47).…”

Section: Discussionmentioning

confidence: 99%

Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test

Yoshitake

Wang

Kuteeva

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Basal and forced swimming (FS) stress-induced release of noradrenaline (NA) and serotonin (5-HT) were determined by in vivo microdialysis in the ventral hippocampus of mice overexpressing galanin under the platelet-derived growth factor B promoter (GalOE͞P) or under the dopamine ␤-hydroxylase promoter (GalOE͞D) (only NA). WT mice served as controls. Intraventricular infusion of galanin significantly reduced basal extracellular NA in WT mice and in GalOE͞P mice (albeit less so). Microdialysis sampling during a 10-min FS showed that NA and 5-HT release were elevated to 213% and 156%, respectively, in the GalOE͞P group, whereas in the WT group the increases were only 127% and 119%, respectively. The second (repeated) 10-min FS (RFS) caused a marked enhancement of NA and 5-HT release in the GalOE͞P mice to 344% and 275%, respectively. However, the RFS caused only a 192% increase of extracellular NA levels in the GalOE͞D mice. Pretreatment with the putative peptidergic galanin receptor antagonist M35 almost completely blocked the elevation of NA and 5-HT levels in the GalOE͞P after RFS. These results suggest that the NA and 5-HT hippocampal afferents in GalOE͞P mice are hypersensitive to both conditioned and unconditioned stressful stimuli, such as FS, and that this effect is mediated by galanin receptors. The present findings support a role of galanin in the regulation of release of NA and 5-HT, two neurotransmitters involved in mood control.microdialysis ͉ 5-hydroxytryptamine ͉ transgenic mice ͉ hippocampus ͉ affective disorders G alanin, a 29-aa (30 in human) neuropeptide (1), is widely distributed throughout the central nervous system (2, 3), where it coexists with several classic neurotransmitters (4). A similarly widespread distribution of galanin-like immunoreactivity (LI) in neurons and fibers was observed in the brain of the mouse (5). Three galanin receptor subtypes (GalR1-GalR3) have been cloned (see refs. 6 and 7), all belonging to the 7-transmembrane, G protein-coupled receptor superfamily. Both the noradrenaline (NA) neurons of locus coeruleus (LC) and the 5-hydroxytryptamine (5-HT) neurons of the dorsal raphe nucleus (DRN) (8) can synthesize galanin (4, 9-11). In the rat brain, galanin-LI can be observed in the terminal ramifications of the NA nerve terminals in cortical͞hippocampal areas (11). However, it has been more difficult to demonstrate galanin-LI in 5-HT terminals, suggesting a higher galanin expression in the NA than in the 5-HT system.Recently, mice with a targeted disruption of the galanin gene (12), or overexpressing galanin under the dopamine ␤-hydroxylase (D␤H) promoter (GalOE͞D) (13, † †) or the plateletderived growth factor B promoter (GalOE͞P) (14, ‡ ‡), have been generated and characterized with regard to several functional parameters (see refs. 15 and 16).In vivo microdialysis studies on rats have suggested that galanin plays an important role in modulation of 5-HT (17) and NA (18) neurotransmission, supporting a possible role of galanin in mood control, anxiety, and stress response...

show abstract

“…From the latter, a representative collection covering the different galanin fields and research groups is provided here for further reading Mazarati et al, 2001;Wiesenfeld-Hallin and Xu, 2001;Wynick et al, 2001;Zigmond, 2001;Crawley et al, 2002;Gundlach, 2002;Liu and Hökfelt, 2002;Vrontakis, 2002;Wynick and Bacon, 2002;Counts et al, 2003;Morilak et al, 2003;Ubink et al, 2003;Mazarati, 2004;Jacobowitz et al, 2004;Robinson, 2004;Lundstrom et al, 2005a;Hoyer and Bartfai, 2012). In addition, the proceedings of three of the galanin meetings Hökfelt and Crawley, 2005) and two multi-author reviews Tatemoto, 2008, 2010) have been published, with chapters by several authors who have been active in this field for many years.…”

Section: B History Of Galanin Researchmentioning

confidence: 99%

Physiology, Signaling, and Pharmacology of Galanin Peptides and Receptors: Three Decades of Emerging Diversity

Lang

Gundlach

Holmes

et al. 2015

Pharmacological Reviews

Self Cite

276

310

View full text Add to dashboard Cite

Galanin overexpressing transgenic mice

Cited by 48 publications

References 67 publications

Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain

Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain

Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test

Physiology, Signaling, and Pharmacology of Galanin Peptides and Receptors: Three Decades of Emerging Diversity

Contact Info

Product

Resources

About