Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro

Reis, Fabiana Navas; Francese, Monique Malta; Silva, Natara Dias Gomes da; Pelá, Vinícius Taioqui; Câmara, João Víctor Frazão; Trevizol, Juliana Sanches; Pieretti, Joana C.; Seabra, Amedea B.; Pessan, Juliano Pelim; Honório, Heitor Marques; Crusca, Edson; Marchetto, Reinaldo; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.jmbbm.2022.105549

Cited by 16 publications

(9 citation statements)

References 47 publications

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“…25 As mentioned above, several intracellular proteins delivered to saliva after exfoliation of oral mucosa cells have the potential to bind to hydroxyapatite, or participate in supramolecular aggregates that bind to the precursor proteins in the AEP. This has also been observed in other recent studies, [26][27][28][29][30][31] and could have a central role in protecting the tooth surface against acid dissolution. Hence, it is worthwhile noting that not only do secreted salivary proteins participate in AEP formation, but oral mucosa proteins, gingival crevicular fluid and even bacteria also act strongly on the enamel pellicle.…”

Section: This Process Is Based On the Epigenetic Mechanisms Associate...supporting

confidence: 83%

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

MARTINI,

RIOS,

DIONIZIO

et al. 2023

Braz. oral res.

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The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calciumbinding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution.

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Section: This Process Is Based On the Epigenetic Mechanisms Associate...supporting

confidence: 83%

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

MARTINI,

RIOS,

DIONIZIO

et al. 2023

Braz. oral res.

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“…Acquired pellicle engineering with acid-resistant proteins has emerged as an innovative and promising tool to prevent dental caries [18] and ETW [13,19,20]. Various studies, utilizing in vitro [21][22][23][24], in situ [19], and in vivo [13,20,25] protocols, have demonstrated the potential of CaneCPI-5, StN15, and HB in reducing dental demineralization. However, before dental products containing these proteins/peptide can be widely employed to manage dental caries and/or ETW, a deeper understanding of their mechanism of action and additional evidence on their efficacy, particularly from studies that closely mimic clinical conditions, are needed.…”

Section: Discussionmentioning

confidence: 99%

“…Ten volunteers (4 males and 6 females, age range [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] participated in this in vivo study. The volunteers met the following inclusion criteria: good health, both oral and general (could not present gingivitis, dental caries, periodontal disease, or any oral cavity changes that might alter oral fluids' composition), absence of restorations on the buccal surfaces of all upper and lower teeth, non-smokers, and not on medication that might decrease saliva flow.…”

Section: Ethical Aspects and Subjectsmentioning

confidence: 99%

Hemoglobin Protects Enamel against Intrinsic Enamel Erosive Demineralization

Carvalho,

Araújo,

Ventura

et al. 2024

Caries Res

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Introduction: This study investigated the changes in the acquired enamel pellicle (AEP) proteome when this integument is formed in vivo after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), and a statherin-derived peptide (StN15), or their combination and then exposed to an intrinsic acid challenge. The effectiveness of these treatments in preventing intrinsic erosion was also evaluated. Methods: Ten volunteers, after prophylaxis, in 5 crossover phases, rinsed with the following solutions (10mL, 1min): control (deionized water-H2O)-group 1, 0.1mg/mL CaneCPI-5- group 2, 1.0mg/mL HB- group 3, 1.88x10-5M StN15- group 4 or a blend of these- group 5. Following this, AEP formation occurred (2h) and an enamel biopsy (10µL, 0.01M HCl, pH 2.0, 10s) was conducted on one incisor. The biopsy acid was then analyzed for calcium (Arsenazo method). The vestibular surfaces of the other teeth were treated with the same acid. Acid-resistant proteins in the residual AEP were then collected and analyzed quantitatively via proteomics. Results: Compared to control, treatment with the proteins/peptide, mixed or isolated, markedly enhanced acid-resistant proteins in the AEP. Notable increases occurred in pyruvate kinase PKM (11-fold, CaneCPI-5), immunoglobulins and submaxillary gland androgen-regulated protein 3B (4-fold, StN15), Hb and lysozyme-C (2-fold, StN15). Additionally, a range of proteins not commonly identified in the AEP, but known to bind calcium or other proteins were in groups treated with the tested proteins/peptide either in isolation or as a mixture. The mean (SD, mM) calcium concentrations released from enamel were 3.67±1.48a, 3.11±0.72a, 1.94±0.57b, 2.37±0.90a and 2.38±0.45a for groups 1-5, respectively (RM-ANOVA/Tukey, p<0.05). Conclusions: Our findings demonstrate that all treatments, whether using a combination of proteins/peptides or in isolation, enhanced acid-resistant proteins in the AEP. However, only HB showed effectiveness in protecting against intrinsic erosive demineralization. These results pave the way for innovative preventive methods against intrinsic erosion, using "acquired pellicle engineering" techniques.

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“…The immobilization of proteins in the acquired pellicle has been proposed as a new measure for the prevention of ETW [Santiago et al, 2017;Carvalho et al, 2020;Martini et al, 2020;Taira et al, 2020;Frazão Câmara et al, 2021;Pelá et al, 2021a;Pelá et al, 2021b;Santos et al, 2021;Reis et al, 2023]. Among the poten-Fig.…”

Section: Discussionmentioning

confidence: 99%

Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro

et al. 2023

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The effect of solutions containing a statherin-derived peptide (Stn15pSpS) on the protection against enamel erosion in vitro was evaluated. Bovine enamel specimens were divided into 4 groups (n = 15/group): 1) Deionized water (negative control), 2) Elmex Erosion Protection™ (positive control), 3) 1.88 × 10-5 M Stn15pSpS and 4) 3.76 × 10-5 M Stn15pSpS. The solutions were applied on the specimens for 1 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH-cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The solutions were applied again during pH cycling, 2 times/day for 1 min after the first and last erosive challenges. Enamel loss (µm) was assessed by contact profilometry. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). The best protection against erosion was conferred by Elmex Erosion Protection that significantly differed from all the other treatments, followed by the solutions containing Stn15pSpS, regardless of the concentration. However, 3.76 × 10-5 M Stn15pSpS did not differ from the negative control. The solution containing the lower concentration of Stn15pSpS protected against erosion in vitro, which should be confirmed using protocols that more closely resemble the clinical condition.

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Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro

Cited by 16 publications

References 47 publications

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Hemoglobin Protects Enamel against Intrinsic Enamel Erosive Demineralization

Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro

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