Gene expression by marrow stromal cells in a porous collagen–glycosaminoglycan scaffold is affected by pore size and mechanical stimulation

Abstract

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A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering

“…Studies in this laboratory have also shown that CG scaffolds may support in vitro osteogenesis (differentiation process of a progenitor cell into a mature bone forming osteoblast) of rat mesenchymal stem cells and mouse osteogenic cell line MC3T3 Byrne et al, 2008). To date however, no studies have examined the ability of this scaffold to support long term human osteogenesis.…”

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

“…Due to the highly porous nature of the scaffolds used in this study, we believe that the cell density was sufficient to investigate the interaction of cells and scaffolds. This cell seeding density was based on previous work carried out in our laboratory and has been used both in vitro and in vivo (Farrell et al, 2006, Byrne et al, 2008, Lyons et al, 2010b, Alhag et al, 2011. A higher cell density would create a phenomenon whereby cell-cell interaction out-weighed cell-matrix interaction resulting in an imprecise interpretation of the response of MSCs to the differing compositions.…”

Tissue differentiation in an in vivo bioreactor: in silico investigations of scaffold stiffness

“…The cells were allowed to migrate, proliferate, apoptose, differentiate and synthesise new extracellular matrices while new capillaries invaded the chamber, depending on the surrounding mechanical environment. The scaffold material was included in the chamber by superimposing cross sections (pixels) of processed µCT scans of a highly porous (>90%) collagen GAG-scaffold, which has been used in bone tissue engineering [17][18][19][20], on the lattice points (Fig. 1e).…”