Tissue differentiation in an in vivo bioreactor: in silico investigations of scaffold stiffness

Khayyeri, Hanifeh; Checa, Sara; Tägil, Magnus; O’Brien, Fergal J.; Prendergast, Patrick J.

doi:10.1007/s10856-009-3973-0

Cited by 24 publications

(15 citation statements)

References 31 publications

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“…1), were developed to investigate the mechanical conditions within the callus. In addition, a latticebased mechano-biological model that explicitly includes migration, proliferation and differentiation of the cells (Byrne et al, 2007;Prendergast, 2009a, 2009b;Prendergast et al, 2010;Sandino et al, 2010;Khayyeri et al, 2009Khayyeri et al, , 2010 was implemented to simulate the bone healing process. Model predictions were compared with histological sections at several time points post-surgery.…”

Section: Discussionmentioning

confidence: 99%

Inter-species investigation of the mechano-regulation of bone healing: Comparison of secondary bone healing in sheep and rat

Checa

Prendergast

Duda

2011

Journal of Biomechanics

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Section: Discussionmentioning

confidence: 99%

Inter-species investigation of the mechano-regulation of bone healing: Comparison of secondary bone healing in sheep and rat

Checa

Prendergast

Duda

2011

Journal of Biomechanics

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“…Modelling of bioreactors allows also, to determine the optimal parameters governing the scaffold performances. Khayyeri et al 144 combined a lattice-based model of a 3D porous scaffold construct derived from micro CT and a mechanobiological simulation of a bone chamber experiment to investigate the effect of scaffold stiffness on tissue differentiation inside the chamber. The results indicate that higher scaffold stiffness, holding pore structure constant, enhances bone formation.…”

Section: Mechanobiologymentioning

confidence: 99%

Finite Element Method (FEM), Mechanobiology and Biomimetic Scaffolds in Bone Tissue Engineering

Boccaccio¹,

Ballini²,

Pappalettere³

et al. 2011

Int. J. Biol. Sci.

138

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Techniques of bone reconstructive surgery are largely based on conventional, non-cell-based therapies that rely on the use of durable materials from outside the patient's body. In contrast to conventional materials, bone tissue engineering is an interdisciplinary field that applies the principles of engineering and life sciences towards the development of biological substitutes that restore, maintain, or improve bone tissue function. Bone tissue engineering has led to great expectations for clinical surgery or various diseases that cannot be solved with traditional devices. For example, critical-sized defects in bone, whether induced by primary tumor resection, trauma, or selective surgery have in many cases presented insurmountable challenges to the current gold standard treatment for bone repair. The primary purpose of bone tissue engineering is to apply engineering principles to incite and promote the natural healing process of bone which does not occur in critical-sized defects. The total market for bone tissue regeneration and repair was valued at $1.1 billion in 2007 and is projected to increase to nearly $1.6 billion by 2014.Usually, temporary biomimetic scaffolds are utilized for accommodating cell growth and bone tissue genesis. The scaffold has to promote biological processes such as the production of extra-cellular matrix and vascularisation, furthermore the scaffold has to withstand the mechanical loads acting on it and to transfer them to the natural tissues located in the vicinity. The design of a scaffold for the guided regeneration of a bony tissue requires a multidisciplinary approach. Finite element method and mechanobiology can be used in an integrated approach to find the optimal parameters governing bone scaffold performance.In this paper, a review of the studies that through a combined use of finite element method and mechano-regulation algorithms described the possible patterns of tissue differentiation in biomimetic scaffolds for bone tissue engineering is given. Firstly, the generalities of the finite element method of structural analysis are outlined; second, the issues related to the generation of a finite element model of a given anatomical site or of a bone scaffold are discussed; thirdly, the principles on which mechanobiology is based, the principal theories as well as the main applications of mechano-regulation models in bone tissue engineering are described; finally, the limitations of the mechanobiological models and the future perspectives are indicated.

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“…The simulation results suggested that the seeding process and mechanical stimulation were key parameters when engineering large bone tissue volumes. Table summarizes some of other recent studies on computational scaffold design …”

Section: Computational Scaffold Designmentioning

confidence: 99%

Current strategies in multiphasic scaffold design for osteochondral tissue engineering: A review

Yousefi

Hoque

Prasad³

et al. 2014

J. Biomed. Mater. Res.

176

157

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The repair of osteochondral defects requires a tissue engineering approach that aims at mimicking the physiological properties and structure of two different tissues (cartilage and bone) using specifically designed scaffold-cell constructs. Biphasic and triphasic approaches utilize two or three different architectures, materials, or composites to produce a multilayered construct. This article gives an overview of some of the current strategies in multiphasic/gradient-based scaffold architectures and compositions for tissue engineering of osteochondral defects. In addition, the application of finite element analysis (FEA) in scaffold design and simulation of in vitro and in vivo cell growth outcomes has been briefly covered. FEA-based approaches can potentially be coupled with computer-assisted fabrication systems for controlled deposition and additive manufacturing of the simulated patterns. Finally, a summary of the existing challenges associated with the repair of osteochondral defects as well as some recommendations for future directions have been brought up in the concluding section of this article.

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Tissue differentiation in an in vivo bioreactor: in silico investigations of scaffold stiffness

Cited by 24 publications

References 31 publications

Inter-species investigation of the mechano-regulation of bone healing: Comparison of secondary bone healing in sheep and rat

Inter-species investigation of the mechano-regulation of bone healing: Comparison of secondary bone healing in sheep and rat

Finite Element Method (FEM), Mechanobiology and Biomimetic Scaffolds in Bone Tissue Engineering

Current strategies in multiphasic scaffold design for osteochondral tissue engineering: A review

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