Gelation dynamics and gel structure of fibrinogen

Kubota, Kenji; Kogure, Hiroyuki; Masuda, Yuka; Toyama, Yoshiharu; Kita, R.; Takahashi, Atsuo; Kaibara, Makoto

doi:10.1016/j.colsurfb.2004.02.017

Cited by 30 publications

(19 citation statements)

References 14 publications

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“…At high thrombin concentrations, tight networks are formed with more fiber bundles with finer and thinner fibers. As the thrombin concentration is decreased, the average fiber bundle size increases and the gel becomes more porous [17,19].…”

Section: Introductionmentioning

confidence: 99%

“…While ionic strength and hydrogen ion concentration are important, clotting time is a dominant parameter in the determination of structure [16][17]. Increases in thrombin concentration are associated with faster gelation times [18][19], as well as characteristics of the gel microstructure such as fiber size and porosity. At high thrombin concentrations, tight networks are formed with more fiber bundles with finer and thinner fibers.…”

Section: Introductionmentioning

confidence: 99%

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Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels

2007

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Fibrin is a biopolymer that has been used in a variety of biomaterial, cell delivery and tissue engineering applications. The enzyme thrombin catalyzes the formation of fibrin microfibrils, which form a three-dimensional mesh in which cells can be directly embedded at the time of gel formation. In this study, fibrin hydrogels containing vascular smooth muscle cells were created using varying concentrations of thrombin. Over 7 days in culture, all gels decreased in volume as the fibrin matrix compacted, and the degree of gel compaction increased as thrombin concentration decreased. The material modulus and ultimate tensile stress of the gels also increased with decreasing thrombin concentration. Addition of thrombin to similar constructs made using collagen Type I did not show an effect on gel compaction or mechanical properties, suggesting that these effects were a result of thrombin's action on fibrin polymerization, and not cellular functions. Cell proliferation in fibrin hydrogels was not significantly affected by thrombin addition. Matrix examination using scanning electron microscopy showed increasing fibrin fiber diameters as thrombin concentration decreased. Confocal microscopic imaging of the actin cytoskeleton showed that cell morphology on twodimensional substrates of fibrin showed marked changes, with higher thrombin concentrations producing cells with longer cellular projections. However, these morphological changes were not as apparent in cells embedded in three-dimensional (3-D) matrices, in which cells exhibited a similar morphology independent of thrombin concentration. These results relate features of the matrix and cellular components of 3-D fibrin constructs to mechanical properties, and contribute to the understanding of structure-function relationships in cell-seeded, 3-D protein hydrogels.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels

2007

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“…The blood coagulation cascade can be activated by a specific stimulus such as injury or exposure to a thrombogenic surface. A key enzyme required for the activation of the cascade is thrombin triggering the polymerization of fibrinogen into fibrin fibrils [26,27]. Thrombin-antithrombin (TAT) complexes have been used as a surrogate marker for thrombin generation [28] and were used in this study as a marker for coagulation cascade activation.…”

Section: Introductionmentioning

confidence: 99%

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

et al. 2012

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“…In addition, studies in tissue engineering have reported that increased thrombin concentration modifies the microfibril structure of the fibrin gels, by decreasing the fiber bundling size and so decreasing the porosity of the gel, which may lead to reduced adhesion strength. 3,12,15) Maximum adhesion strength of autologous fibrin glue was reported with thrombin concentration of 50 units/ml, and a significant decrease with thrombin concentrations over 200 units/ml, suggesting inhibition of fibrin association as thrombin increases. 20) Our studies showed that maximum strength was acquired using 25 units/ml thrombin concentration, a slightly lower concentration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Thrombin Concentration on the Adhesion Strength and Clinical Application of Fibrin Glue-Soaked Sponge

Campos

Shimizu

Sugata

et al. 2013

Neurol. Med. Chir.(Tokyo)

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Fibrin glue-soaked gelatin sponge (FGGS) has been used for tissue sealing in neurosurgical practice, but too rapid clotting of fibrin glue occasionally prevents good fixation of FGGS. Dilution of thrombin may provide adequate manipulation time between mixing fibrinogen and thrombin on gelatin sponge and application into the tissue defects. The present study characterized the effect of thrombin dilution on the adhesion strength of FGGS and retrospectively assessed the clinical usage of the dilution for filling dead space or sealing arachnoid defect in 255 cases who underwent transsphenoidal surgery for the last 66 months. FGGS was prepared using three different concentrations of thrombin: 250 (standard), 50 (1:5 dilution), and 25 (1:10 dilution) units/ml, and incubated for three different periods (5, 20, and 60 seconds). FGGSs were applied over two adjacently positioned porcine skins placed on two metallic plates. The adhesion strength was evaluated by measuring maximum tensile strength during pulling out the sliding plate at a constant rate of displacement. The maximum adhesion strength was greater for FGGS with 1:10 diluted thrombin solution than for FGGS prepared with higher concentrations (p º 0.05). Adhesion strength did not decay for 20 seconds after the mixture. Only four of 255 cases (1.6%) required second reconstruction of sella floor due to the cerebrospinal fluid leakage. FGGS prepared with diluted thrombin solution can provide adequate adhesion strength for clinical use.

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Gelation dynamics and gel structure of fibrinogen

Cited by 30 publications

References 14 publications

Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels

Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

Effect of Thrombin Concentration on the Adhesion Strength and Clinical Application of Fibrin Glue-Soaked Sponge

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