Gastric Adenocarcinoma Predictive Long Intergenic Non-Coding RNA Promotes Tumor Occurrence and Progression in Non-Small Cell Lung Cancer via Regulation of the miR-661/eEF2K Signaling Pathway

Gu, Haiting; Chen, Junfeng; Song, Yukang; Shao, Hai-yan

doi:10.1159/000495831

Cited by 17 publications

(18 citation statements)

References 25 publications

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“…Annexin V‐FITC/PI Apoptosis Analysis: To assess the percentage of apoptotic Hela cells after treated with nano 1 – 3 , the Annexin V‐FITC/PI staining assay (Abcam Apoptosis Detection Kit; ab214663) was conducted according with the manufacturer's instruction . In brief, the Hela cells were seeded in 6‐well plates (1 × 10 6 cells per well) at 37 °C, 5 % CO 2 overnight.…”

Section: Methodsmentioning

confidence: 99%

Retracted: Synthesis, Crystal Structures, and Anti‐cervical Cancer Activity Evaluation of Three Trinuclear Transition Metal(II) Complexes with N,O‐Donor Schiff Base Ligand

Luo

Zhu

2019

Zeitschrift anorg allge chemie

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A new bi‐nucleating Schiff base ligand, 2‐(((3‐(dimethylamino)propyl)imino)methyl)‐6‐methoxyphenol (HL1) was prepared by a one‐pot condensation reaction, which was further used in the construction of three trinuclear Schiff base transition metal(II) complexes [Cu3(L1)2(OH)2(H2O)2](NO3)2 (1), [Co3(L1)2(OH)2(H2O)2](NO3)2 (2), and [Cu3(L1)2(N3)4] (3). Furthermore, a green hand grinding technique was implemented to reduce the particle size of the coordination complexes to generate the nanoscale compounds. The SEM studies reveal the formation of square and spherical particles for nano 1 and 2, and nanorod for nano 3. In addition, the anti‐proliferation activity of nano 1–3 was detected on the human cervical cancer Hela cells with CCK‐8 assay. The cell viability curves and IC50 values indicated that only nano 1 has anti‐proliferation activity on Hela cells. To further investigate the mechanism of nano 1 induced Hela cell death, the Annexin V‐FITC/PI double staining assay, western blot assay, and ROS level detection was conducted.

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Section: Methodsmentioning

confidence: 99%

Retracted: Synthesis, Crystal Structures, and Anti‐cervical Cancer Activity Evaluation of Three Trinuclear Transition Metal(II) Complexes with N,O‐Donor Schiff Base Ligand

Luo

Zhu

2019

Zeitschrift anorg allge chemie

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“…1F). This is consistent with findings in cancer cells, in which GAPLINC is mainly localized to the cytoplasm (9). To ensure GAPLINC is noncoding, we performed polysome profiling, a method used to analyze whether a gene is actively translated into protein.…”

Section: Significancesupporting

confidence: 85%

A conserved long noncoding RNA, GAPLINC, modulates the immune response during endotoxic shock

Vollmers

Covarrubias

Kuang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Recent studies have identified thousands of long noncoding RNAs (lncRNAs) in mammalian genomes that regulate gene expression in different biological processes. Although lncRNAs have been identified in a variety of immune cells and implicated in immune response, the biological function and mechanism of the majority remain unexplored, especially in sepsis. Here, we identify a role for a lncRNA—gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC)—previously characterized for its role in cancer, now in the context of innate immunity, macrophages, and LPS-induced endotoxic shock. Transcriptome analysis of macrophages from humans and mice reveals that GAPLINC is a conserved lncRNA that is highly expressed following macrophage differentiation. Upon inflammatory activation, GAPLINC is rapidly down-regulated. Macrophages depleted of GAPLINC display enhanced expression of inflammatory genes at baseline, while overexpression of GAPLINC suppresses this response. Consistent with GAPLINC-depleted cells, Gaplinc knockout mice display enhanced basal levels of inflammatory genes and show resistance to LPS-induced endotoxic shock. Mechanistically, survival is linked to increased levels of nuclear NF-κB in Gaplinc knockout mice that drives basal expression of target genes typically only activated following inflammatory stimulation. We show that this activation of immune response genes prior to LPS challenge leads to decreased blood clot formation, which protects Gaplinc knockout mice from multiorgan failure and death. Together, our results identify a previously unknown function for GAPLINC as a negative regulator of inflammation and uncover a key role for this lncRNA in modulating endotoxic shock.

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“…Recently, increasing studies have indicated the essential functions of lncRNAs in various human cancers (Bhan, Soleimani, & Mandal, 2017;Gu, Chen, Song, & Shao, 2018), which implies lncRNAs might be potential targets for tumor therapy. LncRNA CACNA1G-AS1 is an antisense RNA of CACNA1G.…”

Section: Discussionmentioning

confidence: 99%

LncRNA CACNA1G‐AS1 facilitates hepatocellular carcinoma progression through the miR‐2392/C1orf61 pathway

Yang

et al. 2019

Journal Cellular Physiology

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Emerging studies have indicated that long noncoding RNAs (lncRNAs) possess various functions in initiating human cancers. However, the role of lncRNAs in hepatocellular carcinoma (HCC) still remains ill understood. In this study, we sought to investigate the role of lncRNA CACNA1G‐AS1 in HCC progression. Through bioinformatics analysis, we found that CACNA1G‐AS1 expression was significantly upregulated in HCC tissues compared with that in the adjacent normal tissues. Moreover, CACNA1G‐AS1 upregulation indicated poor prognosis in HCC patients. Knockdown of CACNA1G‐AS1 attenuated the proliferation, migration, and invasion of HCC cells. Additionally, decreased expression of CACNA1G‐AS1 prevented epithelial–mesenchymal transition. In vivo assay also showed that CACNA1G‐AS1 silencing HCC cells have smaller tumor volumes and weights. Further investigations demonstrated that CACNA1G‐AS1 worked as a competing endogenous RNA to bind microRNA‐2392 (miR‐2392) and thereby alleviate the repression of the downstream target C1orf61. Collectively, CACNA1G‐AS1 promotes HCC progression through regulating the miR‐2392/C1orf61 pathway.

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Gastric Adenocarcinoma Predictive Long Intergenic Non-Coding RNA Promotes Tumor Occurrence and Progression in Non-Small Cell Lung Cancer via Regulation of the miR-661/eEF2K Signaling Pathway

Cited by 17 publications

References 25 publications

Retracted: Synthesis, Crystal Structures, and Anti‐cervical Cancer Activity Evaluation of Three Trinuclear Transition Metal(II) Complexes with N,O‐Donor Schiff Base Ligand

Retracted: Synthesis, Crystal Structures, and Anti‐cervical Cancer Activity Evaluation of Three Trinuclear Transition Metal(II) Complexes with N,O‐Donor Schiff Base Ligand

A conserved long noncoding RNA, GAPLINC, modulates the immune response during endotoxic shock

LncRNA CACNA1G‐AS1 facilitates hepatocellular carcinoma progression through the miR‐2392/C1orf61 pathway

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