Gene expression and deletion analysis of mechanisms for electron transfer from electrodes to Geobacter sulfurreducens

Proteins involved in electron transfer to Fe(III) and Mn(IV) oxides by Geobacter sulfurreducens and Geobacter uraniireducens

“…1). PccH is also required for G. sulfurreducens to accept electrons from a poised cathode in a microbial fuel cell (Strycharz et al, 2011). Further investigation into the role of PccH in insoluble Fe(III) oxide reduction is necessary.…”

A Previously Uncharacterized, Nonphotosynthetic Member of the Chromatiaceae Is the Primary CO 2 -Fixing Constituent in a Self-Regenerating Biocathode

“…Cathodes hold great potential as a stable electron source to drive microbial metabolism (7); however, little is known about the underlying extracellular electron transfer (EET) pathways that could be exploited for biocathode functional engineering. Although biocathode EET has been demonstrated for a variety of microorganisms, including acetogens (5) and a methanogenic archaeon (6), studies aimed at identifying EET conduits from the electrode to cells have mostly been confined to the model organisms Geobacter (8) and Shewanella (9), due to the massive effort put forth to understand how these iron-reducing bacteria are able to catalyze EET at bioanodes (10)(11)(12). The ability of iron-reducing bacteria to reduce anodes led to the hypothesis that iron-oxidizing bacteria (FeOB) would be able to perform EET with cathodes, which has been demonstrated for at least two FeOB, Mariprofundus ferrooxydans and Rhodopseudomonas palustris (13,14).…”

The microbiology of microbial electrolysis cells

“…Interestingly the Mtr system is reversible and capable of accepting electrons from the electrode surface where the systems in Geobacter are not. OmcS and OmcZ do not affect electrode oxidation whereas GSU3274, a heme containing cytochrome does 27 . Furthermore, direct electron transfer differs when the extracellular electron acceptor is soluble.…”