Gap Junction Expression in Equine Endometrium1

Brady, H.A.; Blanchard, T.L.; Evans, Joseph P.; Varner, D.D.; Bruemmer, J.E.; Day, W.; Schwab, C.A.; Risek, Boris; Gilula, Norton B.; Burghardt, Robert C.

doi:10.1093/biolreprod/52.monograph_series1.507

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Gap junctions are thought to constitute an important aspect in the integration of uterine functions during pregnancy (Burghardt and Fletcher, 1990). Although their role in equine uterine function has not been elucidated, Brady et al (1995) described their clear expression in equine endometrium during oestrus, dioestrus and early pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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Summary Reasons for performing study: A level of synchrony between embryo and uterine environment is essential for the establishment of pregnancy when performing embryo transfer. The ability to extend the acceptable degree of asynchrony would allow more efficient use of recipient mares. Objectives: To establish if administration of the prostaglandin synthetase inhibitor, meclofenamic acid, to asynchronous recipient mares could widen the acceptable window of asynchrony for embryo transfer. Hypothesis: The prostaglandin synthetase inhibitory action of meclofenamic acid may act to suppress luteolysis and thereby allow for a greater degree of asynchrony between donor and recipient mares. Methods: A total of 72 Grade 1 horse embryos were transferred nonsurgically into the uteri of recipient mares that had ovulated 2 (n = 20), 3 (n = 20), 4 (n = 16) or 5 (n = 16) days before the donor. Half of the mares in each group were treated orally with 1 g meclofenamic acid, beginning on Day 9 after ovulation and continuing for 7 days after embryo transfer. Results: Comparison of recipient:donor asynchrony between treated and untreated mares was: +2 days, 9/10 pregnancies vs. 8/10 (P = 1.00); by +3 days, 8/10 vs. 2/10 (P = 0.025); by +4 days, 5/8 vs. 1/8 (P = 0.121); and by +5 days 3/8 vs. 0/8 (P = 0.20). In 10/11 meclofenamic acid‐treated and 23/25 untreated recipient mares that failed to become pregnant, luteolysis occurred at the normal time (14–19 days) after ovulation. Conclusion: Treatment with meclofenamic acid supported the establishment of pregnancy in recipient mares that ovulated before the donors. However, meclofenamic acid appeared to provide this support in a manner other than by suppression of luteolysis. Potential relevance: Pregnancy rates can be increased in recipient mares that ovulate 3 days before the donor by administration of meclofenamic acid.

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Section: Discussionmentioning

confidence: 99%

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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“…In the present study this spot was present in 100% of the samples from susceptible mares but only in 68.2% of the resistant mares. GAP junctions, which provide contacting cells with a common pool of regulatory and informational molecules, are involved in the integration of uterine function in cycling and early pregnant mares (Brady et al 1995) and this communication may be important for the coordinated secretory activity of the endometrium. An increase on connexin activity may be related to a higher expression of other proteins observed in susceptible mares or to an impaired control in the secretions of the endometrial glands.…”

Section: Discussionmentioning

confidence: 99%

Persistent mating-induced endometritis susceptibility: the role of uterine secretion

Malschitzky¹,

Fiala²,

Esmeraldino³

et al. 2008

PHK

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This inflammatory reaction removes excess of semen and bacterial contamination inoculated at the time of breeding (Kotilainen et al. 1994, Fiala et al. 2007 SummaryThe aim of this study was to compare the protein profile of endometrial secretions from estrous mares resistant or susceptible to persistent post-mating endometritis (PPME). The hypothesis was that before insemination in susceptible mares the uterine environment is disturbed. Endometrial secretions from 17 estrous mares were collected before artificial insemination (AI), when a pre-ovulatory follicle and a characteristic ultrasonic uterine image from estrous was observed, using a regular vaginal tampon. Immediately after the remove of the tampon an endometrial biopsy was obtained. The biopsies were evaluated by immunohistochemistry. Another examination was performed 36-48h after AI to confirm ovulation and to detect intrauterine fluid accumulation (IUF). The mares were classified into 2 groups, according to the findings: "Resistant" (n = 11), no IUF was detected 36-48 h post AI and "Susceptible" (n = 6), mares presenting more than 2 mm of IUF 36-48 h after AI. An aliquot of endometrial secretions was centrifuged and the supernatant was transferred to cryovials for storage in liquid nitrogen until assay. The endometrial samples were recentrifuged and then submitted to 2D-PAGE using 12% acrylamide gels. Protein (100 mg) was loaded into the gels. Gels were stained in a 0.15% Comassie Brilliant Blue R-250. The Optiquant Acquisition & Analysis software was used to determine the relative protein content of the spots. The image analysis software identified 33 protein spots, with a molecular weight (MW) ranging from 15 to 105 kDa and isoeletric point (Ip) from 4.3 to 10.0. Twelve proteins showed higher relative protein content and eight proteins showed frequency differences in endometrial secretions from susceptible compared to resistant mares. Based on MW and Ip, the proteins may be related to inflammatory processes and uterine contractility. A higher (P = 0.07) estrogen and progesterone receptor staining intensity in stromal and luminal epithelium cells in susceptible mares was also observed. In conclusion, there is a difference in the uterine environment between resistant and susceptible mares to PPME, probably affecting the inflammatory response to spermatozoa. This difference can be related to ovarian steroid receptors expression.

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