Gametic differentiation in Chlamydomonas reinhardi: Cell cycle dependency and rates in attainment of mating competency

Gametogenesis in Liquid Cultures of Chlamydomonas eugametos

“…So, as in C. reinhardtii, gametogenesis in C. eugametos involves a gametogenic division (Kates & Jones, 1964;Schmeisser et al, 1973). Agglutination factor could be detected in the daughter cells prior to hatching.…”

“…Synchronous gametogenesis is accomplished by placing synchronously dividing vegetative cells in the mid G,-phase into nitrogen-free medium (Kates & Jones, 1964). Gametogenesis usually involves a mitotic division (Kates & Jones, 1964;Schmeisser et a/., 1973). Nitrogen depletion has also been reported to induce sexual differentiation in C. eugametos (Bernstein & Jahn, 1955), but there are, as far as we know, no reports of synchronous gametogenesis.…”

The effect of growth regimen, heat shock and intracellular starch storage on the mating efficiency ofChlamydomonas reinhardtii

“…The role of light in gametogenesis and mating has received considerable attention (Sager & Granick, 1954;Kates & Jones, 1964;Schmeisser, Baumgartel & Howell, 1973;Martin & Goodenough, 1975;Treier et al, 1989). Martin & Goodenough (1975) showed that gametes derived from wild type cells synchronized on a 12 h light-12 h dark cycle mated with 100% efficiency when light was extinguished 6h after suspension in N-free medium.…”

“…Several laboratories have examined factors which influence gametogenesis and mating in wild type Chlamydomonas grown both synchronously (Schmeisser, Baumgartel & Howell, 1973;Martin & Goodenough, 1975) and asynchronously (Sager & Granick, 1954;Schmeisser, Baumgartel & Howell, 1973;Martin & Goodenough, 1975). Collectively these studies have established that gametogenesis occurs in both liquid-grown and plate-grown cultures, that nitrogen deprivation is essential for gametogenesis in standard laboratory media, that the growth conditions of vegetative cells influence gametogenesis, and that continuous illumination is not essential for gametogenesis and mating.…”

Relationship between gametic differentiation and the cell cycle in the green alga Chlamydomonas eugametos

“…As stated by Harris (1989), gametogenesis in algae often appears to be triggered by adverse environmental conditions. In Chlamydomonas reinhardtii, nitrogen depletion has been reported to be the most important inducing factor (Sager & Granick, 1954;Kates & Jones, 1964;Martin & Goodenough, 1975;Siersma & Chiang, 1971 ;Schmeisser et al, 1973). Using synchronous cultures, Kates & Jones (1964) observed that cells resuspended in nitrogen-free medium produced abundant gametes only after a round of cell division.…”