Ganglioside 9-O-acetyl GD3 expression is upregulated in the regenerating peripheral nerve

Ribeiro-Resende, Victor Túlio; Oliveira-Silva, A.; Ouverney-Brandão, S.; Santiago, Marcelo F.; Hedin-Pereira, Cecília; Mendez-Otero, Rosália

doi:10.1016/j.neuroscience.2007.03.046

Cited by 20 publications

(18 citation statements)

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“…Current diagnostic approaches using electromyography 9 and magnetic resonance imaging cannot detect the degree of injury or the rate of nerve regeneration. 10 Experimental detection of peripheral nerve regeneration using immunohistochemical staining with specific antibodies for peripheral nerve tissue 11,12 and behavioral studies also have limitations in terms of clinical diagnosis. 13 Therefore, improvement of noninvasive methods is required for the evaluation of degeneration and regeneration of peripheral nerve tissue.…”

Section: Introductionmentioning

confidence: 99%

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

et al. 2013

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Abstract. Raman spectroscopy can be used for analysis of objects by detecting the vibrational spectrum using labelfree methods. This imaging method was applied to analysis of peripheral nerve regeneration by examining the sciatic nerve in vitro and in vivo. Raman spectra of intact nerve tissue had three particularly important peaks in the range 2800 − 3000 cm −1 . Spectra of injured sciatic nerves showed significant changes in the ratio of these peaks. Analysis of cellular spectra suggested that the spectrum for sciatic nerve tissue reflects the axon and myelin components of this tissue. Immunohistochemical analysis showed that the number of axons and the myelinated area were reduced at 7 days after injury and then increased by 28 days. The relative change in the axon to myelin ratio showed a similar initial increase, followed by a decrease at 28 days after injury. These changes correlated with the band intensity ratio and the changes in distribution of axon and myelin in Raman spectral analysis. Thus, our results suggest that label-free biochemical imaging with Raman spectroscopy can be used to detect turnover of axon and myelin in peripheral nerve regeneration. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionmentioning

confidence: 99%

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

et al. 2013

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“…By confocal imaging, most of the GD3 immunopositivities are diffusely localized in the presynaptic axonal area by the involvement of Schwann cells (Ogawa‐Goto et al, 1992; Ribeiro‐Resende et al, 2007). Some of the GD3 immunoreactivities, however, are condensed in a restricted area on the NMJs (Fig.…”

Section: Discussionmentioning

confidence: 99%

Novel anti‐idiotype antibody therapy for lipooligosaccharide‐induced experimental autoimmune neuritis: Use relevant to Guillain‐Barré syndrome

Usuki

Taguchi

Thompson

et al. 2010

J of Neuroscience Research

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Campylobacteriosis is a frequent antecedent event in Guillain-Barré syndrome (GBS), inducing high-titer serum antibodies for ganglioside antigens in the peripheral nervous system (PNS). Molecular mimicry between the lipooligosaccharide (LOS) component of Campylobacter jejuni and human peripheral nerve gangliosides is believed to play an important role in the pathogenesis of GBS. Conventional treatment strategies for patients with GBS include plasmapheresis, intravenous immunoglobulin (IVIG), and immunosuppression, which are invasive or relatively ineffective. In this study, we used our animal model of GBS, in which Lewis rats were immunized with GD3-like LOS isolated from C. jejuni. The animals developed anti-GD3 ganglioside antibodies and manifested neuromuscular dysfunction. To develop novel therapeutic strategies, we treated the animals by intraperitoneal administration of an anti-GD3 antiidiotype monoclonal antibody (BEC2) that specifically interacts with the pathogenic antibody. The treated animals had a remarkable reduction of anti-GD3 antibody titers and improvement of motor nerve functions. The results suggest that ganglioside mimics, such as antiidiotype antibodies, may be powerful reagents for therapeutic intervention in GBS by neutralizing specific pathogenic antiganglioside antibodies. Keywordsganglioside; Campylobacter jejuni; Guillain-Barré; syndrome; lipooligosaccharide; GD3 ganglioside; anti-idiotype antibody Guillain-Barré syndrome (GBS) is an immune-mediated peripheral neuropathy characterized by neuromuscular weakness and frequently accompanied by flaccid paralysis and may occasionally lead to death. The major pathological hallmarks involve demyelination, axonal degeneration, and/or impairment of neurotransmission by ion channel blockage (Rinaldi and Willison, 2008;van Doorn et al., 2008;Kaida et al., 2009 immune responses triggered by an infectious agent or vaccination allow disease development and the underlying pathogenetic mechanisms (Langmuir et al., 1984;Kuwabara, 2004;Souayah et al., 2007). The most commonly identified microbial agents are Campylobacter jejuni (C. jejuni), Haemophilus influenzae, cytomegalovirus (CMV), Epstein-Barr virus, and Mycoplasma pneumoniae (Hughes et al., 1999;Hadden et al., 2001;Sivadon-Tardy et al., 2006. A preceding infectious event and patient-related host factors also seem to be related to certain subtypes of GBS and may affect the severity of the disease (Geleijns et al., 2005;Caporale et al., 2006;Yuki, 2007). C. jejuni infection frequently induces antiganglioside antibodies in the patient's serum (Yuki et al., 1990;Usuki et al., 2006b). Thus, despite the possibility of other pathogenic mechanisms, an antibodymediated process is one of the major insults to the nerve, causing both conduction block and velocity loss and the ensuing clinical symptoms (Rinaldi and Willison, 2008;van Doorn et al., 2008;Kaida et al., 2009).The etiology of GBS has been not been fully clarified; one possibility is based on molecular mimicry and cross-reacting antiglycolip...

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“…The pro-survival role of OAcGD3 has been highlighted by the upregulation of OAcGD3 in the brain. Ribeiro-Resende and coworkers reported that OAcGD3 is upregulated in regenerating peripheral nerve fibers, suggesting that OAcGD3 is involved in neuronal growth and axon regeneration [78]. Furthermore, O-acetylation of GD3 suppresses the pro-apoptotic role of GD3 by relocating it from the mitochondria in acute lymphoblastic leukemia [79,80].…”

Section: O-acetylated Gangliosides In Cancersmentioning

confidence: 99%

O-acetylated Gangliosides as Targets for Cancer Immunotherapy

Cavdarli

Delannoy

Groux-Degroote

2020

Cells

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O-acetylation of sialic acid residues is one of the main modifications of gangliosides, and modulates ganglioside functions.O-acetylation of gangliosides is dependent on sialyl-O-acetyltransferases and sialyl-O-acetyl-esterase activities. CAS1 Domain-Containing Protein 1 (CASD1) is the only human sialyl-O-acetyltransferases (SOAT) described until now. O-acetylated ganglioside species are mainly expressed during embryonic development and in the central nervous system in healthy adults, but are re-expressed during cancer development and are considered as markers of cancers of neuroectodermal origin. However, the specific biological roles of O-acetylated gangliosides in developing and malignant tissues have not been extensively studied, mostly because of the requirement of specific approaches and tools for sample preparation and analysis. In this review, we summarize our current knowledge of ganglioside biosynthesis and expression in normal and pathological conditions, of ganglioside O-acetylation analysis and expression in cancers, and of the possible use of O-acetylated gangliosides as targets for cancer immunotherapy. a potential functional significance of ganglioside O-acetylation in cancer. The use of O-acetylated gangliosides as targets for cancer immunotherapy is also discussed. Ganglioside Structures and BiosynthesisGangliosides are acidic GSL containing from one to five sialic acids residues. They are mainly, but not exclusively, derived from ganglio-series GSL (GgCer) that result from the substitution of a ceramide (Cer) by the tetrasaccharide core Galβ1-3GalNAcβ1-4Galβ1-4Glcβ (Gg4). In different cell types and tissues, gangliosides are usually found as a mixture of di-, tri-and tetrasaccharide cores substituted by one or more sialic acid residues [1,5]. The biosynthesis of gangliosides takes place in the Golgi apparatus and starts by the transfer of sialic acid residues to lactosylceramide (LacCer) by specific sialyltransferases, i.e., the CMP-Neu5Ac: LacCer α2,3-sialyltransferase ST3Gal V (GM3 synthase) [6], the CMP-Neu5Ac: GM3 α2,8-sialyltransferase ST8Sia I (GD3 synthase) [7] and the CMP-Neu5Ac: GD3 α2,8-sialyltransferase ST8Sia V [8], that show high specificity toward glycolipid substrates [9]. Thus, LacCer, GM3, GD3 and GT3 are the precursors for 0-, a-, b-and c-series gangliosides, respectively (Figure 1).

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Ganglioside 9-O-acetyl GD3 expression is upregulated in the regenerating peripheral nerve

Cited by 20 publications

References 30 publications

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

Novel anti‐idiotype antibody therapy for lipooligosaccharide‐induced experimental autoimmune neuritis: Use relevant to Guillain‐Barré syndrome

O-acetylated Gangliosides as Targets for Cancer Immunotherapy

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