Gastrin-Releasing Peptide Phase-Shifts Suprachiasmatic Nuclei Neuronal Rhythms<i>In Vitro</i>

McArthur, Angela J.; Coogan, Andrew N.; Ajpru, Supaporn; Sugden, David; Biello, Stephany M.; Piggins, Hugh D.

doi:10.1523/jneurosci.20-14-05496.2000

Cited by 95 publications

(81 citation statements)

References 45 publications

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“…night (Piggins and Rusak, 1993;Piggins et al, 1994Piggins et al, , 1995Albers et al, 1995;McArthur et al, 2000). It has also been shown that the phase-shifting effects of a gastrin-releasing peptide injection can be blocked by the application of the gastrin-releasing peptide antagonist [D-F5Phe6,D-Ala11]BN(6 -13)OMe (Piggins et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Phenotype Matters: Identification of Light-Responsive Cells in the Mouse Suprachiasmatic Nucleus

et al. 2004

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Section: Discussionmentioning

confidence: 99%

Phenotype Matters: Identification of Light-Responsive Cells in the Mouse Suprachiasmatic Nucleus

et al. 2004

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“…Intercellular signaling through VIP and its receptor VPAC2 is critical in maintaining the synchrony of SCN cellular oscillators and circadian function within the SCN (Aton et al 2005;Maywood et al 2006). In addition, GRP cells are retinorecipient, they communicate with cells in the shell region through GRP receptors, and they function to keep SCN neurons synchronized during entrainment (McArthur et al 2000;Aida et al 2002;Karatsoreos et al 2004;). …”

Section: Function Of Scn Subregionsmentioning

confidence: 99%

Exploring Spatiotemporal Organization of SCN Circuits

Yan

Karatsoreos

LeSauter

et al. 2007

Cold Spring Harbor Symposia on Quantitative Biology

105

111

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Suprachiasmatic nucleus (SCN) neuroanatomy has been a subject of intense interest since the discovery of the SCN's function as a brain clock and subsequent studies revealing substantial heterogeneity of its component neurons. Understanding the network organization of the SCN has become increasingly relevant in the context of studies showing that its functional circuitry, evident in the spatial and temporal expression of clock genes, can be reorganized by inputs from the internal and external environment. Although multiple mechanisms have been proposed for coupling among SCN neurons, relatively little is known of the precise pattern of SCN circuitry. To explore SCN networks, we examine responses of the SCN to various photic conditions, using in vivo and in vitro studies with associated mathematical modeling to study spatiotemporal changes in SCN activity. We find an orderly and reproducible spatiotemporal pattern of oscillatory gene expression in the SCN, which requires the presence of the ventrolateral core region. Without the SCN core region, behavioral rhythmicity is abolished in vivo, whereas low-amplitude rhythmicity can be detected in SCN slices in vitro, but with loss of normal topographic organization. These studies reveal SCN circuit properties required to signal daily time.

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“…CalB (Bryant et al, 2000) and GRP (Aioun et al, 1998) cells receive retinal terminals and presentation of a light pulse during the night induces c-Fos in about 80% of the CalB cells (Silver et al, 1996), 78% of the GRP cells and 60% of the VIP cells (Aioun et al, 1998). It is also known that administration of GRP (McArthur et al, 2000) or coadministration of VIP, peptide histidine isoleucine and GRP (Albers et al, 1991) induce phase-dependent resetting of the SCN that resembles the lightinduced phase response curve. Finally, administration of CalB antisense blocks lightinduced phase shifts (Hamada et al, 2000).…”

Section: Colocalization Of Peptides In the Calb Subnucleusmentioning

confidence: 99%

Calbindin-D28K cells selectively contact intra-SCN neurons

et al. 2002

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Calbindin-D 28K -immunoreactive cells are tightly packed within a discrete region of the caudal aspect of the suprachiasmatic nuclei of hamsters. These cells receive direct retinal input and are Fos-positive in response to a light pulse. Knowledge of their afferent and efferent connections is necessary to understand suprachiasmatic nucleus organization. The first aim of the present study is to identify interconnections between calbindin and other peptidergic cells of the suprachiasmatic nuclei, using epi-and confocal microscopy and intra-suprachiasmatic nucleus tract tracing. The results indicate that essentially all calbindin cells receive numerous appositions from vasoactive intestinal polypeptide (VIP), neuropeptide Y and serotonin fibers and that most receive appositions from gastrin releasing peptide (GRP) and cholecystokinin (CCK) fibers. Reciprocal connections are seen from VIP, GRP and CCK cells but surprisingly, not from dorsomedial vasopressin cells. Injection of biotinylated dextran amine into the suprachiasmatic nucleus indicates that the ventrolateral suprachiasmatic nucleus projects to the entire nucleus, while the dorsal and medial regions of the suprachiasmatic nucleus project densely to most of the nucleus, except to the calbindin region. Analysis of colocalization of the peptides in the calbindin cell region shows that 91% of the substance P cells, 42% of the GRP cells and 60% of the VIP cells in the calbindin subnucleus coexpress calbindin-D 28K .Our results reveal a highly specialized topographical organization of connections among suprachiasmatic nucleus cells. Keywords suprachiasmatic nucleus; calbindin; vasopressin; confocal microscopy; tract tracingThe suprachiasmatic nuclei (SCN) are the locus of the brain clock regulating circadian rhythms in mammals (reviewed in Silver and Moore, 1998). In rodents, these paired hypothalamic nuclei are each composed of about 8000 neurons that are heterogeneous in their content of neuronal antigens (van den Pol, 1980;van den Pol and Tsujimoto, 1985). Some of the neuropeptides that have been identified to date in the rodent SCN are vasopressin (AVP), vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine, gastrin releasing peptide (GRP), sub-stance P (SP), cholecystokinin (CCK) and somatostatin. We have reported that a subregion in the caudal SCN is characterized by the (Silver et al., 1996). These cells are densely packed, receive direct retinal synaptic input (Bryant et al., 2000) and are responsive to photic stimuli, with about 80% of the cells showing Fos-like immunoreactivity (ir) following a light pulse given during subjective night (Silver et al., 1996). The foregoing evidence indicates these CalB cells are on the input pathway for photic stimuli reaching the SCN.The function of this subregion of the SCN has been examined in hamsters. Animals with lesions that destroyed the CalB subnucleus, but spared other compartments of the SCN, lost their locomotor activity rhythm. Animals with partial bilateral SCN lesions sparing part ...

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Gastrin-Releasing Peptide Phase-Shifts Suprachiasmatic Nuclei Neuronal RhythmsIn Vitro

Cited by 95 publications

References 45 publications

Phenotype Matters: Identification of Light-Responsive Cells in the Mouse Suprachiasmatic Nucleus

Phenotype Matters: Identification of Light-Responsive Cells in the Mouse Suprachiasmatic Nucleus

Exploring Spatiotemporal Organization of SCN Circuits

Calbindin-D28K cells selectively contact intra-SCN neurons

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