Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions

Oliveira, Alexandre Tavares Duarte de; Lopes, Rita de Cássia Sobreira; Rodrigues, José Luiz

doi:10.1016/j.theriogenology.2005.03.019

Cited by 35 publications

(27 citation statements)

References 57 publications

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“…Hsp 70 family (70.1, 70.2, 70.8) and other small Hsps (10, 60) form the most important group of stress responsive elements in embryonic cells [38,39]. The relative importance of these proteins in oocyte/embryo growth and development could be potentially used as biomarkers for in vitro as well as in vivo studies concerning stressful conditions [40]. The rapid induction of Hsp70 and other small Hsps represents a unique feature of the physiological function of these molecules.…”

Section: Discussionmentioning

confidence: 99%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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Objective To determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5°C) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. Method The control group oocytes were cultured at 38.5°C for 24 h. The treatment 1 (T1) and 3 (T3) group oocytes were cultured at 40.5 and 41.5°C respectively, for the first 12 h and at 38.5°C for rest of the 12 h. However, treatment 2 (T2) and 4 (T4) group oocytes were cultured at 40.5 and 41.5°C for complete 24 h. Results Development of oocytes to blastocyst was severely compromised (p<0.001) when matured at 40.5 and 41.5°C for both exposure periods (12 h and 24 h). It was found that the cleavage rates, blastocyst yield and mean cell number decreased remarkably (p<0.001) in the treatment groups compared to control. The relative mRNA expression of heat shock protein (Hsp 70.1, 70.2, 70.8, 60, 10 and HSF1), pro-apoptotic (caspases-3, −7, −8, Bid and Bax) and oxidative stress (iNOS) related genes was significantly higher (p<0.05) in all the treatment groups compared to control. However, mRNA abundance of anti-apoptotic (Bcl-2, Mcl-1, Bcl-xl), glucose transport (Glut1, Glut3 and IGF1R), developmental competence (ZAR1 and BMP15) and oxidative stress (MnSOD) related genes was significantly decreased (p<0.05) in the treatment groups compared to control. Conclusion The present study clearly establishes that physiologically relevant elevated temperatures during in vitro meiotic maturation reduce developmental competence of bubaline oocytes.

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Section: Discussionmentioning

confidence: 99%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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“…In fact, studies of the embryonic secretome indicate that more than one factor is involved in promoting early embryonic development during culture in vitro [26]. Accordingly, it has been reported that embryo quality was greatly improved by culturing the embryos in reduced volumes and/or by increasing embryo density [27][28][29], implying that a detrimental developmental effect on cultured embryos was observed when the size of the droplet was increased. However, the results of our present study suggest that the development of mouse embryos is not improved by increasing the embryo density of culture (Tables 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Dai¹,

Xu²,

Wang³

et al. 2012

J Assist Reprod Genet

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“…Although lots of studies have dealt with differences in gene expression pattern between in vivo derived bovine embryos associated with high quality and in vitro derived embryos associated with poor quality, only few have been reported about the effects of varying embryo densities on bovine preimplantative gene expression. To our knowledge, only de Oliveira et al (2005) reported the effect of different embryo densities on transcript expression of certain genes. The relative abundance of HSPA1A transcripts in day-7 blastocysts was found to be higher in embryos produced in embryo densities of 1:5 than in 1:20, whereas no difference in terms of SLC2A1 mRNA was observed.…”

Section: Introductionmentioning

confidence: 99%

Effect of the microenvironment and embryo density on developmental characteristics and gene expression profile of bovine preimplantative embryos cultured in vitro

Hoelker¹,

Rings²,

Lund³

et al. 2009

Reproduction

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The Well of the Well (WOW) system has been developed to culture embryos in small groups or to track the development of single embryos. In the present study, we aimed to examine the effects of the microenvironment provided by the WOW system and embryo density on developmental rates, embryo quality and preimplantative gene expression profile of the resulting embryos. Embryos cultured in a group of 16 reached the blastocyst stage at a significantly lower level than zygotes cultured in a group of 50 (22.2 vs 30.3%), whereas zygotes cultured in WOW were able to compensate against low embryo densities, reaching a blastocyst rate as high as embryos cultured in a group of 50 (31.3 vs 30.3%). Moreover, embryos derived from WOW culture did not differ in terms of differential cell counts and apoptotic cell index compared with controls. The gene expression analysis revealed 62 transcripts to be upregulated and 33 transcripts to be downregulated by WOW culture. Comparing the in vivo derived blastocysts with the blastocysts derived from WOW culture, and group culture, expression of ATP5A1, PLAC8 and KRT8 was more similar to the embryos derived from WOW culture, whereas expression of S100A10 and ZP3 genes was more similar to blastocysts cultured in a group. In conclusion, microenvironment as well as embryo density significantly affected developmental rates. While subsequent blastocysts did not differ in terms of differential cell counts and apoptotic cell index, significant differences were observed in terms of the relative abundance of transcripts in the resulting embryos.

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Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions

Cited by 35 publications

References 57 publications

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Effect of the microenvironment and embryo density on developmental characteristics and gene expression profile of bovine preimplantative embryos cultured in vitro

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