Ganglioside Microarrays for Toxin Detection

Abstract

Microarrays of lipids containing gangliosides were fabricated on surfaces coated with γ-aminopropylsilane. A fluorescence microarray scanner was used to detect the binding of toxins to these arrays. The specific binding of cholera and tetanus toxins to microspots containing GM1 and GT1b gangliosides, respectively, was demonstrated. These results suggest the possibility of using ganglioside microarrays for multiplexed toxin detection and the screening of compounds that can inhibit toxin binding.

Detection of bacterial toxins with monosaccharide arrays

“…Although recent approaches of developing carbohydrate-based sensors have employed gangliosides as receptors for protein toxins (Charych et al, 1996;Song and Swanson, 1999;Singh et al, 2000;Rowe-Taitt et al, 2000a;Fang et al, 2003;Ahn-Yoon et al, 2002), this approach has been less than fully successful at providing information about the specific carbohydrate moieties responsible for protein-carbohydrate interactions. As a result, there is limited understanding of these interactions and use in sensor development as well as toxicity and drug discovery studies.…”

Corrigendum to “The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome” [Curr Opin Chem Biol 18 (2014) 87–94]

“…Others have used vesicles or liposomes non-covalently [44,45] or covalently [46] immobilized on functionalized surfaces, or by using supported lipid bilayers [47,48]. Another advance is development of microand nanoarrays of natural cell membranes and lipid domains enriched in particular gangliosides [49 ].…”

A novel immobilization strategy using oligonucleotide as linker for small molecule microarrays construction

“…It has been successfully used in discovering the ligands for a variety of protein targets (Kuruvilla et al, 2002;Uttamchandani et al, 2004;Koehler et al, 2003), screening the inhibitors of enzymes (Wang et al, 2006), and activity-based profiling of enzymes (Salisbury et al, 2002;Zhu et al, 2003;Uttamchandani et al, 2005b). It has also been effectively employed as analytical tools for protein identification (Reddy and Kodadek, 2005), toxin detection (Fang et al, 2003), and antibiotics residues analysis (Ye et al, 2008;Zuo and Ye, 2006). The immobilization of small molecules is critical in the SMM construction.…”