Abstract. Prevalence of musculoskeletal pain is a major concern among manufacturing-based companies as they are still very reliant on manual labour. A better understanding whether musculoskeletal pain differ between gender and would enable organizational intervention to improve employees' health and safe working conditions. Therefore, the objective of this study is to determine the current prevalence of musculoskeletal pain among production workers and differences of musculoskeletal pain between gender and across age groups. A cross-sectional survey involving 324 shop-floor manufacturing workers was done using Cornell Musculoskeletal Discomfort Questionnaires (CMDQ). No significance differences were observed between female and male employees in terms of right hand, left hand, lower extremity, neck and lower back. However, there is a significance difference of pain at the upper extremity with female operators experienced more pain compared to their male counterparts. No significance differences were found among age categories for lower back, right hand, left hand, upper and lower extremity. However, neck pain and left hand was significantly different. The age effects indicate non linear association.