Gel Beads Composed of Chitosan and Polyacids and Their Blood Compatibility

Abstract

Chitosan gel beads were prepared as a model for new chitosan materials and their blood compatibility was evaluated in vitro in human pool plasma and in human whole blood. The one-stage method for the preparation of monolithic beads consisted of performing a template polymerization of 2-acryloylamido-2-methylpropanesulfonic acid (AMPS) or copolymerization of AMPS and acrylic acid. Monolithic beads of semi-interpenetrating networks chitosan/copolymer were prepared in the presence of a cross-linking agent. The equilibrium degree of swelling of the beads was dependent on the composition. Reservoir-type beads consisting of a chitosan core and a coating of polyelectrolyte complex with anticoagulant activity and chitosan were also prepared. The reservoir-type beads and the monolithic beads showed improved compatibility with blood in comparison with the uncoated chitosan beads.

Electrospun Chitosan‐Coated Fibers of Poly(L‐lactide) and Poly(L‐lactide)/Poly(ethylene glycol): Preparation and Characterization

“…Often, the behavior of polymer materials on contact with blood is evaluated by SEM study of blood cell adhesion after in vitro contact of the material with blood 6, 28, 29. In the present study, the effect of chitosan coating and the presence of PEG in the fibers on the behavior of PLLA and PLLA/PEG mats on contact with blood were estimated.…”

Polyelectrolyte Complexes Based on (Quaternized) Poly[(2‐dimethylamino)ethyl methacrylate]: Behavior in Contact with Blood

“…Recently, some of us have shown that PAMPSNa possesses anticoagulant activity comparable to that of the natural anticoagulant heparin14 and its complex formation with haemostatic polymers leads to polymer materials with improved blood compatibility 11. We have studied the complex formation between (quaternized) PDMAEMA and PAMPSNa 22.…”

“…The time for clot formation was determined after mixing the plasma with the corresponding reagent (STA Neoplastin® Plus, STA Thrombin, STA APTT Kaolin, STA Fibrinogen, or STA Antithrombin III). The data obtained from the clottability assay was processed using the following equations in accordance with already reported studies:11, 23 where PT c , TT c , APTT c , Fibrinogen c , and ATIII c are the values of the haemostatic parameters in the presence of CECh or (quaternized) PDMAEMA and PT 0 , TT 0 , APTT 0 , Fibrinogen 0 , and ATIII 0 are those of the controls in the absence of polymers. The concentration of the polymers was in the range of 8–16 mg · mL −1 in the total volume.…”

“…One of the strategies developed to improve the blood compatibility of DMAEMA‐based copolymers is the introduction of a poly(ethylene oxide) block in their structure 1, 3. Another approach is the formation of polyelectrolyte complexes (PECs) with polyanions,10, 11 although in some cases increased toxicity has been reported 12…”

Novel Thermally Sensitive pH-dependent Chitosan/ Carboxymethyl Cellulose Hydrogels

“…It has been shown to be biocompatible and has been used successfully in a range of applications from drug delivery [13,15] to cell implantation in vivo [16,17]. Carboxymethyl cellulose (CMC) is also a derivative of cellulose, which is often used as thickener and additive in the food and chemical industries.…”