Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus <i>Muscidifurax</i>

Bernier, Ulrich R.; Carlson, David A.; Geden, Christopher J.

doi:10.1016/s1044-0305(97)00288-2

Cited by 45 publications

(23 citation statements)

References 36 publications

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“…Mass spectra were obtained at 70 eV and the GC/MSD interface temperature was set at 280 8C. The identification of cuticular hydrocarbons was based upon EI mass spectra and literature data [21,23,24]. …”

Section: Chemical Analysismentioning

confidence: 99%

Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval

Zhu

et al. 2007

Forensic Science International

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Section: Chemical Analysismentioning

confidence: 99%

Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval

Zhu

et al. 2007

Forensic Science International

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“…CHCs have been used for species differentiation of some insects including parasitic wasps [12], phlebotomines [13,14], anophelines [15,16], culicids [17], triatomines [18,19] and the forensically important Diptera Calliphoridae [20-27] and Sarcophagidae [28]. …”

Section: Introductionmentioning

confidence: 99%

Effect of age on cuticular hydrocarbon profiles in adult Chrysomya putoria (Diptera: Calliphoridae)

Braga

Pinto

Queiroz

et al. 2016

Forensic Science International

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A species-specific complex mixture of highly stable cuticular hydrocarbons (CHCs) covers the external surface of all insects. Components can be readily analyzed by gas chromatography coupled to mass spectrometry (GC-MS) to obtain a cuticular hydrocarbon profile, which may be used as an additional tool for the taxonomic differentiation of insect species and also for the determination of the age and sex of adult and immature forms. We used GC-MS to identify and quantify the CHCs of female and male Chrysomya putoria (Wiedemann, 1818) (Diptera: Calliphoridae) from one to five days old. CHCs ranged from C21 to C35 for females and from C21 to C37 in males. Major compounds were the same for both sexes and were 2-MeC28, C29:1, n-C29, 15-,13-MeC29, 2-MeC30, C31:1, n-C31 and 15-,13-MeC31. The relative abundance of each component, however, varied with age. Cluster Analysis using Bray-Curtis measure for abundance showed that cuticular hydrocarbon profiles are a strong and useful tool for the determination of age in adult C. putoria.

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“…Even molecular techniques may not be used in most cases due to the natural decomposition of DNA, proteins and enzymes (Ye et al, 2007). Several authors have demonstrated the usefulness of cuticular hydrocarbons for identifying insect species, including parasitic wasps (Bernier et al, 1997), phlebotomines (Bejarano et al, 2003; Mahamat and Hassanali, 1998), anophelines (Anyanwu et al, 2000, 2001), culicids (Horne and Priestmann, 2002) and triatomines (Calderón-Fernández et al, 2005a,b). The external surface of all insects is covered by a layer of species-specific cuticular lipids.…”

Section: Introductionmentioning

confidence: 99%

Cuticular hydrocarbons as a tool for the identification of insect species: Puparial cases from Sarcophagidae

et al. 2013

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The external surface of all insects is covered by a species-specific complex mixture of highly stable, very long chain cuticular hydrocarbons (CHCs). Gas chromatography coupled to mass spectrometry was used to identify CHCs from four species of Sarcophagidae, Peckia (Peckia) chrysostoma, Peckia (Pattonella) intermutans, Sarcophaga (Liopygia) ruficornis and Sarcodexia lambens. The identified CHCs were mostly a mixture of n-alkanes, monomethylalkanes and dimethylalkanes with linear chain lengths varying from 23 to 33 carbons. Only two alkenes were found in all four species. S. lambens had a composition of CHCs with linear chain lengths varying from C23 to C33, while the other three species linear chain lengths from 24 to 31 carbons. n-Heptacosane, n-nonacosane and 3-methylnonacosane, n-triacontane and n-hentriacontane occurred in all four species. The results show that these hydrocarbon profiles may be used for the taxonomic differentiation of insect species and are a useful additional tool for taxonomic classification, especially when only parts of the insect specimen are available.

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Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax

Cited by 45 publications

References 36 publications

Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval

Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval

Effect of age on cuticular hydrocarbon profiles in adult Chrysomya putoria (Diptera: Calliphoridae)

Cuticular hydrocarbons as a tool for the identification of insect species: Puparial cases from Sarcophagidae

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