Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Avrushchenko, M. Sh.; Острова, И. В.; Grechko, Andrey V.

doi:10.15360/1813-9779-2017-5-44-57

Cited by 5 publications

(9 citation statements)

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“…According to our data obtained earlier, in male rats subjected to cardiac arrest of the same duration, similar post-resuscitation shifts take place in the population of Purkinje cells [18]. However, they occur earlier than in female rats specifically, by day 4 after resuscitation.…”

Section: Resultssupporting

confidence: 79%

“…Согласно полученным нами ранее данным, у самцов, перенесших остановку сердца такой же длительности, в популяции клеток Пуркинье происходят аналогичные постреанимационные сдвиги [18]. Однако они возникают раньше, чем у самокуже к 4-м суткам после реанимации.…”

Section: Resultsunclassified

“…1). На основании анализа гистограмм распределения нейронов по их оптической плотности и визуальной оценки выделяли соответствующие ранги для BDNF-негативных (BDNF -), слабо -и сильнопозитивных (BDNF + и BDNF ++ ) нейронов, в соответствии с тем, как было описано ранее [18].…”

Section: материал и методыunclassified

“…1). Based on the analysis of histograms of neurons distribution by their optic density and visual assessment, respective ranks were established for BDNF-negative (BDNF -), weakly -and strongly positive (BDNF + and BDNF ++ ) neurons, as described earlier [18].…”

Section: результаты и обсуждениеmentioning

confidence: 99%

“…Brain-derived neurotrophic factor (BDNF) impacts the neuroprotection and brain function recovery during various pathological conditions [10][11][12][13][14][15][16][17]. Earlier we studied postresuscitation changes of BDNF expression in highly sensitive to hypoxia neuronal populations in males [18]. That study revealed relation between BDNF expression shifts and postresuscitation death of neurons.…”

Section: Introductionmentioning

confidence: 99%

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Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Avrushchenko¹,

Острова²,

Grechko³

2017

rmt

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1 7 , 1 3 ; 5 44 w w w . r e a n i m a t o l o g y . c o mЦель: выявить половые особенности постреанимационных сдвигов экспрессии BDNF и сопряженных с ними процессов гибели нейронов.Материалы и методы. На разных сроках постреанимационного периода (1-, 4-, 7-, 14-е сутки) исследо-вали состояние высокочувствительных к гипоксии нейрональных популяций (пирамидные нейроны гиппо-кампа и клетки Пуркинье мозжечка) у белых половозрелых самок крыс, перенесших 10-минутную останов-ку системного кровообращения (пережатие сосудистого пучка сердца). Контролем служили ложноопериро-ванные животные. Проводили иммуногистохимическое выявление BDNF-иммунореактивных нейронов с последующим определением оптической плотности, числа клеток с разным уровнем экспрессии BDNF и общего числа нейронов на 1 мм длины их слоя. В работе использованы системы анализа изображений (ком-пьютер Intel, микроскоп Olympus BX-41, программы ImadgeScopeM, ImageJ 1,48v, Excel 2007). Статистиче-скую обработку данных проводили в программе Statistica 7.0. с использованием критериев λ Колмогорова-Смирнова, U-критерия Манна-Уитни и t-критерия Стьюдента.Результаты. Установлена динамика постреанимационных сдвигов BDNF-иммунореактивности высо-кочувствительных к гипоксии нейрональных популяций. Показано, что в популяции клеток Пуркинье у самок происходят изменения уровня экспрессии BDNF, что сопровождается гибелью нейронов. Выявлено, что эти сдвиги развиваются позднее, чем у самцов -к 7-м суткам постреанимационного периода. Суще-ственно, что гибели подвергаются только BDNF-негативные и слабопозитивные нервные клетки. В популя-ции пирамидных клеток гиппокампа у самок, в отличие от самцов, не происходило изменений BDNF-имму-нореактивности, и процесс гибели нейронов не развивался.Заключение. Выявлены гендерные особенности развития постреанимационных сдвигов уровня экспрес-сии BDNF и сопряженных с ними процессов гибели нейронов. Показано, что после остановки сердца одина-ковой длительности у самок постреанимационные сдвиги уровня экспрессии BDNF и процессы гибели ней-ронов выражены меньше, чем у самцов. В то же время, у животных обоего пола проявляются общие законо-мерности постреанимационных изменений мозга, свидетельствующие о взаимосвязи уровня экспрессии BDNF в нейронах с их устойчивостью к ишемии-реперфузии. Обсуждаются гендерные особенности повреж-дения мозга и их значение для понимания механизмов развития постгипоксических энцефалопатий. Ключевые слова: мозговой нейротрофический фактор (BDNF); гендерные особенности; постреанимацион-ный период; гибель нейронов; пирамидные клетки; гиппокамп; клетки Пуркинье; мозжечок; иммуногистохи-мия; оптическая плотность; морфометрический анализPurpose: to identify gender peculiarities of postresuscitation shifts in BDNF expression and neuronal death. Materials and Methods. At different points of the postresuscitation period (days 1-, 4-, 7-, and 14), the condition of highly sensitive to hypoxia neuronal populations (pyramidal neurons of hippocampus and Purkinje cells of cerebellum) were studied in white mature female rats exposed ...

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Section: Resultssupporting

confidence: 79%

Section: Resultsunclassified

Section: материал и методыunclassified

Section: результаты и обсуждениеmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Avrushchenko¹,

Острова²,

Grechko³

2017

rmt

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Morpho-Functional Changes of Thymus and Contents of Blood Lymphocyte Subpopulations in Female Wistar Rats With Different Resistance to Hypoxia in Systemic Inflammatory Response

et al. 2019

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Hypoxia and immune reactions are closely interrelated at molecular, cellular and organism levels, and the individuals differ in resistance to oxygen deficiency. Animals with high and low resistance to hypoxia have different adaptive capabilities and predisposition to the development of inflammatory diseases. Data on the individual characteristics of hypoxia resistance in female laboratory animals and humans, and its relationship to immune system reactions in both normal conditions and inflammatory diseases are not available in the literature. It is known, however, that acute infectious and inflammatory diseases develop at lesser rates and are less severe in women and female laboratory animals than in males, which can be explained by higher resistance of females to hypoxia. The aim of our study is to reveal the features of morpho-functional thymus changes, and subpopulation of peripheral blood lymphocytes in systemic inflammatory response induced by LPS administration to female Wistar rats with different resistance to hypoxia. Resistance of mature female Wistar rats to hypoxia was determined as a survival period in a ventilated lowpressure chamber simulating high altitude condition (11 500 m). The rats with a lifetime “at high altitude” of > 180 s have been classified as highly resistant to hypoxia, and the animals surviving for < 20 seconds were designated low-resistant. One month after determining the hypoxia resistance, the females were injected intraperitoneally with E. coli O26:B6 lipopolysaccharide (LPS) at a dose of 1.5 mg/kg during the dioestrus phase. The animals were withdrawn from the experiment by i/m Zoletyl injection (15 mg/kg) one day after LPS administration. The relative volume fractions of thymic cortex and medulla were evaluated; the areas of necrosis were determined in the liver, and the number of neutrophils in the interalveolar septa was counted in the lungs. The serum contents of corticosterone, testosterone, TGF-β were determined. A flow cytometry evaluation of the relative and absolute numbers was performed for major subpopulations of lymphocytes in peripheral blood. The number of apoptotically dying cells of the thymus was assessed. For statistical processing of the obtained data, the Statistica 8.0 software was applied, using criteria of multiple comparisons by Kruskal–Wallis and Dann. The differences were considered statistically significant at p < 0.05.In both high- and low-resistant to hypoxia females, the development of a systemic inflammatory -response was accompanied by a moderately severe thymic involution, apoptosis of thymocytes, an increase in the absolute number of NK, and rise of testosterone and corticosterone contents. LPS injection into low-resistant rats, if compared to females highly resistant to hypoxia, led to more severe manifestations of systemic inflammation, i.e., a pronounced inflammatory reaction in the lungs and a more extensive liver necrotic area accompanied by increased absolute numbers of regulatory T lymphocytes and T helper cells, and more pronounced thymic accidental involution with apoptotic death of thymocytes. Systemic manifestations of inflammation were less pronounced in hypoxia-resistant female rats, which was apparently associated with activation of lymphocyte migration from the thymus and blood to the inflammation focus, and development of more effective immune response.Conclusion: immune reactions in the systemic inflammatory response induced by LPS in female Wistar rats depend on individual resistance to hypoxia. These data should be used to develop approaches to personalized therapy of infectious and inflammatory diseases in women.

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Sex Differences in Systemic Inflammatory Response in Low Hypoxia-Resistant Rats

Tsvetkov,

Kosyreva,

Dzhalilova

et al. 2024

Morphology

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OBSERVATION. Individual differences in hypoxia tolerance and expression of the hypoxia-inducible factor, HIF-1α, were found in humans and laboratory animals. This allowed us to distinguish groups of animals with low and high resistance to oxygen deficiency. It was shown that low hypoxia-resistant animals are characterized by a more severe course of the systemic inflammatory response (SIR). Sex differences in the severity of SIRS in animals with low hypoxia tolerance have not been studied. OBJECTIVE To characterize morphological and molecular-biological features of lipopolysaccharide (LPS)-induced SIR in low hypoxia-resistant male and female Wistar rats. MATERIALS AND METHODS. Low hypoxia-resistant animals were distinguished by the time of “life” in a hypobaric barocamera at an altitude of 11500 m (less than 60 s). SWO was modeled by intraperitoneal injection of LPS at a dose of 1.5 mg/kg. One day later, morphologic changes in the lungs, liver, and thymus, as well as endotoxin levels, corticosterone, HIF1-α, and TGFβ concentrations in serum from low hypoxia-resistant female and male Wistar rats were evaluated. RESULTS. Differences were found in control groups in females and males of low hypoxia-resistant rats, characterized by higher HIF1-α, corticosterone and endotoxin levels in serum in females. Thus, in females 24 h after LPS injection the number of neutrophils in interalveolar septa of lungs was higher and acute accidental involution of thymus with narrowing of subcapsular zone without changes in volumetric fraction of cortical substance developed. Whereas in males widespread pathological changes in the liver and more pronounced manifestations of accidental involution accompanied by narrowing of the volume fraction of the thymus cortical substance were revealed. In the blood serum of females with SWO, the content of corticosterone increased, while in males the concentration of HIF1-α and the level of endotoxin increased, and the content of TGFβ decreased. CONCLUSIONS. Although female and male rats were characterized by low resistance to hypoxia, the course of LPS-induced SWO and the development of immune reactions in animals have sex differences.

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Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Cited by 5 publications

References 1 publication

Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Gender Peculiarities of Postresuscitation in the Expression of Brain-Derived Neurotrophic Factor (BDNF)

Morpho-Functional Changes of Thymus and Contents of Blood Lymphocyte Subpopulations in Female Wistar Rats With Different Resistance to Hypoxia in Systemic Inflammatory Response

Sex Differences in Systemic Inflammatory Response in Low Hypoxia-Resistant Rats

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