Gastrointestinal microbial diversity and short-chain fatty acid production in pigs fed different fibrous diets with or without cell wall-degrading enzyme supplementation

Zhang, Y.J.; Liu, Q.; Zhang, W.M.; Zhang, Z.J.; Wang, W.L.; Zhuang, Songlin

doi:10.1016/j.livsci.2017.11.017

Cited by 26 publications

(19 citation statements)

References 70 publications

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“…(amylolytic bacteria) tended to be decreased in the ileum and were significantly decreased in the colon of pigs fed EB-supplemented diets compared with unsupplemented diets. These data appear to be due to the accelerated removal of starch by EB (Bedford and Cowieson, 2012;Zhang et al, 2018). In accordance with the current experiment, Smith et al (2010) showed that xylanase and ß-glucanase supplementation decreased Lactobacilli populations in the ileum compared with treatment without enzymes.…”

Section: Discussionsupporting

confidence: 89%

“…Xu et al (2016) speculated that the decrease in Lactobacillus on day 8 postweaning in the stomach and ileum of piglets supplemented with butyrate may not negatively affect gut health, as microbial diversity was increased and Lactobacillus was still the most predominant genus. Furthermore, pigs fed EB-supplemented diets tended to have reduced colonic Enterobacteriaceae, indicating less abundance of pathogenic bacteria, which agreed with Zhang et al (2014) and Zhang et al (2018). Collectively, the decreased Lactobacillus spp.…”

Section: Discussionsupporting

confidence: 62%

See 1 more Smart Citation

Exogenous carbohydrases added to a starter diet reduced markers of systemic immune activation and decreasedLactobacillusin weaned pigs1

Schmitz‐Esser

Loving

et al. 2018

Journal of Animal Science

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 62%

Exogenous carbohydrases added to a starter diet reduced markers of systemic immune activation and decreasedLactobacillusin weaned pigs1

Schmitz‐Esser

Loving

et al. 2018

Journal of Animal Science

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“…Fernandes et al (2014) reported that SCFAs are absorbed in the colon and that the SCFA content in the feces is lower compared with that in the cecal digesta. Zhang Y.J. et al (2017) showed that Lactobacillus and Megasphaera are affected by the fiber source.…”

Section: Discussionmentioning

confidence: 99%

Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

et al. 2019

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Lactobacillus has a positive effect on the host intestinal microbiota. In piglets, dietary supplementation with Lactobacillus affects general health and plays an important role in nutrient digestion and fermentation. However, this association requires further investigation. Here, we studied newborn piglets from 12 litters. The nursed piglets were given a creep feed beginning on day 10 post-partum and weaned at day 30. Piglets were fed either a control basic diet or a diet including supplementation with Lactobacillus reuteri ZLR003 at 6.0 × 10 6 CFU/g feed. At day 30 and 60, feces samples were taken and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. At day 60, feces samples and serum samples were also taken and used to measure the short chain fatty acids (SCFAs) and to detect long chain fatty acids (LCFAs) and free amino acids (FAAs), respectively. The results revealed that L. reuteri ZLR003 could improve piglet fecal microbiota composition, especially at the end of weaned period. The concentrations of lactic acid and butyric acid in feces were higher, and acetic acid concentration was lower in the L. reuteri ZLR003 group compared with the control group ( P < 0.05). The serum polyunsaturated fatty acids C18:2n6c, C18:3n3, C20:4n6, and C22:6n3 were significantly higher ( P < 0.05), as were the serum FAAs Gly, Ala, Val, Iso, Asn, Asp, Glu, Met, Phe, and Leu ( P < 0.05), in the L. reuteri group compared with the control group. A correlation analysis revealed that the genera Ruminococcaceae _UCG-010 and Ruminococcaceae _UCG-014 had a negative correlation with the SCFAs content in feces, the genus Prevotella _9 had a higher positive correlation with C18:2n6c, and the genera Megasphaera and Mitsuokella had a more positive significant effect on the serum FAAs content in weaned piglets in the L. reuteri ZLR003 group compared with the control group. In conclusion, L. reuteri ZLR003 influenced the fecal microbiota composition of piglets, and its effects were related to the metabolism of SCFAs, LCFAs, and FAAs. Our findings will help facilitate the application of Lactobacillus strains in pig production.

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“…The composition of the microbiota can be affected by several factors such as age, diet, environment and host genetics. Few studies have examined the changes in microbial communities in response to various diets in pigs [51, 52]. In this study, we examined microbial community changes in young pigs fed a basal diet supplemented with multi-enzyme complex by using NGS.…”

Section: Discussionmentioning

confidence: 99%

Dietary multi-enzyme complex improves In Vitro nutrient digestibility and hind gut microbial fermentation of pigs

et al. 2019

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This study was conducted in two stages to investigate the potential of multi-enzyme supplementation on the nutrient digestibility, growth performance, and gut microbial composition of pigs. In stage 1, effects of multi-enzyme complex (xylanase, α-amylase, β-glucanase, and protease) supplementation on the ileal and total tract dry matter (DM) digestibility of feed-stuffs were investigated with in vitro two-stage and three-stage enzyme incubation methods. A wide range of feed ingredients, namely, corn meal, wheat meal, soybean meal, fish meal, Oriental herbal extract, Italian rye-grass (IRG) and peanut hull were used as substrates. Supplementation of the multi-enzyme complex increased ( P < 0.05) the digestibility of the Oriental herbal extract and corn meal. In stage 2, in vivo animal studies were performed to further investigate the effects of the dietary multi-enzyme complex on the nutrient utilization, growth performance, and fecal microbial composition of pigs. A total of 36 weaned pigs were fed corn- and soybean meal-based diets without (control) and with the multi-enzyme complex (treatment) for 6 weeks. Fecal samples were collected from 12 pigs to analyze the microbial communities by using DNA sequencing and bioinformatics tools. Multi-enzyme supplementation had no effect on apparent digestibility of nutrients and growth performance of pigs compared to control. Taxonomic analysis of the fecal samples indicated that the bacteria in both control and treatment samples predominantly belonged to Firmicutes and Bacteroidetes . In addition, the proportion of the phylum Firmicutes was slightly higher in the treatment group. At the genus level, the abundance of Treponema and Barnesiella increased in the treatment group; whereas the numbers ofthe genera including Prevotella , Butyricicoccus , Ruminococcus and Succinivibrio decreased in the treatment group. These results suggest that multi-enzyme supplementation with basal diets have the potential to improve nutrient digestibility and modify microbial communities in the hind-gut of pigs.

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Gastrointestinal microbial diversity and short-chain fatty acid production in pigs fed different fibrous diets with or without cell wall-degrading enzyme supplementation

Cited by 26 publications

References 70 publications

Exogenous carbohydrases added to a starter diet reduced markers of systemic immune activation and decreasedLactobacillusin weaned pigs1

Exogenous carbohydrases added to a starter diet reduced markers of systemic immune activation and decreasedLactobacillusin weaned pigs1

Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

Dietary multi-enzyme complex improves In Vitro nutrient digestibility and hind gut microbial fermentation of pigs

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